Biochemical and molecular study on the beneficial effect of Solubilized coenzyme Q10 on thioacetamide-induced liver fibrosis in adult male rats

Abstract

The present work aimed to evaluate the combined therapeutic effect of both solubilized Coenzyme Q10 and Silymarin on thioacetamide (TAA) induced liver injury. A total of thirty adult male albino rats, weighing 200±10 g were allocated into five groups. Hepatic injury was induced by Thioacetamide (TAA) 20 mg/ kg b wt for 28 days by intraperitoneal injection twice a week. On the other hand, solubilized Coenzyme Q10 (20 mg/kg) & Silymarin (50 mg/kg) were taken orally for 28 days. Upon using the aspartate and platelet ratio index (APRI) & aspartate aminotransferase-alanine aminotransferase ratio (AAR), the TAA group recording the highest values while treatments using Coenzyme Q10 and or Silymarin could decrease theirs values near the control one. However, solubilized CoQ10 could attenuate liver injury induced by TAA via balanced oxidative stress symbols (GSH, GSSG), NO level, and down-regulation gene expression of fibrotic markers TGF-β, collagen-1α, and, TIMP1 as well as enhanced the expression for MMP2 and cytochrome P 450 (CYP 2E1& CYP 3A2). Collectively, solubilized CoQ10 singly or combined with Silymarin showed higher therapeutic impacts more than or equivalents to Silymarin singly treatment for liver fibrosis induced by TAA.