Biochemical changes in barley plants after excessive supply of copper and manganese

Demirevska-Kepova, K.; Simova‐Stoilova, Lyudmila; Stoyanova, Z.; Hölzer, Regina; Feller, Urs

doi:10.1016/j.envexpbot.2004.02.004

Cited by 332 publications

(153 citation statements)

References 65 publications

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“…It has been reported previously that proline accumulation in stress tolerant plants is higher than in stress sensitive plants [56]. AsA is the major primary antioxidant in all sub-cellular compartments, which reacts directly with ROS, and also acts as a secondary antioxidant by reducing the oxidized form of α-tocopherol and preventing membrane damage [57]. In this study, the AsA content was significantly higher (Figure 2(d)) in cultivar K6 than in cultivar K9 in response to Pb-stress.…”

Section: Discussionmentioning

confidence: 99%

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars

Nareshkumar¹,

Veeranagamallaiah²,

Pandurangaiah³

et al. 2015

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Lead (Pb) is an important environmental pollutant extremely toxic to plants and other living organisms including humans. To assess Pb phytotoxicity, a pot culture experiment was carried out using two groundnut cultivars (Arachis hypogaea L. cultivar K6 and cultivar K9) on plant growth, ROS levels, lipid peroxidation, and antioxidant metabolism using biochemical, histochemical methods. Plants were grown in pots for 14 days, in the botanic garden, and subjected to Pb-stress (0, 100, 200, 400 and 800 ppm) by adding Pb (NO3)2 solution and further allowed to grow for 10 days. The results showed that cultivar K6 registered lower Pb accumulation than cultivar K9, however, localization of Pb was greater in roots than leaves in both groundnut cultivars. The Pb-stress results in an increase in free radicals (O2• − and H2O2) generation in both groundnut cultivars, but more significantly in cultivar K9 than K6. Pb-stress also caused significant changes in the rate of peroxidation as shown in the levels of malondialdehyde (MDA) content in roots and leaves of both groundnut cultivars. Free proline, ascorbic acid (AsA) and non-protein thiol (NP-SH) contents were increased in cultivar K6 due to Pb-stress, but less in cultivar K9. Pb treated plants showed increased levels of antioxidant enzymes such as superoxide dismutase (SOD), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) glutathione reductase (GR) and glutathione Stransferase (GST). Isozyme band intensities of SOD, GPX and APX were more consistent with the respective changes in antioxidative enzyme activities. These results indicate that cultivar K6 possesses greater tolerance potential for Pb toxicity than cultivar K9.

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Section: Discussionmentioning

confidence: 99%

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars

Nareshkumar¹,

Veeranagamallaiah²,

Pandurangaiah³

et al. 2015

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“…Copper excess also interferes in the activity of several enzymes (Lombardi and Sebastiani, 2005) and in some aspects associated with photosynthesis, pigment synthesis, fatty acid and protein metabolism, respiration, N fixation processes and membrane integrity, among others (Ducić and Polle, 2005). Some chloroplast proteins and the enzymes glutamine synthase (GS) and ferredoxin-dependent glutamate synthase (Fd-GOGAT), involved in the assimilation of NH 4 + , are very susceptible to heavy metal toxicity, especially Fd-GOGAT to Cu excess (Demirevska-Kepova et al, 2004). Probably its most important effect is associated with the impairment of the photosynthetic electron transport system, promoting the production of radicals that initiate the reactions of the peroxidative chain, involving membrane lipids (Ducić and Polle, 2005).…”

Section: Coppermentioning

confidence: 99%

Tolerance and prospection of phytoremediator woody species of Cd, Pb, Cu and Cr

Almeida

Valle

Mielke

et al. 2007

Braz. J. Plant Physiol.

