Osmotic stress on genetically transformed tobacco plant seeds

Abstract

-Salinity and water deficit limit the productivity of several crops; thus, studies related to the genetic transformation of seeds in a model plant, such as tobacco, can be an alternative to minimize negative impacts caused by environmental conditions. The purpose of this work was to evaluate the tolerance to osmotic stress of seeds from genetically transformed tobacco plants, with the introduction of the proline-synthesizer gene (p5csf129a), under salinity and water deficit conditions. To do so, five events with differences in proline content were selected, ranging from 0.70 to 10.47 µmoles.g -1 of fresh mass. The used saline concentrations were: zero (distilled water); 50; 100; 150 and 200 mmol.L -1 of NaCl, whereas for the water deficit, simulated with PEG 6000, the following osmotic potentials were used: zero (distilled water); -0.2; -0.4; -0.6 and -0.8 MPa. Each tested treatment was evaluated through germination, first germination count and germination speed index tests. It is possible to conclude that seeds from genetically transformed tobacco plants with overexpression of the gene p5csf129a, a proline synthesizer, are more tolerant to osmotic stresses. Tabacco seeds with a proline content of 10.47 µmol.g -1 showed a better perfomance, revealing higher physiological potential.

Ectopic overexpression of mulberry MnT5H2 enhances melatonin production and salt tolerance in tobacco

“…To observe the germination of transgenic tobacco seeds under salt stress, T1 seeds were germinated on filter paper and cotton moistened with salt solution (200mM NaCl), and the germination rate was calculated after 12 days. The NaCl concentration used in the treatment was referred to the previous study (Trombin-Souza et al, 2017;Tok and Temizel, 2021).…”

Biotechnologies Tools for Germination and Preservation of Butia Eriospatha Embryos (Arecaceae)

“…We also observed that embryos under desiccation had an increase in Glu and Gly content by 30 and 6-fold, respectively. Glutamic acid is a precursor of Pro and their high concentration was associated to wild chickpea drought-tolerant seeds (Behboudian et al 2001;Rontein et al 2002;Trombin-Souza et al 2017). Like Glu, the Gly is also indirectly related to desiccation tolerance, since one of the glycinebetaine (GB) synthesis pathways is through glycine Nmethylation Murata 2002, 2011).…”