Biochemical and metabolic characteristics of articular cartilage from osteonecrotic human femoral heads

Hj, Mankin; Thrasher, AZ; Hall, D.

doi:10.2106/00004623-197759060-00002

Cited by 22 publications

(6 citation statements)

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“…The fixed biopsies were embedded in paraffin, sectioned with a thickness of ϳ7 m onto glass slides, stained with Alcian blue and H&E, and graded according to Mankin. 21 The histological Mankin score is the standard grading scheme used for cartilage and incorporates grading based on structure fissuring, cell cloning, loss of proteoglycan, and tidemark integrity, where grade 0 represents normal cartilage and grade 14 represents severely degenerated cartilage. Two investigators evaluated randomized and blind-coded samples independently, and the final Mankin score was calculated as a mean of the two evaluations.…”

Section: Methodsmentioning

confidence: 99%

A Chemometric Analysis for Evaluation of Early-Stage Cartilage Degradation by Infrared Fiber-Optic Probe Spectroscopy

Thomson

DiCarlo

et al. 2005

Appl Spectrosc

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In vivo identification of early-stage cartilage degradation could positively impact disease progression in osteoarthritis, but to date remains a challenge. The primary goal of this study was to develop an infrared fiber-optic probe (IFOP) chemometric method using partial least squares (PLS1) to objectively determine the degree of cartilage degradation. Arthritic human tibial plateaus (N = 61) were obtained during knee replacement surgery and analyzed by IFOP. IFOP data were collected from multiple regions of each specimen and the cartilage graded according to the Collins Visual Grading Scale of 0, 1, 2, or 3. These grades correspond to cartilage morphology that displayed normal, swelling or softening, superficially slight fibrillation, and deeper fibrillation or serious fibrillation, respectively. The model focused on detecting early cartilage degradation and therefore utilized data from grades 0, 1, and 2. The best PLS1 calibration utilized the spectral range 1733-984 cm(-1), and independent validation of the model utilizing 206 spectra to create a model and 105 independent test spectra resulted in a correlation between the predicted and actual Collins grade of R2 = 0.8228 with a standard error of prediction of 0.258 with a PLS1 rank of 15 PLS factors. A preliminary PLS1 calibration that utilized a cross-validation technique to investigate the possibility of correlation with histological tissue grade (33 spectra from 18 tissues) resulted in R2 = 0.8408 using only eight PLS factors, a very encouraging outcome. Thus, the groundwork for use of IFOP-based chemometric determination of early cartilage degradation has been established.

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Section: Methodsmentioning

confidence: 99%

A Chemometric Analysis for Evaluation of Early-Stage Cartilage Degradation by Infrared Fiber-Optic Probe Spectroscopy

Thomson

DiCarlo

et al. 2005

Appl Spectrosc

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“…The histology sections from human tibial plateau samples were stained with Alcian Blue and H&E and graded according to Mankin et al 33,34 . The histological Mankin score is the standard grading scheme used for cartilage and incorporates grading based on structure fissuring, cell cloning, loss of PG and tidemark integrity, where grade 0 represents normal cartilage and grade 14 represents severely degenerated cartilage.…”

Section: Cartilage Tissuesmentioning

confidence: 99%

A novel method for determination of collagen orientation in cartilage by Fourier transform infrared imaging spectroscopy (FT-IRIS)

Doty

et al. 2005

Osteoarthritis and Cartilage

164

163

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Thicknesses of each zone of normal equine cartilage (calculated based on differences in collagen orientation) were equivalent as determined by PLM and FT-IRIS. Comparable outcomes were obtained from the PLM and FT-IRIS analyses of repair and osteoarthritis tissues, whereby similar zonal variations in collagen orientation were apparent for the two methods. However, the PLM images of human osteoarthritic cartilage showed less obvious zonal discrimination and orientation compared to the FT-IRIS images, possibly attributable to the FT-IRIS method detecting molecular orientation changes prior to their manifestation at the microscopic level.

