2007
DOI: 10.1016/j.jmgm.2007.04.006
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Biochemical characterization, homology modeling and docking studies of ornithine δ-aminotransferase—an important enzyme in proline biosynthesis of plants

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Cited by 37 publications
(24 citation statements)
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References 32 publications
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“…In both cases no Michaelis-Menten kinetics were obtained, suggesting a positive cooperative behavior of the enzyme (n 5 1.5 for 2-oxoglutarate and n 5 2.3 for Orn). The measured kinetic values (S 0.5 5 0.8 mM for 2-oxoglutarate and S 0.5 5 6.3 mM for Orn) are in the range of those previously reported (Drejer and Schousboe, 1984;Sekhar et al, 2007). …”
Section: Recombinant Psdoat Protein Exhibits D-oat Activitysupporting
confidence: 84%
“…In both cases no Michaelis-Menten kinetics were obtained, suggesting a positive cooperative behavior of the enzyme (n 5 1.5 for 2-oxoglutarate and n 5 2.3 for Orn). The measured kinetic values (S 0.5 5 0.8 mM for 2-oxoglutarate and S 0.5 5 6.3 mM for Orn) are in the range of those previously reported (Drejer and Schousboe, 1984;Sekhar et al, 2007). …”
Section: Recombinant Psdoat Protein Exhibits D-oat Activitysupporting
confidence: 84%
“…28 As has been mentioned above, docking studies with the mothbean OAT model revealed interactions that are involved in binding of competitive inhibitors. 17 Based on the interaction energies, isoleucine and valine were the best enzyme ligands from this group. Valine showed even more favorable binding energy than ornithine itself.…”
Section: Three-dimensional Structurementioning
confidence: 95%
“…4 Recombinant mothbean OAT was found to be inhibited significantly by isoleucine, valine and serine, whereas proline showed no apparent effect (at a concentration of 1 mM of each). 17 Ten years ago, a cDNA encoding OAT from Arabidopsis thaliana was obtained and sequenced. 8 At present, a number of other plant OAT sequences (e.g., from aquilegia, barrel medic, grape, maize, pine, potato, rice, sorghum and soybean) are available in public DNA and protein databases.…”
Section: Plant Ornithine δ-Aminotransferasesmentioning
confidence: 99%
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“…Proline is synthesized from glutamic acid by Δ1-pyrroline-5-carboxylate (P5C), by two successive reductions catalyzed by the enzymes Δ'-pyrroline-5-carboxylate synthase (P5CS) and Δ'-pyrroline-5-carboxylate reductase (P5CR) (Sekhar et al, 2007).…”
Section: Introductionmentioning
confidence: 99%