Biochemical characterization of microalgal biomass from freshwater species isolated in Alberta, Canada for animal feed applications

Tibbetts, Sean M.; Whitney, Crystal G.; Macpherson, Margaret; Bhatti, Shabana; Banskota, Arjun H.; Stefanova, Roumiana; McGinn, Patrick J.

doi:10.1016/j.algal.2014.11.011

Cited by 88 publications

(57 citation statements)

References 58 publications

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“…Mild heating of native proteins causes disruption of secondary and tertiary structures, which allows proteins to unfold, permitting for more efficient enzymatic digestion. Although the amino acid profiles of microalgal proteins are generally high relative to other common plant proteins [9,14,31], the functional properties of algal proteins are poorly understood. Chronakis [27] demonstrated that Spirulina proteins are highly viscous at acidic pH but become less viscous and more highly soluble at higher pH levels and this could affect the results from the various in vitro protein digestion assays used.…”

Section: Monogastric Animalsmentioning

confidence: 99%

“…(AB04-C-F-PLM02) and M. reisseri (AB05-C-U-BBM02). Isolation conditions, 18S gene sequence identification, screening criteria, mass cultivation, harvesting and processing and biochemical characterization are fully described in Tibbetts et al [9]. Proximate and caloric content of whole and lipidextracted biomass are presented in Table 1.…”

Section: Microalgal Biomassmentioning

confidence: 99%

“…Proximate composition and caloric content of WAB and LEB used for in vitro digestion studies is described in Tibbetts et al [9]. Methane content of headspace gas samples obtained from in vitro ruminal fermentation studies was determined according to Burton et al [25] using gas chromatography (model Star 3800, Varian, Mississauga, ON) equipped with TCD, ECD and FID detectors in series and a Combi-PAL autosampler.…”

Section: Analytical Techniquesmentioning

confidence: 99%

“…Due to the difficulties in extrapolating nutritional value results between monogastric and ruminant animals, separate in vitro digestion techniques are required, however, both can provide valuable data on bioavailability of dietary protein and energy from novel feed ingredients and may also provide a preliminary selection of treatments prior to undertaking costly in vivo feeding trials. The present study is the second in a series of projects designed to evaluate the nutritional value of four freshwater chlorophytic microalgae species isolated in Northern Alberta, Canada for animal feed applications [9]. The main objective was to generate novel in vitro digestion data of whole algal biomass (WAB) and lipid-extracted biomass (LEB) for both monogastric and ruminant livestock including protein solubility (PS), dilute pepsin digestibility (DPD), two-phase gastric/pancreatic protein digestibility (GPD Protein ) and energy digestibility (GPD Energy ), ruminal organic matter digestibility (OMD), apparent metabolizable energy (aME) content and methane production.…”

Section: Introductionmentioning

confidence: 99%

“…and Micractinium reisseri [7] and have been mass cultivated in enclosed photobioreactors [8]. The first in a series of studies with this algal biomass showed them to have similar growth characteristics, proximate compositions, favorable essential amino acid and fatty acid profiles, attractive minerals and trace element compositions and were devoid of contaminating heavy metals [9]. However, there were notable differences in their carbohydrate compositions with respect to starch and fiber, which could greatly affect their extent of digestion and ultimately their overall nutritional value when fed to target animal species.…”

Section: Introductionmentioning

confidence: 99%

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In vitro digestion of microalgal biomass from freshwater species isolated in Alberta, Canada for monogastric and ruminant animal feed applications

et al. 2016

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READ THESE TERMS AND CONDITIONS CAREFULLY BEFORE USING THIS WEBSITE.http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/copyright Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=bced7503-7e47-4b06-8011-600c65ad0b5e http://nparc.cisti-icist.nrc-cnrc.gc.ca/fra/voir/objet/?id=bced7503-7e47-4b06-8011-600c65ad0b5eIn vitro digestion of microalgal biomass from freshwater species isolated in Alberta, Canada for monogastric and ruminant animal feed applications In vitro digestion studies were conducted to examine the potential nutritional value of whole (WAB) and lipidextracted biomass (LEB) from freshwater microalgae from Alberta, Canada. For WAB, protein solubility (PS) was statistically highest and the same (P = 0.109) for Chlorella vulgaris at 84% and Micractinium reisseri at 78%, lowest (P b 0.001) for Nannochloris bacillaris at 64% and intermediate for Tetracystis sp. at 73%. Dilute pepsin digestibility (DPD) was highest (P b 0.001) for C. vulgaris at 80% and lowest (P b 0.001) for N. bacillaris and Tetracystis sp. at 60-64%, which were the same (P = 0.075) and M. reisseri was intermediate at 72%. Twophase gastric/pancreatic digestibility of protein (GPD Protein ) and energy (GPD Energy ) were highest (P b 0.001) for M. reisseri at 78 and 57%, respectively, lowest (P b 0.001) for N. bacillaris and Tetracystis sp. at 49-52 and 41-43%, respectively, which were the same (P = 0.101 and 0.058, respectively) and C. vulgaris was intermediate at 69 and 52%, respectively. For LEB, PS was highest (P b 0.001) and the same (P = 0.088) for C. vulgaris and M. reisseri at 72-76%; which were higher (P b 0.001) than N. bacillaris and Tetracystis sp. at 60-62%, which were the same (P = 0.405). Similarly, DPD was highest (P b 0.001) and the same (P = 1.000) for C. vulgaris and M. reisseri both at 69%; which were higher (P b 0.001) than N. bacillaris and Tetracystis sp. at 58-62%, which were the same (P = 0.083). GPD Protein was highest (P b 0.001) and the same (P = 0.944) for M. reisseri and C. vulgaris at 79-80%, lowest (P b 0.001) for N. bacillaris at 50% and intermediate for Tet...

