Biochemical, cellular and molecular aspects of Cymbidium orchids: an ecological and economic overview

Balilashaki, Khosro; Vahedi, Maryam; Ho, Thanh-Tam; Niu, Shan-Ce; Cardoso, Jean Carlos; Zotz, Gerhard; Khodamzadeh, Amir Ali

doi:10.1007/s11738-022-03359-w

Cited by 6 publications

(5 citation statements)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They are commercially valuable for their therapeutic virtues and attractive blossoms. Orchids are also of ecological importance due to their relationship with specialized pollinators and microorganisms for seed germination ( Givnish et al., 2015 ; Balilashaki et al., 2022 ). Rhynchostylis retusa (L.) Blume is an epiphytic orchid listed as endangered and native to tropical Asia, particularly Assam and Arunachal Pradesh.…”

Section: Introductionmentioning

confidence: 99%

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

et al. 2022

View full text Add to dashboard Cite

Root-associated bacteria strongly affect plant growth and development by synthesizing growth regulators and stress-relieving metabolites. The present study is mainly focused on assessing aerial root-associated bacteria of Rhynchostylis retusa (L.) Blume is an endemic epiphytic orchid responsible for auxin production and influencing plant growth. A bacterial isolate, Microbacterium testaceum Y411, was found to be the most active producer of indole-3-acetic acid (IAA). The maximum IAA production (170µg/mL) was recorded with the bacterium at optimum process parameters such as pH 7, temperature 30°C, and tryptophan 1000 µg/mL in a culture medium for 48 h. The extracted auxin was purified and analyzed by FT-IR, HPLC, and HR-MS, indicating bacterial auxin has a similar mass value to 4-chloroindole-3-acetic acid auxin. Furthermore, the bacterial auxin was tested on in vitro propagation of orchid, Cymbidium aloifolium, and 90% seed germination was recorded in Murashige and Skoog’s medium supplemented with bacterial auxin. The novel results obtained in this study are used for agricultural applications and the Microbacterium testaceum Y411 is a valuable biotechnological resource for a natural auxin.

show abstract

Section: Introductionmentioning

confidence: 99%

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

et al. 2022

View full text Add to dashboard Cite

show abstract

“…TDZ is distinct due to its lack of the purine ring, which is typically found in other cytokinins. TDZ has been employed to promote callus formation and somatic embryogenesis (Balilashaki et al, 2022;Dewir et al, 2018;Fritsche et al, 2022). Furthermore, the role that TDZ plays in facilitating adventitious shoot formation across various plant species has been established, surpassing the adenine-type cytokinins.…”

Section: Discussionmentioning

confidence: 99%

The Comprehensive In Vitro Propagation and Genetic Homogeneity Analysis of Cryptocoryne crispatula var. yunnanensis: Addressing the Conservation Concerns for an Endangered Species in China and the Mekong Basin

Sakulsathaporn,

Mapanao

2024

Preprint

View full text Add to dashboard Cite

This study focused on the propagation of the endangered aquatic plant species Cryptocoryne crispatula var. yunnanensis, threatened by hydropower dam construction in Thailand and classified as endangered in China. The objective was to propagate the species using shoot explants. A sterilization method with a 45.45% success rate involved treating shoots with 0.01% HgCl2 for 90 minutes and 5% commercial bleach (6% NaOCl) for 20 minutes. The study investigated the effect of Murashige and Skoog (MS) medium, supplemented with 0.5 mg/L NAA and 1-4 mg/L cytokinins (BA, kinetin, and TDZ), on shoot initiation and proliferation. TDZ was found to be more effective than BA and kinetin in enhancing shoot growth. The optimal shoot induction, averaging 7.14 shoots per explant, occurred in MS medium with 0.5 mg/L NAA and 3 mg/L TDZ. A medium of 0.5 mg/L NAA and 1 mg/L TDZ significantly increased shoot proliferation, yielding an average of 23.75 shoots per explant. The most successful ex vitro rooting and acclimatization method involved 1X vitamin stock MS medium with 0.5 mg/L IBA, followed by transfer to plastic pots with a 1:1 sand and vermiculite mix, achieving a 73.33% survival rate and an average of 6.31 roots per explant. Genetic uniformity and stability of the propagated clones were verified using ISSR markers. This protocol enhances the conservation efforts for C. crispatulavar. yunnanensis by supporting its multiplication and preservation in synthetic habitats.

show abstract

“…Orchids possess significant economic and ornamental significance. The ornamental value of orchids is significantly influenced by the growth and development of their organs, including the development of floral organs [26][27][28]. However, it takes several years from seed to flowering and they must go through five stages: seed germination, rhizome growth, leaf-bud differentiation, plant growth, and flowering.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the WUSCHEL-Related Homeobox (WOX) Gene Family in Three Cymbidium Species and Expression Patterns in C. goeringii

Wang,

Chen,

Cheng

et al. 2024

Horticulturae

View full text Add to dashboard Cite

Numerous members of the WOX gene family play pivotal roles during the processes of growth and development in many plants, as has been demonstrated. Cymbidium goeringii, Cymbidium ensifolium, and Cymbidium sinense are ornamental plants with a fascinating floral morphology that are economically important in China. However, there is limited knowledge about the members of the WOX gene family and their functions in these three Cymbidium species. Hence, the WOX genes in three Cymbidium species were identified on the ground of the genomes data of C. goeringii, C. ensifolium, and C. sinense in this study. These identified WOX genes were further studied for their physicochemical properties, evolutionary relationship, gene structure, protein structure, and cis-acting elements of promoters, as well as the expression pattern of the WOX genes in different tissues of C. goeringii. The findings revealed that eight WOX genes in C. goeringii, twelve WOX genes in C. ensifolium, and nine WOX genes were identified. These WOX genes were further subdivided into WUS, ancient, and intermediate clades. The length of the coding region ranged from 149 to 335 aa, and it was predicted that all WOX genes would be located on the cell nucleus. The promoter cis-acting elements primarily comprised stress response, phytohormone response, plant growth and development, and transcription factor elements. Furthermore, both the transcriptomic data and RT-qPCR analysis showed that most WOX genes may be involved in multiple developmental stages of C. goeringii. To sum up, these results may serve as a theoretical foundation for further study of the function analysis of WOX genes in orchids.

show abstract

Biochemical, cellular and molecular aspects of Cymbidium orchids: an ecological and economic overview

Cited by 6 publications

References 135 publications

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

The Comprehensive In Vitro Propagation and Genetic Homogeneity Analysis of Cryptocoryne crispatula var. yunnanensis: Addressing the Conservation Concerns for an Endangered Species in China and the Mekong Basin

Genome-Wide Identification of the WUSCHEL-Related Homeobox (WOX) Gene Family in Three Cymbidium Species and Expression Patterns in C. goeringii

Contact Info

Product

Resources

About