Biochemical characterization, antimicrobial and hemolytic studies on skin mucus of fresh water spiny eel Mastacembelus armatus

Uthayakumar, Venkatachalam; Ramasubramanian, V.; Senthilkumar, D.; Priyadarisini, Venkatesan Brindha; Harikrishnan, Ramasamy

doi:10.1016/s2221-1691(12)60325-6

Cited by 19 publications

(10 citation statements)

References 23 publications

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“…A similar result was reported in the mucus of two other fish species, Channa punctatus and Cirrhinus mrigala [28]. Also Uthayakumar et al [29] observed that the mucus secretion of Mastacembelus armatus possesses a potent haemolytic activity against sheep and cow blood cells. Previously, Hellio et al [30] reported in the mucus of bony fishes the presence of some antimicrobial agents which bind with microbes and destroy the blood cells, resulting in haemolysis.…”

Section: Discussionsupporting

confidence: 77%

Comparative Study of Antibacterial and Haemolytic Activities in Sea Bass, European Eel and Blackspot Seabream

Caruso¹,

Maricchiolo²,

Genovese

et al. 2014

TOMBJ

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Sea bass (Dicentrarchus labrax, Moronidae), European eel (Anguilla anguilla, Anguillidae) and blackspot seabream (Pagellus bogaraveo, Sparidae) were studied to check their mucus, blood sera and tissue samples for antibacterial and agglutinating activity against a variety of Gram negative and positive bacteria. Samples were also examined for their haemolytic properties against sheep red blood cells. The highest antibacterial activity was detected in the blood sera of blackspot seabream and European eel (against Vibrio alginolyticus) and in the kidneys of sea bass (against Photobacterium damselae subsp. piscicida). Haemolytic properties against sheep red blood cells were observed in the mucus of sea bass and blackspot seabream, as well as in the sera of eel and sea bass. The sera of sea bass and eel showed also agglutinating activity against Pseudomonas aeruginosa and Staphylococcus aureus; the mucus of sea bass was able to agglutinate isolates of Vibrio fluvialis, V. alginolyticus and A. hydrophila. The study suggested that the mucus secretions, biological fluids and organs of the examined fish species can be regarded as an interesting source of bioactive compounds with antibacterial and haemolytic properties.

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Section: Discussionsupporting

confidence: 77%

Comparative Study of Antibacterial and Haemolytic Activities in Sea Bass, European Eel and Blackspot Seabream

Caruso¹,

Maricchiolo²,

Genovese

et al. 2014

TOMBJ

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“…Bragadeeswaran, Priyadharshini, Prabhu, Raj, and Rani (2011) reported that the fish mucus of Cynoglossus arel and Arius caelatus has potential hemolytic activity. Similarly, Uthayakumar et al (2012) also reported hemolytic activity (against sheep and cow blood cells) of mucus extracts of M. armatus.…”

Section: Discussionmentioning

confidence: 80%

“…Earlier, Subramanian et al (2008) also reported low microbial growth inhibition in aqueous fish mucus extracts of a wider range of fish species including Arctic char (Salvelinus alpinus), brook trout (Salvelinus fontinalis), koi carp (Cyprinus carpio), striped bass (Morone saxatilis), haddock (Melanogrammus aeglefinus), and hagfish (Myxine glutinosa) could be due to the presence of low levels of enzymes. In contradiction, this strong inhibitory effect of aqueous mucus extract was reported from various eel fishes Anguilla linnaeus and Mastacembelus armatus (Bragadeeswaran & Thangaraj, 2011;Uthayakumar, Ramasubramanian, Senthilkumar, Brindha, & Harikrishnan, 2012). Hellio et al (2002) reported that fish (Pollachius virens, Labrus bergylta, Scophthalmus rhombus, Platichthys flesus, and Solea solea) mucus extracted with ethanol and DMSO (organic extract) showed strong bactericidal activity against a broad range of pathogens.…”

Section: Discussionmentioning

confidence: 96%

Biotic potential of mucus extracts of giant mudskipper Periophthalmodon schlosseri (Pallas, 1770) from Pichavaram, southeast coast of India

