Biochemical characterization of an alkaline surfactant-stable keratinase from a new keratinase producer, Bacillus zhangzhouensis

Moridshahi, Roghyeh; Bahreini, Masoumeh; Sharifmoghaddam, Mohammadreza; Asoodeh, Ahmad

doi:10.1007/s00792-020-01187-9

Cited by 23 publications

(19 citation statements)

References 50 publications

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“…In the presence of DTT there was an increase in its hydrolase activity. This activity was also increased in the presence of metal ions Mn 2+ , Ca 2+ , Na + , and K + 59 . Bacillus pumilus isolated from poultry exhibited high feather degradation.…”

Section: Resultsmentioning

confidence: 86%

Novel recombinant keratin degrading subtilisin like serine alkaline protease from Bacillus cereus isolated from marine hydrothermal vent crabs

Gurunathan

Huang

Ponnusamy

et al. 2021

Sci Rep

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Microbial secondary metabolites from extreme environments like hydrothermal vents are a promising source for industrial applications. In our study the protease gene from Bacillus cereus obtained from shallow marine hydrothermal vents in the East China Sea was cloned, expressed and purified. The protein sequence of 38 kDa protease SLSP-k was retrieved from mass spectrometry and identified as a subtilisin serine proteinase. The novel SLSP-k is a monomeric protein with 38 amino acid signal peptides being active over wide pH (7–11) and temperature (40–80 °C) ranges, with maximal hydrolytic activities at pH 10 and at 50 °C temperature. The hydrolytic activity is stimulated by Ca2+, Co2+, Mn2+, and DTT. It is inhibited by Fe2+, Cd2+, Cu2+, EDTA, and PMSF. The SLSP-k is stable in anionic, non-anionic detergents, and solvents. The ability to degrade keratin in chicken feather and hair indicates that this enzyme is suitable for the degradation of poultry waste without the loss of nutritionally essential amino acids which otherwise are lost in hydrothermal processing. Therefore, the proteinase is efficient in environmental friendly bioconversion of animal waste into fertilizers or value added products such as secondary animal feedstuffs.

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Section: Resultsmentioning

confidence: 86%

Novel recombinant keratin degrading subtilisin like serine alkaline protease from Bacillus cereus isolated from marine hydrothermal vent crabs

Gurunathan

Huang

Ponnusamy

et al. 2021

Sci Rep

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“…The use of enzymes in the manufacture of leather has improved leather quality while reducing environmental pollution. Increased application of alkaline protease at emerging leather industries is due to the elastolytic and keratinolytic activity (Moridshahi et al 2020 ). These influential properties of alkaline protease are very effective in leather processing industries.…”

Section: Microbial Proteases and Its Industrial Applicationsmentioning

confidence: 99%

Microbial proteases: ubiquitous enzymes with innumerable uses

Solanki

Putatunda²,

Bhatia

et al. 2021

3 Biotech

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Proteases are ubiquitous enzymes, having significant physiological roles in both synthesis and degradation. The use of microbial proteases in food fermentation is an age-old process, which is today being successfully employed in other industries with the advent of ‘omics’ era and innovations in genetic and protein engineering approaches. Proteases have found application in industries besides food, like leather, textiles, detergent, waste management, agriculture, animal husbandry, cosmetics, and pharmaceutics. With the rising demands and applications, researchers are exploring various approaches to discover, redesign, or artificially synthesize enzymes with better applicability in the industrial processes. These enzymes offer a sustainable and environmentally safer option, besides possessing economic and commercial value. Various bacterial and fungal proteases are already holding a commercially pivotal role in the industry. The current review summarizes the characteristics and types of proteases, microbial source, their current and prospective applications in various industries, and future challenges. Promoting these biocatalysts will prove significant in betterment of the modern world.

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“…A5.3 from rotten feathers collected on a poultry farm and studied its keratinolytic and proteolytic properties. Bacteria producing keratinases are known to exist [ 27 , 28 , 29 ]; however, the focus of research has been on the isolation, purification, and characterization of properties of individual enzymes.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Bacillus sp. A5.3 Strain with Keratinolytic Activity

Aktayeva

Baltin

Kiribayeva

et al. 2022

Biology

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Environmental safety and economic factors necessitate a search for new ways of processing poultry farm feathers, which are 90% β-keratin and can be used as a cheap source of amino acids and peptones. In this study, feather-decomposing bacteria were isolated from a site of accumulation of rotten feathers and identified as Bacillus. Among them, the Bacillus sp. A5.3 isolate showed the best keratinolytic properties. Scanning electron microscopy indicated that Bacillus sp. A5.3 cells closely adhere to the feather surface while degrading the feather. It was found that Bacillus sp. A5.3 secretes thermostable alkaline proteolytic and keratinolytic enzymes. Zymographic analysis of the enzymatic extract toward bovine serum albumin, casein, gelatin, and β-keratin revealed the presence of proteases and keratinases with molecular weights 20–250 kDa. The proteolytic and keratinolytic enzymes predominantly belong to the serine protease family. Proteome analysis of the secreted proteins by nano-HPLC coupled with Q-TOF mass spectrometry identified 154 proteins, 13 of which are proteases and peptidases. Thus, strain Bacillus sp. A5.3 holds great promise for use in feather-processing technologies and as a source of proteases and keratinases.

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Biochemical characterization of an alkaline surfactant-stable keratinase from a new keratinase producer, Bacillus zhangzhouensis

Cited by 23 publications

References 50 publications

Novel recombinant keratin degrading subtilisin like serine alkaline protease from Bacillus cereus isolated from marine hydrothermal vent crabs

Novel recombinant keratin degrading subtilisin like serine alkaline protease from Bacillus cereus isolated from marine hydrothermal vent crabs

Microbial proteases: ubiquitous enzymes with innumerable uses

Isolation of Bacillus sp. A5.3 Strain with Keratinolytic Activity

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