Biochemical and molecular heterogeneity among isolates of Yersinia ruckeri from rainbow trout (Oncorhynchus mykiss, Walbaum) in north west Germany

Huang, Yidan; Runge, Martin; Michael, Geovana Brenner; Schwarz, Štefan; Jung, Arne; Steinhagen, Dieter

doi:10.1186/1746-6148-9-215

Cited by 29 publications

(23 citation statements)

References 38 publications

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“…Farms with a trading relationship (Huang et al 2013) to help trace the dissemination route of the Y. ruckeri isolates during recurrent disease outbreaks. This characterisation scheme revealed that in the majority of the farms, which were repeatedly sampled, bacteria from different TPs were found, and on several occasions, bacteria of different TPs were simultaneously present in the same farm.…”

Section: Discussionmentioning

confidence: 99%

“…During this field study, a total of 48 Y. ruckeri isolates were obtained from 16 of the 27 typing groups (TP) described by Huang et al (2013) (Table 3). The TPs were defined according to results obtained from biochemical (API 20 E) and molecular biological typing methods (repetitive PCR and PFGE macrorestriction analysis).…”

Section: Distribution Of Yersinia Ruckeri Isolatesmentioning

confidence: 99%

“…Kidney and spleen samples were collected from trout showing clinical signs and inoculated into tryptic soy agar. Y. ruckeri were isolated and identified as described previously (Huang et al 2013). Briefly, the isolates were examined by Gram staining and were biochemically characterised by API 20E tests (bioMérieux).…”

Section: Isolation Of Bacteriamentioning

confidence: 99%

“…In addition, repetitive sequence-based PCRs were performed, including BOX-A1R-based repetitive extragenic palindromic-PCR (BOX-PCR), (GTG) 5 -PCR, enterobacterial repetitive intergenic consensus (ERIC) PCR and repetitive extragenic palindromic (REP) PCR, as described by Amann (2007). Moreover, pulsed-field gel electrophoresis (PFGE) of Not I-digested genomic DNA of the Y. ruckeri isolates was performed as described previously (Huang et al 2013). Subsequently, the detailed molecular and phenotypical characterisation scheme based on the API 20E profiles and the patterns received by PFGE macrorestriction analysis and the above mentioned repetitive sequence-based PCRs (Huang et al 2013) was applied to investigate the persistence of Y. ruckeri isolates in the farms and their dissemination between farms.…”

Section: Isolation Of Bacteriamentioning

confidence: 99%

“…obs). In order to investigate the occurrence and further spreading of these 'vaccine-tolerant' isolates, Y. ruckeri strains isolated from trout farms in NRW were analysed with the molecular and phenotypic characterisation scheme for Y. ruckeri developed by Huang et al (2013).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Yersinia ruckeri strains isolated from trout farms in northwest Germany

Huang¹,

Jung²,

Schäfer³

et al. 2015

Dis. Aquat. Org.

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Enteric redmouth disease (ERM), caused by Yersinia ruckeri, is among the most important infectious diseases in rainbow trout Oncorhynchus mykiss aquaculture in Europe. Our aim was to analyse the persistence of Y. ruckeri strains in trout farms in northwest Germany and their dissemination between farms based on a detailed molecular and phenotypical characterisation scheme. The data on identification and characterisation of Y. ruckeri strains and examining the distribution of these strains in the field could serve as a basis for preventive disease monitoring plans. During the observation period from June 2011 until June 2012, we collected 48 Y. ruckeri isolates from 12 different rainbow trout hatcheries. In total, 44 (91.7%) of the isolates were nonmotile; in particular, all isolates recovered during the sampling period in winter and early spring were non-motile. In several trout farms, characteristic farm-specific Y. ruckeri isolates from particular typing groups were isolated throughout the year, while in other farms, which had a trading relationship between each other, ERM outbreaks were caused by Y. ruckeri from the same typing group. Our data indicate that in some farms, the causative Y. ruckeri strains persisted in the respective trout farm. The presence of Y. ruckeri from the same typing group in farms with a trading relationship indicates a dissemination of the infection between the farms. KEY WORDS: Epidemiology · Enteric redmouth disease · Rainbow trout · Oncorhynchus mykissResale or republication not permitted without written consent of the publisher FREE REE ACCESS CCESS

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Section: Discussionmentioning

confidence: 99%

Section: Distribution Of Yersinia Ruckeri Isolatesmentioning

confidence: 99%

Section: Isolation Of Bacteriamentioning

confidence: 99%

Section: Isolation Of Bacteriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of Yersinia ruckeri strains isolated from trout farms in northwest Germany

Huang¹,

Jung²,

Schäfer³

et al. 2015

Dis. Aquat. Org.

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Use of proteomic-based MALDI-TOF mass spectra for identification of bacterial pathogens in aquaculture: a review

Çağatay

2024

Aquacult Int

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The fisheries and aquaculture sectors are growing rapidly, reflecting their importance in meeting the ever-increasing human population’s demands for animal protein. Production progress in this sector, however, is challenging as a result of increased deaths from epidemics caused by bacterial infectious diseases in aquaculturally important species. In order to minimize the impact of such diseases, quick and reliable diagnosis of pathogens, timely intervention, and control of the disease are essential to ensure the health of aquaculture and fisheries stocks. Thus, high-throughput proteomics-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used as a rapid, accurate, and species-specific tool for the identification of bacterial fish pathogens and antibiotic resistance. The aim of this article is to review and evaluate the results of nearly three hundred papers published from 2010 to 2023 on the application of MALDI-TOF MS in aquaculture, showing that this method has been increasingly used over the years for the species-level identification and antibiotic resistance of fifty different gram-positive and negative bacterial aquatic pathogens encountered in wild or cultured fish, shellfish, invertebrates, and crustaceans. In addition, the history and general principles of the MALDI-TOF MS method are also mentioned in the article so that fish disease researchers interested in the use of this technology can see all aspects of the topic.

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Comparison of molecular and biochemical heterogeneity of Yersinia ruckeri strains isolated from Turkey and the USA

2016

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Biochemical and molecular heterogeneity among isolates of Yersinia ruckeri from rainbow trout (Oncorhynchus mykiss, Walbaum) in north west Germany

Cited by 29 publications

References 38 publications

Analysis of Yersinia ruckeri strains isolated from trout farms in northwest Germany

Analysis of Yersinia ruckeri strains isolated from trout farms in northwest Germany

Use of proteomic-based MALDI-TOF mass spectra for identification of bacterial pathogens in aquaculture: a review

Comparison of molecular and biochemical heterogeneity of Yersinia ruckeri strains isolated from Turkey and the USA

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