Biochemical characterisation and application of lipases produced by <i><scp>A</scp>spergillus</i> sp. on solid‐state fermentation using three substrates

The aim of this study was to show that abundant and inexpensive plant oils can be biotransformed to increase biological activity (antioxidant, antimicrobial and cytotoxicity) through hydrolysis reaction catalysed by lipases. We tested homemade and commercial lipases through the biotransformation of nine different plant oils in forty different combinations. First, the chemical composition of the samples was investigated. Thereafter, biological tests were conducted to assess the antioxidant and antimicrobial activity of the sampled biotransformation products, as well as analyzed their influence on the viability of healthy and cancer cells. Summarising, sunflower, corn and olive oils modified by orange waste-obtained lipases presented the most promising results, reaching up to 90% of antioxidant activity increase and significant growth inhibition of bacteria colonies belonging to genera Escherichia, Listeria, Staphylococcus, Pseudomonas and Salmonella. In addition, those compounds affected human oral squamous carcinoma cells. The bioconversion of plant oils through lipases improves their biological properties and might be an option for biotechnological application.Bioconversion into biological activity V. C. B. Francisco et al.

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“…Acids titration was the instrument chosen to check reaction efficiency – hydrolysis rate calculations followed the method by Fleuri et al . ().…”

Section: Methodsmentioning

confidence: 97%

Plant oil bioconversion into increase biological activity through lipases derived from wastes

Francisco

Okino‐Delgado

Zanutto-Elgui

et al. 2018

Int J of Food Sci Tech

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“…Under SSF, fungi have proven that they are able to produce a myriad of enzymes with different applications, including detergent manufacturing, food processing, textile and pharmaceutical industries, medical therapy, molecular biology, bioremediation, and biological control. 17 The enzymatic complexes known as cellulases are capable of degrading lignocellulosic residues, this ability making them highly desirable for industry use worldwide, in ethanol production, the treatment of waste papers, fruit-juice extraction, single-cell protein, cotton processing, pulp bleaching, and animal feed additives. 18 Using lignocellulosic wastes in SSF induces the production of fungal cellulases.…”

Section: Introductionmentioning

confidence: 99%

Improved lignocellulolytic enzyme production and antioxidant extraction using solid‐state fermentation of olive pomace mixed with winery waste

Filipe¹,

Fernandes²,

Castro³

et al. 2019

Biofuels Bioprod Bioref

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Olive pomace is characterized by its low nutritional value and high phenolic content, which hinders its direct use as animal feed, fertilizer, or as a substrate in bioprocesses such as solid-state fermentation (SSF). A possible strategy for bioprocessing olive pomace by SSF is the mixture of olive mill wastes with other wastes produced in the same region, such as winery wastes. This may improve the production of bioactive compounds like enzymes and antioxidant phenolics. A simplex-centroid design was used to evaluate the use of olive mill and winery wastes alone or in combination as a substrate for SSF with Aspergillus niger and Aspergillus ibericus. Synergistic effects of combinations of crude olive pomace (COP), exhausted olive pomace (EOP), vine trimming shoots (VTS), and exhausted grape marc (EGM) were observed in the production of xylanases, cellulases, β-glucosidases, and in the variation in total phenolics and antioxidant activity of SFF extracts. A multiple response optimization was carried out, leading to the following optimal mixture of substrates: for A. niger, 23% (w/w) COP, 30% EGM, 33% VTS, 14% EOP; for A. ibericus, 30% EGM, 36% VTS, 34% EOP. The scale-up to tray bioreactor with optimal substrate made it possible to achieve the maximum xylanase, cellulase, and β-glucosidase production of 189.1 ± 26.7, 56.3 ± 2.1 and 10.9 ± 0.8 U/g, respectively. The antioxidant activity of fermented wastes was also improved 2.2-fold as compared with unfermented wastes. Thus, a combination of olive mill and winery wastes in SSF is a potential strategy to increase their value and to develop a circular strategy in these industries.

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“…Numerous filamentous fungi species produce these enzymes, especially those from the genus Aspergillus (Contesini et al, 2010;Fleuri et al, 2014). The genus Aspergillus is currently considered as one of the largest biotechnological substance producers, with several species found in nature (Liu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…There are about 837 (Hawksworth et al, 2011) Aspergillus species commonly isolated from soil and decaying plants. These fungi produce a number of extracellular enzymes (among them: lipases), many of which are applied in different industry sectors (Contesini et al, 2010;Fleuri et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Despite functional electrical stimulation (FES) advantages, this technology is still not widely used for lipase production in large scale. This fact is due to the lack of systematic studies at bench scale, for example, the definition of microorganisms producing suitable substrates and culture conditions (Holker and Lenz, 2005;Fleuri et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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High-level lipase production by Aspergillus candidus URM 5611 under solid state fermentation (SSF) using waste from Siagrus coronata (Martius) Becari

Farias¹,

Souza²,

Sousa³

et al. 2015

Afr. J. Biotechnol.

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The current study evaluated lipase production by Aspergillus candidus URM 5611 through solid state fermentation (SSF) by using almond bran licuri as a new substrate. The microorganism produced high levels of the enzyme (395.105 U gds -1 ), thus surpassing those previously reported in the literature. The variable moisture content, the inductor concentration, temperature and pH were analyzed. The best production conditions were initial moisture content of 75%, 0% of inductor, at 25°C, pH 5.5, in 10.0 g of substrate. The variables initial moisture and inductor contents showed significant effects on lipase production by increasing enzyme activity in about 200% when compared with the initial screening (197.44 U gds -1 ). Optimum lipase activity was obtained at pH 2.5 at an optimum temperature of 65°C. The lipase was stable from pH 2.5 to 9.0 and at temperatures between 30 to 65°C. Results from the study are promising for the economic use and value addition from these important agro residues, which are abundantly available in Brazil. This is the first report on lipase production by A. candidus URM 5611 reaching a much higher yield of enzyme under SSF with almond bran licuri used as substrate.

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Biochemical characterisation and application of lipases produced by Aspergillus sp. on solid‐state fermentation using three substrates

Cited by 19 publications

References 45 publications

Plant oil bioconversion into increase biological activity through lipases derived from wastes

Plant oil bioconversion into increase biological activity through lipases derived from wastes

Improved lignocellulolytic enzyme production and antioxidant extraction using solid‐state fermentation of olive pomace mixed with winery waste

High-level lipase production by Aspergillus candidus URM 5611 under solid state fermentation (SSF) using waste from Siagrus coronata (Martius) Becari

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