Biochemical Characteristics of Staphylococcus Species of Human and Bovine Origin

Langlois, B. E.; Parlindungan, Abdul K.; Harmon, Robert J.; Akers, Katherine

doi:10.4315/0362-028x-53.2.119

Cited by 20 publications

(11 citation statements)

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“…Routine bacteriological tests used in the identification of S. aureus, like mannitol fermentation, DNAse production, VP, etc., are not enough for definitive characterization (7,16). Nevertheless, coagulase production is still the most traditional test used to identify S. aureus (23).…”

Section: Discussionmentioning

confidence: 99%

Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques

Vieira‐da‐Motta¹,

Folly²,

Sakyiama³

2001

Braz. J. Microbiol.

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Contamination of fresh milk with Staphylococcus aureus was assessed comparatively through routine phenotypic (coagulase tube test and coagulase slide test) and genotypic (PCR) screening of 128 S. aureus strains isolated from 555 milk samples. These samples were collected from 362 cows with subclinical mastitis, hosted in different dairy herds at various locations of the Northern and Northeastern rural areas of the State of Rio de Janeiro, 39.7% of which were CMT-positive. All S. aureus isolates tested positive for the presence of the coagulase gene by PCR and the isolates could be grouped into four distinct classes according to the size of the PCR product. The strains also yielded variable results when assayed with coagulase test. Taken together, these data indicate the existence of extensive polymorphism at the coagulase gene locus in the genus Staphylococcus and exemplifies the extent of molecular and phenotypic heterogeneity associated with the strains circulating in rural herds.

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Section: Discussionmentioning

confidence: 99%

Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques

Vieira‐da‐Motta¹,

Folly²,

Sakyiama³

2001

Braz. J. Microbiol.

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“…Hemolysis and colonial morphology were recorded after 24-48 hours. Gram positive cocci with catalase positive and oxidase negative reaction were subjected to coagulase test by tube method (Langlois et al 1990). Other bacterial isolates were examined for their staining affinity, morphological, cultural and their biochemical characters according to the standard methods.…”

Section: Culturingmentioning

confidence: 99%

Acute coliform mastitis in buffaloes (Bubalus bubalis): Clinical findings and treatment outcomes

El-Khodery

Osman

2007

Trop Anim Health Prod

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This report was delineated to study the clinical, bacteriological and therapeutic aspects concerned with acute coliform mastitis in buffaloes. Bacteriological examination of 80 quarter milk samples obtained aseptically from 56 buffaloes with acute mastitis revealed that coliform bacteria was the most common pathogen (45 cases) followed by Staphylococcus aureus (seven cases) then Streptococcus uberis (three cases), and Streptococcus agalactiae (one case). Clinically, hotness, swelling and painful reaction with serous excretion containing clots was recorded in buffaloes with coliform mastitis. The efficacy of ceftiofur was evaluated in the treatment of buffaloes with acute coliform mastitis. Parenteral ceftiofur neither improved clinical signs nor returned milk to pre-infection production level, whereas intramammary ceftiofur and combination of intramammary with parenteral ceftiofur improved the clinical signs in 10/15 and 12/15 buffaloes, respectively. On quarter level, 3/17, 12/17 and 15/21 quarters recovered in groups received parenteral, intramammary and combination therapy, respectively. This study demonstrates that systemic ceftofur is not effective in the treatment of clinical coliform mastitis in buffaloes.

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“…Hemolysis and colonial morphology were recorded after 24 -48 hours. Identification and classification of the isolated bacteria were done based on the colonial and morphological characters [16].…”

Section: Bacteriological Culturingmentioning

confidence: 99%

Prevalence and chemotherapy of contagious skin necrosis in dromedary camels at Qassim region, central of Saudi Arabia

2019

JCH

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This study was executed in Qassim region, central of Saudi Arabia to determine the clinical and therapeutic impact associated with contagious skin necrosis (CSN) in dromedary camels. One thousand dromedary camels were used in this study. The prevalence of contagious skin necrosis among examined camels was 2.70%. The prevalence of the disease was significantly (p = 0.01) higher in camels under three years (4.55%) than camels older than three years (1.49). Concerning gender predisposition, the prevalence was significantly (p = 0.0001) higher in male camels (13.78%) than females ones (1.14%). Clinically, camels infected with contagious skin necrosis showed lesions in the form of multiple circular necrotic areas of the skin and sometimes multiple abscesses in brisket and chest areas. Treatment of the infected camels using Amoxycillin and clavulanic acid gave the same results as penicillin plus streptomycin but it offers a faster cure rate. Finally, it can be concluded that contagious skin necrosis in camels is common in Saudi Arabia and Amoxycillin and clavulanic acid is the best choice for treatment

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Biochemical Characteristics of Staphylococcus Species of Human and Bovine Origin

Cited by 20 publications

References 0 publications

Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques

Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques

Acute coliform mastitis in buffaloes (Bubalus bubalis): Clinical findings and treatment outcomes

Prevalence and chemotherapy of contagious skin necrosis in dromedary camels at Qassim region, central of Saudi Arabia

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