Mahadevan

Mohan

Jothiprakasam

et al. 2019

JoBAZ

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Background: The epidermal mucus of fish contains various bioactive compounds, which play a crucial role in the animal defense mechanisms. However, the mucus from giant mudskipper is not well characterized for its biochemical and pharmacological effects. This study for the first time analyzed the mucus extracts of this species for antimicrobial (against human pathogen) and hemolytic activities. Fourier-transform infrared spectrum analysis (FT-IR) and SDS-PAGE of mucus extracts were also carried out. Results: The protein content in Periophthalmodon schlosseri (Gobiidae: Oxudercine) mucus extracts was 0.66 ± 0.04 mg/ml. The mucus extracts of P. schlosseri showed antibacterial activity against all eight human pathogenic bacteria and four fungal strains tested in this study. The hemolytic activities against chicken and human blood cells were observed with maximum activity for dichloromethane extracts. The FT-IR spectrum of mucus extract of P. schlosseri showed the presence of functional groups, evident protein bands of various molecular weights ranging from 14 kDa to 97 kDa. Conclusion: The obtained results suggested that the mucus of this species has proteinaceous compounds responsible for antimicrobial and hemolytic activities.

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“…[ 37 ] Further, Uthayakumar et al . [ 38 ] observed that the mucus secretion of Mastacembelus armatus possesses a potent hemolytic activity against sheep and cow blood cells.…”

Section: Resultsmentioning

confidence: 99%

Therapeutic significance of Loligo vulgaris (Lamarck, 1798) ink extract: A biomedical approach

2017

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Background:The squid ink extract is well known for its biomedical properties.Objective:In this study, squid Loligo vulgaris was collected from Tuticorin costal water, Bay of Bengal, India.Materials and Methods:Proximate composition of the crude squid ink was studied and found to have protein as the major component over lipid and carbohydrates. Further, bioactive fractions of squid ink were extracted with ethanol, and therapeutic applications such as hemolytic, antioxidant, antimicrobial, and in vitro anti-inflammatory properties were analyzed using standard methods.Results:In hemolytic assay, the squid ink extract exhibited a maximum hemolytic activity of 128 hemolytic unit against tested erythrocytes. In DPPH assay, the ethanolic extract of squid ink has exhibited an antioxidant activity of 83.5%. The squid ink was found to be potent antibacterial agent against the pathogens tested. 200 μL of L. vulgaris ink extract showed remarkable antibacterial activity as zone of inhibition against Escherichia coli (28 mm), Klebsiella pneumoniae (22 mm), Pseudomonas aeruginosa (21 mm), and Staphylococcus aureus (24 mm). The 68.9% inhibition of protein denaturation by the squid ink extract indicated that it has very good in vitro anti-inflammatory properties. The Fourier transform infrared spectroscopy analysis of the ethanolic extracts of the squid ink indicated the presence of functional groups such as 1° and 2° amines, amides, alkynes (terminal), alkenes, aldehydes, nitriles, alkanes, aliphatic amines, carboxylic acids, and alkyl halides, which complements the biochemical background of therapeutic applications.Conclusion:Hence, results of this study concluded that the ethanolic extract of L. vulgaris has many therapeutic applications such as antimicrobial, antioxidant, and anti-inflammatory activities.SUMMARY Squid ink is very high in a number of important nutrients. It’s particularly high in antioxidants for instance, which as well all know help to protect the cells and the heart against damage from free radicals. In the present study, the squid ink have antioxidant, anti-inflammatory and cytotoxic properties and can be considered as promising the developing the drugs. Abbreviations Used: DPPH: 2,2-diphenyl-1-picrylhydrazyl, FTIR: Fourier-transform infrared spectroscopy, BSA: Bovine Serum Albumin

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Biochemical characterization, antimicrobial and hemolytic studies on skin mucus of fresh water spiny eel Mastacembelus armatus

Cited by 19 publications

References 23 publications

Comparative Study of Antibacterial and Haemolytic Activities in Sea Bass, European Eel and Blackspot Seabream

Comparative Study of Antibacterial and Haemolytic Activities in Sea Bass, European Eel and Blackspot Seabream

Biotic potential of mucus extracts of giant mudskipper Periophthalmodon schlosseri (Pallas, 1770) from Pichavaram, southeast coast of India

Therapeutic significance of Loligo vulgaris (Lamarck, 1798) ink extract: A biomedical approach

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