Biochemical and genomic characterization of a novel bacteriophage BUCT555 lysing Stenotrophomonas maltophilia

Han, Pengjun; Hu, Yunjia; An, Xiaoping; Song, Lihua; Fan, Huahao; Tong, Yigang

doi:10.1016/j.virusres.2021.198465

Cited by 19 publications

(13 citation statements)

References 53 publications

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“…All strains were cultivated in Luria–Bertani (LB) medium at 37°C. Stenotrophomonas maltophilia isolate 118 (SMA118) was used as a host to isolate phage from sewage samples as the previously described ( Han et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

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Characterization of the Bacteriophage BUCT603 and Therapeutic Potential Evaluation Against Drug-Resistant Stenotrophomonas maltophilia in a Mouse Model

Han

Zhang

et al. 2022

Front. Microbiol.

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Stenotrophomonas maltophilia (S. maltophilia) is a common opportunistic pathogen that is resistant to many antibiotics. Bacteriophages are considered to be an effective alternative to antibiotics for the treatment of drug-resistant bacterial infections. In this study, we isolated and characterized a phage, BUCT603, infecting drug-resistant S. maltophilia. Genome sequencing showed BUCT603 genome was composed of 44,912 bp (32.5% G + C content) with 64 predicted open reading frames (ORFs), whereas no virulence-related genes, antibiotic-resistant genes or tRNA were identified. Whole-genome alignments showed BUCT603 shared 1% homology with other phages in the National Center for Biotechnology Information (NCBI) database, and a phylogenetic analysis indicated BUCT603 can be classified as a new member of the Siphoviridae family. Bacteriophage BUCT603 infected 10 of 15 S. maltophilia and used the TonB protein as an adsorption receptor. BUCT603 also inhibited the growth of the host bacterium within 1 h in vitro and effectively increased the survival rate of infected mice in a mouse model. These findings suggest that bacteriophage BUCT603 has potential for development as a candidate treatment of S. maltophilia infection.

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Section: Methodsmentioning

confidence: 99%

“…An adsorption assay was performed according to the protocol described before ( Han et al, 2021 ). The exponentially growing host strain SMA118 (2.0 × 10 8 CFU/ml) was co-cultured with the phage suspensions (MOI = 0.1) at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the Bacteriophage BUCT603 and Therapeutic Potential Evaluation Against Drug-Resistant Stenotrophomonas maltophilia in a Mouse Model

Han

Zhang

et al. 2022

Front. Microbiol.

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“…Phenol-chloroform method was used for the DNA extraction of phage IME268 as previously described. 22 Briefly, phage suspension was treated with nucleases DNase (1 mg/mL) and RNase (1mg/mL) for overnight at 37°C. After that Zncl 2 was served for phage precipitation and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Genomic Analysis of a Novel Drexlervirial Bacteriophage IME268 with Lytic Activity Against Klebsiella pneumoniae

Nazir¹,

Qi²,

Shi³

et al. 2022

IDR

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Introduction Klebsiella pneumoniae , a multidrug resistant bacterium, that causes nosocomial infections including septicemia, pneumonia etc. Bacteriophages are potential antimicrobial agents for the treatment of antibiotic resistant bacteria. Methods and Results In this study, a novel bacteriophage IME268 was isolated from hospital sewage against clinical multi-drug resistant Klebsiella pneumoniae . Transmission electron microscopy and genomic characterization of this phage exhibited it belongs to the Webervirus genus, Drexlerviridae family. Phage IME268 possessed a double-stranded DNA genome composed of 49,552bp with a GC content of 50.5%. The phage genome encodes 77 open reading frames, out of 44 are hypothetical proteins while 33 had assigned putative functions. No tRNA, virulence related or antibiotic resistance genes were found in phage genome. Comparative genomic analysis showed that phage IME268 has 95% identity with 87% query cover with other phages in NCBI database. Multiplicity of infection, one step growth curve and host range of phage were also measured. Conclusion According to findings, Phage IME268 is a promising biological agent that infects Klebsiella pneumoniae and can be used in future phage therapies.

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“…The purification of phage was performed three times by taking a single plaque with the double-layer agar method ( 4 ). The phage crude extract was concentrated by ultracentrifugation, and highly purified virus particles were obtained by sucrose density gradient centrifugation ( 5 ). The phage particles were observed with a JEM-1200EX transmission electron microscope (JEOL Ltd., Tokyo, Japan) at an accelerating voltage of 80 kV.…”

Section: Announcementmentioning

confidence: 99%

Genomic Analysis of Bacteriophage BUCT86 Infecting Klebsiella Pneumoniae

Han

Zhu

et al. 2022

Microbiol Resour Announc

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Biochemical and genomic characterization of a novel bacteriophage BUCT555 lysing Stenotrophomonas maltophilia

Cited by 19 publications

References 53 publications

Characterization of the Bacteriophage BUCT603 and Therapeutic Potential Evaluation Against Drug-Resistant Stenotrophomonas maltophilia in a Mouse Model

Characterization of the Bacteriophage BUCT603 and Therapeutic Potential Evaluation Against Drug-Resistant Stenotrophomonas maltophilia in a Mouse Model

Characterization and Genomic Analysis of a Novel Drexlervirial Bacteriophage IME268 with Lytic Activity Against Klebsiella pneumoniae

Genomic Analysis of Bacteriophage BUCT86 Infecting Klebsiella Pneumoniae

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