Biochemical Changes in Secondary Metabolites in Wounded and Deteriorated Cassava Roots

Tanaka, Yoshiyuki; Data, Emma S.; Hirose, Shohei; Taniguchi, Takeshi; Uritani, Ikuzô

doi:10.1080/00021369.1983.10865717

Cited by 24 publications

(11 citation statements)

References 4 publications

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“…The preliminary analysis by HPLC shows that in vitro quillay ethanolic extracts contain a series of secondary metabolites (Table 1), which have been reported as antifungal compounds on phytopathogenic fungi, such as saponins (Oleszek et al 1990;Osbourn et al 1996;Apablaza et al 2002;Chapagain et al 2007), and the phenolic compounds chlorogenic, caffeic, vanillic, and salicylic acids (Derek et al 1989;Harborne and Baxter 1993;NitaLazar et al 2004;Prats et al 2006), and the coumarin scopoletin (Uritani and Hoshiya 1953;Minamikawa et al 1992Minamikawa et al , 1964Murray et al 1982;Tanaka et al 1983;Gutiérrez et al 1995;Prats et al 2006). These results suggest that the antifungal activity of in vitro quillay culture could be explained, at least partly, by the presence of saponinas and phenolic compounds.…”

Section: Resultsmentioning

confidence: 96%

Effect of extracts from in vitro-grown shoots of Quillaja saponaria Mol. on Botrytis cinerea Pers.

Ribera

Cotoras

Zúñiga

2008

World J Microbiol Biotechnol

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The ethanolic and aqueous extracts from in vitro shoots of Quillaja saponaria Mol. (Quillay) were studied for their antifungal activity against the phytopathogenic fungus Botrytis cinerea Pers. These extracts reduced conidial germination and mycelial growth of B. cinerea, ethanolic extracts being more active than aqueous extracts. In addition, the damage areas produced by this fungus on tomato leaves and strawberry fruits pretreated with quillay extracts were diminished. The fungitoxic effect of in vitro-grown quillay extract was similar to those obtained with commercial fungicides of both natural (BC-1000) and synthetic (iprodione-dicarboximide) origin. On the other hand, the antifungal action of quillay extracts obtained from adult trees naturally grown was only slightly superior to the fungitoxic activity of the extract from in vitro plants. HPLC analysis of the extract showed that it contained saponins and some phenolic compounds such as chlorogenic, caffeic, vanillic, and salicylic acids, and scopoletin, which have been identified as antifungal agents on phytopathogenic fungi. The results obtained in this work, suggests that extracts of in vitro-grown quillay have an important protective effect against B. cinerea and support the use of an in vitro culture system as a biotechnological alternative to obtain environmental safe antifungal quillay extracts to control B. cinerea, contributing to the preservation of this indigenous Chilean species.

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Section: Resultsmentioning

confidence: 96%

Effect of extracts from in vitro-grown shoots of Quillaja saponaria Mol. on Botrytis cinerea Pers.

Ribera

Cotoras

Zúñiga

2008

World J Microbiol Biotechnol

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“…3) and, due to the 150-200-fold increase of scopoletin concentration, it is assumed that scopoletin is involved in the post-harvest physiological deterioration (PPD) of cassava roots (Tanaka et al, 1983;Wheatley and Schwabe, 1985;Buschmann et al, 2000b). Scopoletin accumulation tends to be more pronounced in cassava cultivars with high susceptibility to deterioration; then, after 6 days, the scopoletin concentration is less pronounced due to the metabolism of scopoletin to an insoluble blue-black coloured product (the probable cause of vascular streaking during PPD) by means of a peroxidase (Reilly et al, 2003).…”

Section: Hydroxycoumarinsmentioning

confidence: 99%

Cassava: An appraisal of its phytochemistry and its biotechnological prospects

et al. 2010

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“…Increased respiration (Uritani and Reyes 1984;Hirose 1986;Uritani 1998), changes in lipid composition (Lalaguna and Agudo 1989), synthesis of ethene (Hirose et al 1984), accumulation of secondary metabolites from the phenylpropanoid pathway, and increases in many enzyme activities such as PAL and chalcone synthase, glucanase, chitinase, proteinase inhibitors, HRGPs, invertase, catalase, dehydrogenase, peroxidase and polyphenol oxidase (Rickard 1981;Tanaka et al 1983) have been reported during the development of PPD. In addition to detecting PAL and polyphenol oxidase (PPO) activity, cytological analyses of the wound surface in cassava roots showed the presence of phenols, lipids, carbohydrates, and lignins (Rickard 1981).…”

Section: Introductionmentioning

confidence: 96%

Study of the Early Events Leading to Cassava Root Postharvest Deterioration

Iyer

Mattinson

Fellman

2010

Tropical Plant Biol.

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Cassava (Manihot esculenta Crantz) roots, the fourth most important food crop of the world, is the major carbohydrate source for more than 600 million people in Africa, parts of Latin America, Oceania, and Asia. Besides being a rich source of starch (∼80% of root), the root is also rich in vitamin C, some carotenoids, calcium, and potassium. Upon harvest, roots begin a process of physiological decay within 24-36 h called postharvest physiological deterioration or PPD. The early events leading to PPD are not known. Research to date concerning the study of PPD has mostly focused on the signaling events several hours after harvest. Upon examination of physiological and biochemical changes occurring 3 or 4 h after cassava root detachment, changes in the nature and type of volatile compounds emitted, secondary metabolites accumulated, and changes in the expression of key genes in reactive oxygen species (ROS) turnover were observed along with a correspondent increase in tissue cytoplasmic singlet oxygen presence using radical-specific fluorescent imaging of tissue samples. It is likely that these findings have significant implications to help us understand and assist in dissection of the early events leading to the postharvest deterioration of cassava root.

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Biochemical Changes in Secondary Metabolites in Wounded and Deteriorated Cassava Roots

Cited by 24 publications

References 4 publications

Effect of extracts from in vitro-grown shoots of Quillaja saponaria Mol. on Botrytis cinerea Pers.

Effect of extracts from in vitro-grown shoots of Quillaja saponaria Mol. on Botrytis cinerea Pers.

Cassava: An appraisal of its phytochemistry and its biotechnological prospects

Study of the Early Events Leading to Cassava Root Postharvest Deterioration

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