Biochemical changes in the cytoplasm of bovine oocytes during the in vitro maturation process: a Raman microscopy study

Jimenez, Luis Emanuel; Juárez, Ana Carolina; Roldán‐Olarte, Mariela; Alvarez, Rosa Maria Susana

doi:10.1007/s11259-021-09882-4

Cited by 3 publications

(1 citation statement)

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“…This technique may be used as a screening tool for selection of oocytes for fertilization. Jimenez et al ( 29 ) measured the biochemical changes in the cytoplasm of bovine oocytes during the in vitro maturation process using Raman microscopy. The markers for proteins, lipids, and carbohydrates were used to investigate the ooplasm at four stages.…”

Section: Raman Spectroscopymentioning

confidence: 99%

Application of imaging and spectroscopy techniques for grading of bovine embryos - a review

Shivaani,

Madan

2024

Front. Vet. Sci.

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Although embryo transfers have grown considerably in the cattle industry, the selection of embryos required for successful pregnancies remains a challenging task. Visual inspection of 7th-day embryos using a stereomicroscope, followed by classification based on morphological features is the most commonly practiced procedure. However, there are inaccuracies and inconsistencies in the manual grading of bovine embryos. The objective of this review was to evaluate the potential of imaging and spectroscopic techniques in the selection of bovine embryos. Digital analysis of microscopic images through extracting visual features in the embryo region, and classification using machine learning methods have yielded about 88–96% success in pregnancies. The Raman spectral pattern provides valuable information regarding developmental stages and quality of the embryo. The Raman spectroscopy approach has also been successfully used to determine various parameters of bovine oocytes. Besides, Fourier Transform Infrared (FTIR) spectroscopy has the ability to assess embryo quality through analyzing embryo composition, including nucleic acid and amides present. Hyperspectral Imaging has also been used to characterize metabolite production during embryo growth. Although the time-lapse imaging approach is beneficial for morphokinetics evaluation of embryo development, optimized protocols are required for successful implementation in bovine embryo transfers. Most imaging and spectroscopic findings are still only at an experimental stage. Further research is warranted to improve the repeatability and practicality to implement in commercial facilities.

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Section: Raman Spectroscopymentioning

confidence: 99%