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Section: Monogastric Animalsmentioning

confidence: 99%

Section: Microalgal Biomassmentioning

confidence: 99%

Section: Analytical Techniquesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro digestion of microalgal biomass from freshwater species isolated in Alberta, Canada for monogastric and ruminant animal feed applications

et al. 2016

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Phycoremediation and simultaneous production of protein‐rich algal biomass from aquaculture and agriculture wastewaters

Bhatti

Richards

Wall

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUND: Microalgae cultivation in wastewaters is a potential remediation technology for converting residual nutrients into valuable biomass for biofuels, fertilizers, animal and aquaculture feeds, and bio-based chemicals. The objective of this study was to determine the potential for microalgae phycoremediation of aquaculture and agriculture wastewaters for sustainable production of nutrient-rich algal biomass.RESULTS: Thirty-seven aquaculture and agriculture wastewaters were evaluated as growth media for two freshwater, chlorophytic microalgae strains (Chlorella sorokiniana SMC14M and Scenedesmus sp. AMDD). Although nutrient levels and physicochemical parameters of the wastewaters varied widely, phycoremediation efficiency of nitrogen and phosphorous was high (> 70%) for a large majority of them, with both microalgae strains. Photobioreactor cultivation (300 L) of C. sorokiniana grown on finfish aquaculture wastewater (CA2) and standard growth medium (SGM) exhibited similar biomass production levels and nutritional compositions of moisture (4.9-5.0%), ash (5.9-6.1%), nitrogen (8.6-8.8%), protein (41.0-42.1%), lipid (8.6-8.8%), carbohydrate (38.3-39.3%) and gross energy (22.2-22.3 MJÁkg −1 ) with highly similar fatty acid and amino acid profiles.CONCLUSIONS: This study demonstrates that the use of freshwater microalgae to remediate various aquaculture and agriculture wastewaters, while producing valuable nutrient-rich biomass, is feasible. We have found that a particular finfish aquaculture wastewater (CA2) contains all of the nutrients required for rapid microalgae growth, avoiding the need for expensive commercial fertilizers. The biochemical composition of C. sorokiniana grown on CA2 aquaculture wastewater has good potential for use as a nutrient-rich ingredient for animal and aquaculture feeds, fertilizers products, biofuels and other applications, resulting in remediated water that could be reused.

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Characterization of halotolerant microalga isolated from waterlogged habitats: Deciphering the biochemical profiling and unraveling the molecular identity

Sharma,

Kaur,

Gupta Phutela

et al. 2024

J Basic Microbiol

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The primary objective of this study was to comprehensively explore the biochemical profile of the novel halotolerant microalgae strain, biogas laboratory scenedesmus (BGLRS), previously isolated from waterlogged regions in the southwest zone of Punjab, India. To achieve this, three distinct drying methods viz. freeze‐drying, oven‐drying, and shade‐drying were employed and biochemical composition and antioxidant analyses on the microalgal biomass were conducted. Utilizing advanced analytical techniques, including high‐performance liquid chromatography (HPLC), inductively coupled plasma‐atomic emission spectroscopy (ICP‐AES), and gas chromatography‐mass spectroscopy (GC‐MS) on freeze‐dried biomass, its carbohydrate profile, micronutrient composition, and presence of bioactive compounds with potential therapeutic and nutraceutical significance were sought to unravel. Among the drying methods evaluated, freeze‐drying exhibited the most promising experimental results, prompting its selection for further investigation. Notably, ICP‐AES unveiled elevated concentrations of essential elements such as calcium, iron, magnesium, and phosphorus in BGLRS, with negligible traces of heavy metals, underscoring its safety for human consumption. GC‐MS analysis further divulged the existence of numerous biologically active compounds, indicating potential applications in medical and nutraceutical fields. Through molecular identification using sequencing of the internal transcribed spacer (ITS) region, a close taxonomic resemblance between BGLRS and Scenedesmus sp. MKB was established, solidifying its unique position within the microalgal taxonomy. The deposition of ITS sequences into the NCBI GenBank, obtaining accession number MN796425, attests to the rigor and transparency of this research. Overall, these findings strongly suggest that microalgae BGLRS possesses high‐quality biochemical attributes of significant therapeutic and nutraceutical importance.

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Biochemical characterization of microalgal biomass from freshwater species isolated in Alberta, Canada for animal feed applications

Cited by 88 publications

References 58 publications

In vitro digestion of microalgal biomass from freshwater species isolated in Alberta, Canada for monogastric and ruminant animal feed applications

In vitro digestion of microalgal biomass from freshwater species isolated in Alberta, Canada for monogastric and ruminant animal feed applications

Phycoremediation and simultaneous production of protein‐rich algal biomass from aquaculture and agriculture wastewaters

Characterization of halotolerant microalga isolated from waterlogged habitats: Deciphering the biochemical profiling and unraveling the molecular identity

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