Biochemical changes in Oenothera biennis plants infected by ‘Candidatus Phytoplasma solani’

Kiprovski, Biljana; Đalović, Ivica; Adamovic, Dusan; Mitrović, Petar; Marjanović-Jeromela, Ana; Malenčić, Đorđe; Popović, Tatjana

doi:10.1007/s42161-018-0068-0

Cited by 9 publications

(13 citation statements)

References 26 publications

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“…A previous study reported that the leaf tissue of O. biennis plants was more vulnerable to the oxidative stress induced by Ca. Phytoplasma solani compared to the root [8]. Similarly, here, we present that leaves of carrots infected by Ca.…”

Section: Discussionsupporting

confidence: 75%

“…While roots of symptomatic plants accumulated carbohydrates by 25% and had a constitutively high amount of carotenoids, no changes were found in carbohydrate content in the infected leaves, as it has been reported for other species [8]. The activation of the phenylpropanoid pathway by a phytoplasma that results in differential accumulation of hydroxycinnamates, flavonoids, and anthocyanins has been shown for various species [7,8,35]. Results implied that tested leaves compared to the root of the carrot was preferentially exposed to the oxidative stress induced by Ca.…”

Section: Discussionsupporting

confidence: 57%

“…Based on the data on the regulation of phenylalanine ammonia-lyase activity (PAL) accompanied by an accumulation of hydroxycinnamates and inhibition of flavonoid biosynthesis in phytoplasma-infected plants, Musetti [7] suggested that polyphenols are involved in plant defense against phytoplasma. In our previous study, similar changes in the pattern of inducible polyphenols in Oenothera biennis plants were obtained when infected by Candidatus Phytoplasma solani [8]. SA plays a crucial role in numerous plant defense responses, such as local and systemic pathogen-induced defense gene activation, the oxidative burst, and pathogen-induced cell death [9][10][11][12][13].…”

Section: Introductionsupporting

confidence: 61%

“…Despite a much lower accumulation of total polyphenols and GSH (10%), the oxidative effect on lipids in roots was less pronounced (18%) compared to the leaf (61%). While roots of symptomatic plants accumulated carbohydrates by 25% and had a constitutively high amount of carotenoids, no changes were found in carbohydrate content in the infected leaves, as it has been reported for other species [ 8 ]. The activation of the phenylpropanoid pathway by a phytoplasma that results in differential accumulation of hydroxycinnamates, flavonoids, and anthocyanins has been shown for various species [ 7 , 8 , 35 ].…”

Section: Discussionmentioning

confidence: 64%

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Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani

Mitrović

Djalović

Kiprovski

et al. 2021

Plants

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The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the “stolbur” group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionsupporting

confidence: 57%

Section: Introductionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 64%

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Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani

Mitrović

Djalović

Kiprovski

et al. 2021

Plants

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“…P. solani' and recorded in Vojvodina as hosts of stolbur phytoplasma: parsley, carrot, tobacco, evening primrose (Oenothera biennis) etc. (Mitrović et al, 2013;Kiprovski et al, 2018;Mitrović et al, 2021). Celery (Apium graveolens L.) is biannual herbaceous plant belonging to the parsley family (fam.…”

mentioning

confidence: 99%

Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog

Popović¹,

Mitrović²,

Kosovac³

2021

Ratar i povrt

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'Candidates Phytoplasma solani', known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during August 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for 'Ca. P. solani' presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stoll 1 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmpl. Combining molecular tools PCR/RFLP and sequencing reviled two 'Ca. P. solani' multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/ Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the 'Ca. P. solani' diversity on celery, but supplemented with genotyping of the vmpl gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of 'Ca. P. solani' affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.

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Physiological responses of grapevine (Vitis vinifera var. ‘Pinot gris’) affected by different flavescence dorée genotypes: dynamics through the development of phytoplasma infection

Davosir,

Šola,

Šeruga Musić

2024

J Plant Dis Prot

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Phytoplasmas are phytopathogenic bacteria that cause serious damage to agriculture. A quarantine pathogen avescence dorée phytoplasma (FDp), often associated with grapevine yellows disease, affects viticultural production across Europe. However, the mechanisms of FDp pathogenicity still are not elucidated. In this study, symptomatic and asymptomatic grapevine (Vitis vinifera L. var. 'Pinot gris') were sampled. Two different FDp genotypes (M38 and M54) were identi ed, and genotype-dependent changes to grapevine physiological responses through the development of FDp infection were analysed.Correlation analyses established a potential linked between measured physiological parameters and relative FDp DNA abundance. Increased malondialdehyde levels pointed to the oxidative stress in infected leaves, and highly correlated with the activation of L-ascorbic acid synthesis. Levels of hydrogen peroxide were reduced in infected leaves, possibly as FDp mechanism to avoid plant-derived oxidative damage.Genotype M54 was associated with a lower accumulation of soluble sugars and lower damage to photosynthetic pigments, while retaining a higher titre than M38. Therefore, pronounced phytoplasma genotype-dependent changes in grapevine physiology, potentially caused by the differences between M54 and M38 on the level of the e ciency of their effectors should be further investigated. Altogether, results provide data on certain targets of FDp in grapevine and could assist the identi cation of potential speci c effectors of this phytoplasma to aid the efforts of FDp management in European vineyards.

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Biochemical changes in Oenothera biennis plants infected by ‘Candidatus Phytoplasma solani’

Cited by 9 publications

References 26 publications

Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani

Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani

Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog

Physiological responses of grapevine (Vitis vinifera var. ‘Pinot gris’) affected by different flavescence dorée genotypes: dynamics through the development of phytoplasma infection

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