The role of glucose and fructose on lipid droplet metabolism in human normal bronchial and cancer lung cells by Raman spectroscopy

Kopec, Monika; Beton-Mysur, Karolina

doi:10.1016/j.chemphyslip.2023.105375

Cited by 3 publications

(3 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All Raman spectra presented in the manuscript were normalized in Origin by using a model divided by norm. [60][61][62] The normalization, model: divided by norm (divide the spectrum by the dataset norm) was performed by using Origin software according to the formula:…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biochemical changes in lipid and protein metabolism caused by mannose-Raman spectroscopy studies

Kopeć,

Beton-Mysur,

Abramczyk

2024

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…All Raman spectra presented in the manuscript were normalized in Origin by using a model divided by norm. 60–62…”

Section: Methodsmentioning

confidence: 99%

Biochemical changes in lipid and protein metabolism caused by mannose-Raman spectroscopy studies

Kopeć,

Beton-Mysur,

Abramczyk

2024

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

“…The obtained Raman spectra and all imaging data were analyzed using cluster analysis (CA), which was executed using the WITec Project Plus package (for the removal of cosmic rays and smoothing and background corrections). More details about the equipment, settings, and parameters can be found in previous works [66,67,[86][87][88].…”

Section: Raman Imagingmentioning

confidence: 99%

Raman Imaging—A Valuable Tool for Tracking Fatty Acid Metabolism—Normal and Cancer Human Colon Single-Cell Study

Beton-Mysur,

Kopec,

Brozek-Pluska

2024

IJMS

Self Cite

View full text Add to dashboard Cite

Altered metabolism of lipids is a key factor in many diseases including cancer. Therefore, investigations into the impact of unsaturated and saturated fatty acids (FAs) on human body homeostasis are crucial for understanding the development of lifestyle diseases. In this paper, we focus on the impact of palmitic (PA), linoleic (LA), and eicosapentaenoic (EPA) acids on human colon normal (CCD-18 Co) and cancer (Caco-2) single cells using Raman imaging and spectroscopy. The label-free nature of Raman imaging allowed us to evaluate FAs dynamics without modifying endogenous cellular metabolism. Thanks to the ability of Raman imaging to visualize single-cell substructures, we have analyzed the changes in chemical composition of endoplasmic reticulum (ER), mitochondria, lipid droplets (LDs), and nucleus upon FA supplementation. Analysis of Raman band intensity ratios typical for lipids, proteins, and nucleic acids (I1656/I1444, I1444/I1256, I1444/I750, I1304/I1256) proved that, using Raman mapping, we can observe the metabolic pathways of FAs in ER, which is responsible for the uptake of exogenous FAs, de novo synthesis, elongation, and desaturation of FAs, in mitochondria responsible for energy production via FA oxidation, in LDs specialized in cellular fat storage, and in the nucleus, where FAs are transported via fatty-acid-binding proteins, biomarkers of human colon cancerogenesis. Analysis for membranes showed that the uptake of FAs effectively changed the chemical composition of this organelle, and the strongest effect was noticed for LA. The spectroscopy studies have been completed using XTT tests, which showed that the addition of LA or EPA for Caco-2 cells decreases their viability with a stronger effect observed for LA and the opposite effect observed for PA. For normal cells, CCD-18 Co supplementation using LA or EPA stimulated cells for growing, while PA had the opposite impact.

show abstract

Metabolism changes caused by glucose in normal and cancer human brain cell lines by Raman imaging and chemometric methods

Kopec,

Beton-Mysur,

Surmacki

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Glucose is the main source of energy for the human brain. This paper presents a non-invasive technique to study metabolic changes caused by glucose in human brain cell lines. In this paper we present the spectroscopic characterization of human normal brain (NHA; astrocytes) and human cancer brain (CRL-1718; astrocytoma and U-87 MG; glioblastoma) control cell lines and cell lines upon supplementation with glucose. Based on Raman techniques we have identified biomarkers that can monitor metabolic changes in lipid droplets, mitochondria and nucleus caused by glucose. We have studied the vibrations at 750 cm −1 , 1444 cm −1 , 1584 cm −1 and 1656 cm −1 as a function of malignancy grade. We have compared the concentration of cytochrome, lipids and proteins in the grade of cancer aggressiveness in normal and cancer human brain cell lines. Chemometric analysis has shown that control normal, control cancer brain cell lines and normal and cancer cell lines after supplementation with glucose can be distinguished based on their unique vibrational properties. PLSDA (Partial Least Squares Discriminant Analysis) and ANOVA tests have confirmed the main role of cytochromes, proteins and lipids in differentiation of control human brain cells and cells upon supplementation with glucose. We have shown that Raman techniques combined with chemometric analysis provide additional insight to monitor the biology of astrocytes, astrocytoma and glioblastoma after glucose supplementation.

show abstract

The role of glucose and fructose on lipid droplet metabolism in human normal bronchial and cancer lung cells by Raman spectroscopy

Cited by 3 publications

References 61 publications

Biochemical changes in lipid and protein metabolism caused by mannose-Raman spectroscopy studies

Biochemical changes in lipid and protein metabolism caused by mannose-Raman spectroscopy studies

Raman Imaging—A Valuable Tool for Tracking Fatty Acid Metabolism—Normal and Cancer Human Colon Single-Cell Study

Metabolism changes caused by glucose in normal and cancer human brain cell lines by Raman imaging and chemometric methods

Contact Info

Product

Resources

About