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“…Our observation that label-ing did occur, when 3H-Tdr that had high specific activity was used for a long (19-hour) incubation period, suggests that some of these cells already are engaged in or are more readily induced to enter into DNA synthesis than is ordinarily perceived. Several recent publications (26,27) report that low-level incorporation of thymidine into articular cartilage of aged rabbits and humans can be detected by counting scintillations. Minor degenerative changes are common in the joints in older age groups.…”

Section: Resultsmentioning

confidence: 99%

Stimulation of dna synthesis by ascorbate in cultures of articular chondrocytes

Krystal

Morris

Sokoloff

1982

Arthritis & Rheumatism

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The addition of 0.2 mM Na L-ascorbate increased the incorporation of 3H-thymidine by rabbit articular chondrocytes in cell and organ culture. The stimulatory response of explants to ascorbate was potentiated by pretreatment of the cartilage with 0.2% clostridial collagenase (type 1) or trypsin for 15-30 minutes. In explants there was a latent period of 3 to 4 days before increased labeling of the nuclei could be detected. The effect was transient and declined after 8 days of culture. it was more evident in organ cultures of immature (3-month-old) than 2-to 3-year-old rabbits. Age differences were not detected in cell cultures. Explants of adult human articular cartilage were stimulated by ascorbate when the medium was supplemented with 10% fresh human serum but not by fetal bovine serum. The findings indicated that synthesis of DNA by articular chondrocytes in situ is regulated by responsiveness of the cells proper to compounds such as vitamin C, by properties of the extracellular matrix, and by factors in the serum. Ascorbate was cytotoxic at concentrations >0.2 mM in the presence of certain batches of serum and when added to monolayers before the cells attached to the surface of the flask.The principal metabolic action of vitamin C on articular chondrocytes that has been studied is the stimulation of collagen synthesis (1). There also have been indications of effects on cartilage matrix (2,3) and on several catabolic enzyme activities (4). Less is known about the effect of L-ascorbic acid on DNA synthesis. In a previous study (3, outgrowth of chondrocytes from explants from rabbit articular cartilage was shown to be enhanced by this compound. So, too, was proliferation of chondrocytes in monolayer culture (6). We report here that ascorbate, over a limited range of concentrations, promoted DNA synthesis by articular chondrocytes in organ-cultured cartilage as well as in cell culture. The significance is that articular chondrocytes, traditionally considered terminally differentiated and incapable of mitotic division in situ, can, in fact, engage in replicative activity while still surrounded by an extracellular matrix. MATERIALS AND METHODSChondrocytes were cultured variously as monolayers or tissue explants from the joints of rabbits that ranged in age from 3 months to more than 2 years (Table I , Figure 1).Monolayer culture. The methods for cell culture have been described in detail elsewhere (7-9). Chondrocytes were dissociated from the cartilage by serial enzymatic digestion, the definitive step using 0.2% weight/volume (w/v) clostridial collagenase (Worthington-Millipore CLS-11). The experiments were performed on cells in their first passage. Dulbecco-Vogt modified Eagle medium (DMEM) containing 450 mg glucose/dl supplemented by 10% fetal bovine serum and penicillin-streptomycin (all from GIBCO) was used. Except where otherwise indicated, the final concentration of

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Biochemical and metabolic characteristics of articular cartilage from osteonecrotic human femoral heads

Cited by 22 publications

References 0 publications

A Chemometric Analysis for Evaluation of Early-Stage Cartilage Degradation by Infrared Fiber-Optic Probe Spectroscopy

A Chemometric Analysis for Evaluation of Early-Stage Cartilage Degradation by Infrared Fiber-Optic Probe Spectroscopy

A novel method for determination of collagen orientation in cartilage by Fourier transform infrared imaging spectroscopy (FT-IRIS)

Stimulation of dna synthesis by ascorbate in cultures of articular chondrocytes

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