Biochemical and pharmacological study of venom of the wolf spider Lycosa singoriensis

Zh, Liu; Qian, Weikang; Li, J; Zhang, Yiya; Liang, Songping

doi:10.1590/s1678-91992009000100008

Cited by 17 publications

(13 citation statements)

References 25 publications

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“…These results consistent with that of Ahmed et al (2012) which found that scorpion Heterometrus xanopus has antibacterial effect on B. subtillis with inhibition zone of 30 mm, while, Escherichia coli showed resistance against the same venom. This study was compatible with Liu et al (2009) study on wolf spider Lycosa singoriensis which was effective only on B. subtillis under very low concentration (3 mg/ml) and has weak effect on C. albicans fungus. Interestingly, Buthus martensii venom was detected only against Grampositive bacteria but not against Gram-negative one (Gao et al, 2007).…”

Section: Discussionsupporting

confidence: 84%

“…Valavi and Alemzadeh-Ansari (2008), reported that Hemiscorpius lepturus scorpion has hemolytic activities. Also, crude venom of wolf spider Lycosa singoriensis has hemolytic effect on human erythrocytes in a dose-dependent manner (Liu et al, 2009). On the other hand, some toxins in both scorpion venom as hadrurin and in centipedes as scolopendrin I showed hemolysis activity at different concentrations, and it reached 75% hemolysis under the effect of hadrurin from scorpion (Wenhua et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

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Investigation of the antimicrobial and hemolytic activity of venom of some Egyptian scorpion

Salama¹,

Geasa²

2014

J. Microbiol. Antimicrob.

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Leuirus quinquestriatus, Androctonus amoreuxi and Androctonus australis are venomous scorpion in Egypt. Their venom is complex mixture of salts, neurotoxins, peptides and proteins which has therapeutic applications, and can rapidly kill a broad range of microbes. Estimations of total proteins of the venom indicated that L. quinquestriatus had the highest value than the others (10.64 ± 0.04). Hemolytic activity of human erythrocytes was detected and showed that all concentrations (20, 8 and 5 mg/ml) of tested scorpion species crude venom have hemolytic activity against human erythrocytes. The present study was conducted to evaluate the antimicrobial activity of the three Egyptian scorpion's venom against four Gram-positive and negative bacterial strains (Bacillus cereus, Bacillus subtillis, Citrobacter freundi and Klibsella pneumonia) in addition to one fungus species Candida albicans. The results show that L. quinquestriatus venom has a significant antibacterial effect against B. subtillis and C. freundi. In contrast, A. amoreuxi and A. australis venoms do not have a noticeable effect on tested microbes. So, the aim of the present study was to investigate the antimicrobial activity of crude venom against Gram-negative, as well as Gram-positive bacteria, fungi and to evaluate the hemolytic activity of the three investigated species on human erythrocytes.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Investigation of the antimicrobial and hemolytic activity of venom of some Egyptian scorpion

Salama¹,

Geasa²

2014

J. Microbiol. Antimicrob.

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“…In conclusion, 2DE evidently acts as the better tool in profiling proteins found in a complex mixture such as those of snake venoms in comparison to SDS-PAGE, though the latter remains vital as a rapid and inexpensive investigating technique (30). Two-dimensional gel electrophoresis, due to its additional parameter of separation is more suitable for detecting minute differences between closely related samples.…”

Section: Discussionmentioning

confidence: 99%

Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis

Vejayan¹,

Yee²,

Ponnudurai³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Snake venoms comprise a highly complex mixture of proteins, which requires for their characterization the use of versatile two-dimensional electrophoresis techniques. In the present study, venoms obtained from eight snakes (Ophiophagus hannah, Naja kaouthia, Naja sumatrana, Bungarus fasciatus, Trimeresurus sumatranus, Tropidolaemus wagleri, Enhydrina schistosa and Calloselasma rhodostoma) commonly found in Malaysia were separated based on two independent properties, isoelectric point (pI) and molecular weight (MW). Many differences in snake venoms at the inter-family, inter-subfamily, inter-genus and inter-species levels were revealed. Notably, proteins from individuals of the Viperidae family -Trimeresurus sumatranus, Tropidolaemus wagleri and Calloselasma rhodostoma -were found to be numerous and scattered by the two-dimensional gel electrophoresis (2DE) specifically in regions between 37 and 100 kDa compared to the Elapidae venom proteins. The latter were clustered at the basic and lower molecular mass region (less than 20 kDa). Trains of spots were commonly observed, indicating that these proteins may be derived from post-translational modifications. Ophiophagus hannah (Elapidae) revealed a great amount of protein spots in the higher molecular mass range when compared to Enhydrina schistosa, Naja kaouthia, Naja sumatrana and Bungarus fasciatus. Overall 2DE showed large differences in the venom profile of each species, which might be employed as an ancillary tool to the identification of venomous snake species.

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“…These peptides display an amphipathic a-helix, typical of many very active AMPs, and show activity against Gram-negative bacteria (Escherichia coli) and yeast (Candida glabrata). AMPs from L. singoriensis venom (Xu et al 1989;Budnik et al 2004;Liu et al 2009) with molecular masses in the range of 2,000-3,000 Da have also been isolated.…”

Section: Introductionmentioning

confidence: 99%

LyeTx I, a potent antimicrobial peptide from the venom of the spider Lycosa erythrognatha

et al. 2009

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LyeTx I, an antimicrobial peptide isolated from the venom of Lycosa erythrognatha, known as wolf spider, has been synthesised and its structural profile studied by using the CD and NMR techniques. LyeTx I has shown to be active against bacteria (Escherichia coli and Staphylococcus aureus) and fungi (Candida krusei and Cryptococcus neoformans) and able to alter the permeabilisation of L: -alpha-phosphatidylcholine-liposomes (POPC) in a dose-dependent manner. In POPC containing cholesterol or ergosterol, permeabilisation has either decreased about five times or remained unchanged, respectively. These results, along with the observed low haemolytic activity, indicated that antimicrobial membranes, rather than vertebrate membranes seem to be the preferential targets. However, the complexity of biological membranes compared to liposomes must be taken in account. Besides, other membrane components, such as proteins and even specific lipids, cannot be discarded to be important to the preferential action of the LyeTx I to the tested microorganisms. The secondary structure of LyeTx I shows a small random-coil region at the N-terminus followed by an alpha-helix that reached the amidated C-terminus, which might favour the peptide-membrane interaction. The high activity against bacteria together with the moderate activity against fungi and the low haemolytic activity have indicated LyeTx I as a good prototype for developing new antibiotic peptides.

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Biochemical and pharmacological study of venom of the wolf spider Lycosa singoriensis

Cited by 17 publications

References 25 publications

Investigation of the antimicrobial and hemolytic activity of venom of some Egyptian scorpion

Investigation of the antimicrobial and hemolytic activity of venom of some Egyptian scorpion

Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis

LyeTx I, a potent antimicrobial peptide from the venom of the spider Lycosa erythrognatha

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