Biochemical composition of carious dentin and different layers of sound dentin

Magnus, Larissa; Maltz, Marisa; Bavaresco, Caren Serra; Bastos, Lucelen Fontoura; Hashizume, Lina Naomi

doi:10.2334/josnusd.55.133

Cited by 5 publications

(6 citation statements)

References 26 publications

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“…The statistical analysis revealed interaction of both factors, as the superficial dentin in the sclerotic group presented lower concentration of Ca in weight %. Previous studies have reported the mineral composition of sclerotic dentin [27][28][29][30], with lower concentration of calcium compared to sound dentin. Those results are consistent with dental caries pathology, more specific to the demineralization process, which is characterized by mineral loss [28,31,32].…”

Section: Discussionmentioning

confidence: 94%

Determination of chemical elements and cross-section hardness of sclerotic darkened human dentin

Souza

Meirelles

Duque

et al. 2017

BDS

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Objective: The aim of this study was to assess the amount of chemical elements (Ca, O, C, P, Fe, and Mg) and the cross-section hardness of sclerotic darkened dentin in human teeth. Material and Methods: The study was approved by the local IRB and ten extracted teeth (five sound and five presenting sclerotic darkened dentin) were used. Tooth was sectioned mesiodistally and each half was used for each test. Amount of chemical elements (%w) was determined by energy dispersive X-ray spectroscopy (EDS) in three different dentin areas (shallow, medium, or deep sound or sclerotic dentin). Knoop microhardness was determined at the same EDS areas. Data were analyzed by two-way ANOVA and multiple comparison tests, with significance level at 5%. Results: No difference on microhardness was detected between sound and sclerotic dentin (p = 0.743) and also among dentin depths (p = 0.837). Lower Ca (p = 0.024) and higher C (p = 0.015) amounts were found at superficial sclerotic dentin. Increased Mg content (p < 0.001) was detected in sound dentin. Conclusion: It was concluded darkened sclerotic dentin presents similar cross-section microhardness to sound dentin. The assessed chemical elements were similarly present in sound or sclerotic dentin, except for Mg, which was present higher concentration in sound dentin. Ca and P were lower in superficial sclerotic dentin. ReSumoObjetivo: O objetivo deste estudo foi avaliar a quantidade de elementos químicos (Ca, O, C, P, Fe e Mg) e a microdureza superficial da dentina esclerótica escurecida em dentes humanos. Material e Métodos: O estudo foi aprovado pelo Comitê de Ética local e dez dentes extraídos (cinco hígidos e cinco apresentando dentina esclerosada) foram utilizados. Os dentes foram seccionados no sentido mésiodistal e cada metade foi utilizada para cada teste. A quantidade de elementos químicos (%w) foi determinada pela espectroscopia de raio-x por dispersão em energia (EDS) em três áreas dentinárias diferentes (superficial, média ou profunda). Estas mesmas áreas tiveram a microdureza superficial Knoop determinada. Os dados foram analisados por ANOVA dois fatores e teste de comparações múltiplas, com nível de significância de 5%. Resultados: Nenhuma diferença quanto à microdureza foi detectada entre a dentina hígida e esclerótica (p = 0,743) e nem em relação às profundidades (p = 0,837). Menor quantidade de Ca (p = 0,024) e maior quantidade de C (p = 0,015) foram encontradas na dentina esclerótica superficial. O aumento do conteúdo de Mg (p < 0,001) foi detectado na dentina hígida. Conclusão: Pode-se concluir que a dentina esclerótica apresenta microdureza superficial similar à dentina hígida. Os elementos químicos estudados apresentaram-se igualmente para os dois tipos de dentina, exceto para o Mg, o qual estava em maior quantidade na dentina hígida. O cálcio e o fósforo apresentaram quantidades reduzidas na dentina esclerótica superficial.

