Biochemical characterization of three nucleopolyhedrovirus isolates of <i>Spodoptera exigua</i> and <i>Mamestra brassicae</i>

Murillo, Luis Gabriel; Muñóz,; Lipa,; Caballero,

doi:10.1046/j.1439-0418.2001.00533.x

Cited by 25 publications

(18 citation statements)

References 25 publications

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“…Therefore it may be one of the most infective strains. The idea that Asia is a potential source of new and interesting MNPV strains, proposed by Murillo et al (2), supports our results.…”

Section: Discussionsupporting

confidence: 93%

The First Record of Nucleopolyhedrovirus Isolated from the Gypsy Moth, Lymantria dispar (Lepidoptera, Lymantriidae) in Turkey

Yaman¹,

Bekircan²,

Radek³

et al. 2012

Turkiye Parazitol Derg

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Objective: The gypsy moth, Lymantria dispar L. (Lepidoptera, Lymantriidae) is a common pest of forests and fruit trees throughout the world. This insect is also a major serious pest in Turkey. Nowadays L. dispar can be managed by biological control methods especially, using entomopathogenic viruses. The aim of this study is to characterize entomopathogenic viruses and is the first record of nucleopolyhedrovirus isolated from the gypsy moth in Turkey. Methods: PIBs obtained from infected larvae were measured and photographed using an Olympus BX51 microscope with a DP-25 digital camera and a DP2-BSW Soft Imaging System and examined with a Philips 208 electron microscope (TEM). Results: The virus had the typical characteristics of nucleopolyhedroviruses. The dimension of the polyhedral inclusion bodies (PIBs) was 2.03±0.25 µm. PIBs varied in size from 1.65 to 2.21 µm and were usually polygonal in shape. Virions in PIBs contained 1 to 8 nucleocapsids per virion. The size of the viral particles was 366.67±54.72 (312-500) x 42.95±6.12 (30-47) nm. Conclusion: The isolation and characterization of a pure isolate of Lymantria dispar multinucleopolyhedrovirus (LdMNPV-TR) from Turkey is presented for the first time. (Turkiye Parazitol Derg 2012; 36: 92-5)

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“…Therefore it may be one of the most infective strains. The idea that Asia is a potential source of new and interesting MNPV strains, proposed by Murillo et al (2), supports our results.…”

Section: Discussionsupporting

confidence: 93%

The First Record of Nucleopolyhedrovirus Isolated from the Gypsy Moth, Lymantria dispar (Lepidoptera, Lymantriidae) in Turkey

Yaman¹,

Bekircan²,

Radek³

et al. 2012

Turkiye Parazitol Derg

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“…Identification by means of restriction endonuclease (REN) analysis has been used to detect variants Erlandson et al 2007) and to distinguish NPVs originating from different insects (Murillo et al 2001). More recently, the polymerase chain reaction (PCR) and DNA sequencing have facilitated phylogenetic and taxonomic analyses of NPVs (Lange et al 2004;Jehle et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Biological activity and identification of nucleopolyhedroviruses isolated from Mythimna separata and Spodoptera litura in Japan

et al. 2009

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We compared the infectivity of two nucleopolyhedroviruses (NPVs), MyseNPV G isolated from Mythimna separata (Walker) (Lepidoptera: Noctuidae) and SpltNPV S isolated from Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). MyseNPV G was more pathogenic against M. separata than against S. litura. Although SpltNPV S was more pathogenic than MyseNPV G against S. litura, it did not infect M. separata. Restriction endonuclease (REN) analysis of viral genomic DNA revealed that the two NPVs have quite different REN profiles. Based on nucleotide sequences of the coding regions of polyhedrin, lef-8 and lef-9, SpltNPV S was closely related to other SpltNPV isolates, whereas MyseNPV G appeared to belong to the Mamestra NPV group, and was distinct from a Chinese isolate of Leucania (=Mythimna) separata NPV. The potential of MyseNPV G and SpltNPV S to control pest insects is discussed.

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“…However, BmNPV infection has commonly occurred in silk-producing areas. Diverse baculovirus genomes can undergo deletion, point mutation, and recombination 20 . Likewise, the BmNPV genome undergoes similar processes.…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of a new Bombyx mori nucleopolyhedrovirus strain isolated from Yunnan, China

Fen-fen¹,

Zhang²,

Shao³

et al. 2017

ScienceAsia

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ABSTRACT:Bombyx mori nucleopolyhedrovirus (BmNPV) is a silkworm pathogen that causes serious economic losses in sericulture. In this study, a new BmNPV strain, designated as BmNPV-YN1, from Yunnan (China), was isolated and identified through molecular identification. Sequence analysis results revealed that polyhedrin (polh) presented the highest identity among various BmNPVs; this gene indicated that BmNPV-YN1 is a BmNPV strain. The baculovirus repeated-ORF (bro) genes of this isolate were different from those of six BmNPV strains (BmNPV-T3, BmNPV-ZJ, BmNPV-GX, BmNPV-India, BmNPV-Cubic, and BmNPV-Brazilian). The BmNPV-YN1 bro family includes bro-a, bro-b, broc, bro-d, and bro-e, which are similar to those of BmNPV-T3. Sequence alignment results further showed the differences in the composition and sequences of the bro family members among various strains. The bro-d sequences were also phylogenetically analysed. Results demonstrate that BmNPV-YN1, BmNPV-Cubic, and BmNPV-India are closely related. Despite this close relationship, BmNPV-YN1 belongs to a distinct cluster. The BmNPV DNA was subjected to restriction endonuclease analysis and the obtained results provided additional evidence supporting the identity of the novel BmNPV-YN1 strain. Indeed, BmNPV-YN1 isolated from Yunnan is a new BmNPV strain. The proposed method can be used to rapidly and accurately detect and identify BmNPVs strains.

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Biochemical characterization of three nucleopolyhedrovirus isolates of Spodoptera exigua and Mamestra brassicae

Cited by 25 publications

References 25 publications

The First Record of Nucleopolyhedrovirus Isolated from the Gypsy Moth, Lymantria dispar (Lepidoptera, Lymantriidae) in Turkey

The First Record of Nucleopolyhedrovirus Isolated from the Gypsy Moth, Lymantria dispar (Lepidoptera, Lymantriidae) in Turkey

Biological activity and identification of nucleopolyhedroviruses isolated from Mythimna separata and Spodoptera litura in Japan

Isolation and identification of a new Bombyx mori nucleopolyhedrovirus strain isolated from Yunnan, China

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