Biochemical characterization and identi cation of enterocins produced by E. hirae And E. faecalis

Abstract

The present study was an endeavour to partially purify and characterize enterocins produced by E.hirae and E.faecalis from UTI patients.Maximum enterococcal isolates were obtained from females of 10-20years age group and compared with males of same age group. Enterocin was partially purifi ed by ammonium sulphate precipitation technique followed by dialysis and analysis by SDS-PAGE revealed molecular mass of enterocin to be 4.5-5.5 kDa approximately .Partially purifi ed enterocin was heat and pH stable and sensitive to proteolytic enzymes, thus proving it to be of proteinaceous nature and was therefore characterized as enterocin. Such bacteriocin have broad fi eld of application including both food industry and medical sector.