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Section: Discussionmentioning

confidence: 94%

Determination of chemical elements and cross-section hardness of sclerotic darkened human dentin

Souza

Meirelles

Duque

et al. 2017

BDS

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“…An attempt to overcome this limitation, however, was by the use of the adjusted grey density values which attempted to unify the grey density scale across the cases by unifying the density of subcrestal dentine. Although it is not expected that all patients truly have similar density of dentine, the density of subcrestal dentine is expected to be the tissue with the least amount of variation amongst patients because of the stability of dental structures which are not exposed to the environment (Magnus et al 2013). As such, the use of adjusted grey density values may actually increase the generalizability of the study results because the variable exposure parameters used in clinical practice are accounted for within the study.…”

Section: Discussionmentioning

confidence: 99%

Accuracy of mean grey density values obtained with small field of view cone beam computed tomography in differentiation between periapical cystic and solid lesions

et al. 2020

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Aim To determine if small and medium field of view (FOV) cone beam computed tomography (CBCT) adjusted grey density values can be used to distinguish between periapical cystic and solid lesions. Methodology Fifty‐seven patients with periapical lesions having retrievable small or medium FOV CBCT images and biopsy samples were included. Two oral and maxillofacial pathologists examined the biopsy samples to provide the gold standard diagnosis of cystic or solid lesion. From the CBCT images, two independent examiners recorded the minimum adjusted grey density value of each lesion twice. Intra‐examiner and inter‐examiner reliability of the measurements were analysed, and sensitivity, specificity and accuracy of the minimum grey values in distinguishing a solid from cystic lesion were calculated. A receiver operating curve for diagnostic ability of adjusted grey density values to differentiate between periapical cystic and solid lesions was obtained, and the area under the curve (AUC) was calculated. Results The intra‐ and inter‐examiner reliability of the grey density values of the lesions and dentine were excellent. The AUC was 0.44 (P‐value = 0.45). The adjusted grey density value with the greatest accuracy for differentiating between cystic and solid lesions had an accuracy, sensitivity and specificity of 0.54, 1.00 and 0.075, respectively. Conclusions Small FOV CBCT adjusted grey density values obtained by the device used in the study could not distinguish between periapical cystic and solid lesions. Further developments in CBCT devices are needed to improve the accuracy of grey density measurements.

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“…Divergences between the chemical composition of carious and healthy teeth could also result from environmental factors (diet and pollution), the impact of other diseases, different patient ages in subsequent studies, and the use of different measurement tools. The main dentine components – calcium, inorganic phosphorous, and fluorine – play a key role in tooth demineralisation and remineralisation [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

Changes in the chemical composition of mineralised teeth in children after antineoplastic treatment

Krasuska-Sławińska¹,

Dembowska‐Bagińska²,

Brożyna³

et al. 2018

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IntroductionChemotherapy, neoplasms, and their complications linked to malabsorption, malnutrition, and metabolic disorders may lead to improper tooth development and frequent severe caries in patients during/after antineoplastic treatment and to a more frequent improper tooth development in patients undergoing chemotherapy during odontogenesis. However, the causes of these abnormalities remain unknown; there are no studies on the impact of antineoplastic treatment and its complications on the chemical composition of mineralised teeth.Aim of the studyTo compare the chemical composition of mineralised teeth extracted due to complicated caries in children after chemotherapy, and of teeth extracted due to orthodontic treatment in generally healthy children.Material and methodsThe treatment group included five teeth extracted due to complicated caries in children after antineoplastic treatment. The control group included five teeth extracted due to orthodontic treatment in generally healthy children. The chemical composition of enamel, dentine, cementum, interior of the canal, and enamel abnormalities in teeth extracted from patients after chemotherapy and in generally healthy patients were assessed with energy-dispersive X-ray spectroscopy. Results were analysed statistically.ResultsThe magnesium (Mg) and zinc (Zn) mass contents in the enamel of patients after chemotherapy increased and so did the calcium (Ca) to phosphorus (P) ratio when compared to controls. Areas with abnormal enamel in patients after chemotherapy had lower concentrations of Ca and P, and higher concentrations of trace elements (Mg, Cl, and Na). The levels of the assessed elements in dentine, cementum, and inside the canal were similar in both groups of teeth.

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Biochemical composition of carious dentin and different layers of sound dentin

Cited by 5 publications

References 26 publications

Determination of chemical elements and cross-section hardness of sclerotic darkened human dentin

Determination of chemical elements and cross-section hardness of sclerotic darkened human dentin

Accuracy of mean grey density values obtained with small field of view cone beam computed tomography in differentiation between periapical cystic and solid lesions

Changes in the chemical composition of mineralised teeth in children after antineoplastic treatment

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