Biochemical changes associated with embryonic and larval development in the American lobster Homarus americanus Milne Edwards

Sasaki, Glenn C.

doi:10.1575/1912/3142

Cited by 38 publications

(41 citation statements)

References 165 publications

(318 reference statements)

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“…Starting the next morning, larvae were fed frozen brine shrimp three times daily and received an additional feeding of a fatty acid supplement. The supplement, described in Sasaki (1984), contained two essential fatty acids (20: 6u.J 3 and 22: 6w3) found to be missing from the frozen brine shrimp (Sasaki, 1984).…”

Section: Larval Culturementioning

confidence: 99%

“…Larval stage and molt stage of larval lobsters sampled for this study were determined using the method of Sasaki (1984) which involves assessment of the amount of tissue withdrawal from the tail of the larvae. Only molt stage C animals from each stage were sampled for hemolymph.…”

Section: Larval Culturementioning

confidence: 99%

“…bcalculated from Sill value, presuming heart size as proportional to ashfree dry weight (from Sasaki, 1984 Morris, 1990;Burnett and Infantino, 1984 ). Effectors often shift the P0 2 , but can change cooperativity as well (e.g., lactate alters both properties).…”

Section: ·;__ __mentioning

confidence: 99%

“…The larvae were scooped from the mother's tank and placed in plankton kriesels supplied with flow-through seawater (T = 18-21 o C, S = 28 % ) The larvae were kept under a varying light-dark regime with at least 8 hours darkness per day. The larvae were reared on a diet of frozen brine shrimp plus a fatty acid supplement (Sasaki, 1984) containing two essential fatty acids (20:6 3 and 22:6 3 fatty acids) missing from the shrimp. Larvae were batch-reared in kriesels until stage IV, at which time they were separated into individual trays that floated on the surface of a large tank.…”

Section: Larval Culturementioning

confidence: 99%

“…Larvae were removed from the plankton kriesels, then sorted by stage and molt staged using the method of Sasaki (1984). Only intermolt (molt stage C) animals were sampled for the studies conducted here.…”

Section: Sample Collectionmentioning

confidence: 99%

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Developmental changes in the structure and function of lobster hemocyanin

Olson¹

1991

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Section: Larval Culturementioning

confidence: 99%

Section: Larval Culturementioning

confidence: 99%

Section: ·;__ __mentioning

confidence: 99%

Section: Larval Culturementioning

confidence: 99%

Section: Sample Collectionmentioning

confidence: 99%

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Developmental changes in the structure and function of lobster hemocyanin

Olson¹

1991

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Aspects of the embryology and neural development of the American lobster

et al. 1992

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It is feasible to study the anatomical, physiological, and biochemical properties of identifiable neurons in lobster embryos. To exploit fully the advantages of this preparation and to lay the foundation for single-cell studies, our recent goals have been to 1) establish a quantitative staging system for embryos, 2) document in detail the lobster's embryonic development, 3) determine when uniquely identifiable neurons first acquire their transmitter phenotypes, and 4) identify particular neurons that may serve developmental functions. Behavioral, anatomical, morphometric, and immunocytochemical studies have led to a detailed characterization of the growth and maturation of lobster embryos and to the adoption of a percent-staging system based upon the eye index of Perkins (Fish. Bull., 70:95-99, 1972). It is clear from these studies that the lobster nauplius molts at approximately 12% embryonic development (E12%) into a metanauplius, which subsequently undergoes a complete molt cycle within the egg. This molt cycle climaxes with the emergence of the first-stage larva shortly after hatching. Serotonin and proctolin, neurohormones widely distributed in the lobster nervous system, appear at different times in development. Serotonin immunoreactive neurons begin to appear at approximately E10%, with the adult complement being established by E50%. In contrast, proctolin immunoreactive neurons appear later and attain their full complement over a protracted period including larval and juvenile stages. The development of serotonergic deutocerebral neurons and their targets, the olfactory and accessory lobes in the brain, are also examined. The olfactory lobes are forming by E10% and have acquired their glomerular organization by E50%, whereas the formation of the accessory lobes is delayed; the early rudiments of the accessory lobes are seen by E50%, and glomeruli do not form until the second larval stage.

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