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High concentrations of Cd, Pb, Cu and Cr can cause harmful effects to the environment. These highly toxic pollutants constitute a risk for aquatic and terrestrial life. They are associated with diverse bioavailable geochemical fractions, like the water-soluble fraction and the exchangeable fraction, and non-available fractions like those associated with the crystalline net of clays and silica minerals. Depending upon their chemical and physical properties we can distinguish different mechanisms of metal toxicity in plants, such as production of reactive oxygen species from auto-oxidation, blocking and/or displacement of essential functional groups or metallic ions of biomolecules, changes in the permeability of cellular membranes, reactions of sulphydryl groups with cations, affinity for reactions with phosphate groups and active groups of ADP or ATP, substitution of essential ions, induction of chromosomal anomalies and decrease of the cellular division rate. However, some plant species have developed tolerance or resistance to these metals naturally. Such evolution of ecotypes is a classic example of local adaptation and microevolution, restricted to species with appropriate genetic variability. Phytoremediator woody species, with (i) high biomass production, (ii) a deep root system, (iii) high growth rate, (iv) high capacity to grow in impoverished soils, and (v) high capacity to allocate metals in the trunk, can be an alternative for the recovery of degraded soils due to excess of metallic elements. Phytoremediation using woody species presents advantageous characteristics as an economic and ecologically viable system, making it an appropriate, practical and successful technology. Key-words: decontamination, heavy metals, hyperaccumulation, phytodegradation, phytostabilization, phytotoxicity Tolerância e prospecção de espécies lenhosas fitorremediadoras de Cd, Pb, Cu e Cr: Altas concentrações de Cd, Pb, Cu e Cr podem provocar efeitos danosos ao ambiente. Esses poluentes altamente tóxicos constituem um risco para a vida aquática e terrestre. Encontram-se associados às diversas frações geoquímicas biodisponíveis, a exemplo da fração solúvel em água e da fração trocável, e as não disponíveis como a fração associada à rede cristalina de argilas e de minerais silicatados. Conforme as suas propriedades químicas e físicas podem-se distinguir mecanismos diferentes de toxicidade de metais em plantas, tais como produção de espécies reativas de oxigênio pela auto-oxidação, bloqueio e, ou, deslocamento de grupos funcionais ou de íons metálicos essenciais de biomoléculas, mudanças na permeabilidade de membranas celulares, reações de grupos sulfidrílicos com cátions, afinidade para reações com grupos fosfatos e grupos ativos de ADP ou ATP, substituição de íons essenciais, indução de anomalias cromossomais e decréscimo da taxa de divisão celular. Várias espécies vegetais desenvolveram, naturalmente, tolerância ou resistência a esses metais. Essa evolução de ecótipos é exemplo clássico de adaptação local e de microevolução,...

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“…Heavy metals present at elevated levels may cause an increased production of reactive oxygen species (ROS) in plant cells on one hand [42][43][44][45][46][47][48][49][50][51] and may be involved in the enzymatic detoxification of ROS on the other hand [2,3,42,[52][53][54]. Stresses (e.g., drought, heat and high light intensity) may also cause an accumulation of ROS and of ROS-damaged cell constituents [54,55].…”

Section: Heavy Metals: Micronutrients or Pollutants?mentioning

confidence: 99%

Heavy Metals in Crop Plants: Transport and Redistribution Processes on the Whole Plant Level

2015

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Abstract:Copper, zinc, manganese, iron, nickel and molybdenum are essential micronutrients for plants. However, when present in excess they may damage the plant or decrease the quality of harvested plant products. Some other heavy metals such as cadmium, lead or mercury are not needed by plants and represent pollutants. The uptake into the roots, the loading into the xylem, the acropetal transport to the shoot with the transpiration stream and the further redistribution in the phloem are crucial for the distribution in aerial plant parts. This review is focused on long-distance transport of heavy metals via xylem and phloem and on interactions between the two transport systems. Phloem transport is the basis for the redistribution within the shoot and for the accumulation in fruits and seeds. Solutes may be transferred from the xylem to the phloem (e.g., in the small bundles in stems of cereals, in minor leaf veins). Nickel is highly phloem-mobile and directed to expanding plant parts. Zinc and to a lesser degree also cadmium are also mobile in the phloem and accumulate in meristems (root tips, shoot apex, axillary buds). Iron and manganese are characterized by poor phloem mobility and are retained in older leaves.

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Biochemical changes in barley plants after excessive supply of copper and manganese

Cited by 332 publications

References 65 publications

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars

Tolerance and prospection of phytoremediator woody species of Cd, Pb, Cu and Cr

Heavy Metals in Crop Plants: Transport and Redistribution Processes on the Whole Plant Level

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Biochemical changes in barley plants after excessive supply of copper and manganese

Cited by 332 publications

References 65 publications

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (&lt;i&gt;Arachis hypogaea&lt;/i&gt; L.) Cultivars

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (&lt;i&gt;Arachis hypogaea&lt;/i&gt; L.) Cultivars

Tolerance and prospection of phytoremediator woody species of Cd, Pb, Cu and Cr

Heavy Metals in Crop Plants: Transport and Redistribution Processes on the Whole Plant Level

Contact Info

Product

Resources

About

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars