Biochemical and genetic characterization of Arabidopsis flavanone 3β-hydroxylase

Owens, Daniel K.; Crosby, Kevin C.; Runac, Justin; Howard, Brad; Winkel, Brenda S.J.

doi:10.1016/j.plaphy.2008.06.004

Cited by 89 publications

(86 citation statements)

References 75 publications

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“…Furthermore, an in vitro enzyme assay showed that the cotton F3H enzyme had optimum activity at pH 8.5 and 30°C (Fig. 3, A and B), which was similar to that of its homolog in Arabidopsis (Owens et al, 2008). Previous results have indicated that F3H is highly expressed in fiber cells (Supplemental Table S1).…”

Section: Characterization Of F3h In Cottonsupporting

confidence: 58%

“…The F3H mutant or down-regulated plants exhibit petal color disruption and flavonoid content reduction (Britsch, 1990;Stephens et al, 1993;Peer et al, 2001;Flachowsky et al, 2012). Additionally, the development of seeds and seedlings is also affected in Arabidopsis and soybean (Glycine max) F3H mutants (Debeaujon et al, 2000;Zabala and Vodkin, 2005;Owens et al, 2008;Buer and Djordjevic, 2009). Previous work also showed that the transcript of F3H was abundant in cotton (Gossypium spp.…”

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A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin

et al. 2013

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The cotton (Gossypium spp.) fiber is a unique elongated cell that is useful for investigating cell differentiation. Previous studies have demonstrated the importance of factors such as sugar metabolism, the cytoskeleton, and hormones, which are commonly known to be involved in plant cell development, while the secondary metabolites have been less regarded. By mining public data and comparing analyses of fiber from two cotton species (Gossypium hirsutum and Gossypium barbadense), we found that the flavonoid metabolism is active in early fiber cell development. Different flavonoids exhibited distinct effects on fiber development during ovule culture; among them, naringenin (NAR) could significantly retard fiber development. NAR is a substrate of flavanone 3-hydroxylase (F3H), and silencing the F3H gene significantly increased the NAR content of fiber cells. Fiber development was suppressed following F3H silencing, but the overexpression of F3H caused no obvious effects. Significant retardation of fiber growth was observed after the introduction of the F3H-RNA interference segment into the high-flavonoid brown fiber G. hirsutum T586 line by cross. A greater accumulation of NAR as well as much shorter fibers were also observed in the BC1 generation plants. These results suggest that NAR is negatively associated with fiber development and that the metabolism mediated by F3H is important in fiber development, thus highlighting that flavonoid metabolism represents a novel pathway with the potential for cotton fiber improvement.

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Section: Characterization Of F3h In Cottonsupporting

confidence: 58%

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confidence: 99%

A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin

et al. 2013

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“…Besides this, it is also possible that another related two oxoglutarate dioxygenase can complement the lack of FLS1 (and LDOX). One candidate is F3H, which is thought to have overlapping in vivo activities with FLS1 and LDOX (Owens et al 2008b). These proposals need further investigation.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase

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et al. 2008

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Flavonol synthase (FLS) (EC-number 1.14.11.23), the enzyme that catalyses the conversion of Xavonols into dihydroXavonols, is part of the Xavonoid biosynthesis pathway. In Arabidopsis thaliana, this activity is thought to be encoded by several loci. In addition to the FLAVONOL SYNTHASE1 (FLS1) locus that has been conWrmed by enzyme activity assays, loci displaying similarity of the deduced amino acid sequences to FLS1 have been identiWed. We studied the putative A. thaliana FLS gene family using a combination of genetic and metabolite analysis approaches. Although several of the FLS gene family members are expressed, only FLS1 appeared to inXuence Xavonoid biosynthesis. Seedlings of an A. thaliana Xs1 null mutant (Xs1-2) show enhanced anthocyanin levels, drastic reduction in Xavonol glycoside content and concomitant accumulation of glycosylated forms of dihydroXavonols, the substrate of the FLS reaction. By using a leucoanthocyanidin dioxygenase (ldox) Xs1-2 double mutant, we present evidence that the remaining Xavonol glycosides found in the Xs1-2 mutant are synthesized in planta by the FLS-like side activity of the LDOX enzyme.

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“…Our results suggest that the selective degradation of BBX22 might fine-tune the expression of ELIP2 for optimal chlorophyll accumulation. BBX22 also up-regulates genes involved in flavonoid and anthocyanin biosynthesis pathways, including CHS, CHI, F3H, and 4CL3 (Li et al, 1993;Shirley et al, 1995;Ehlting et al, 1999;Raes et al, 2003;Solfanelli et al, 2006;Poustka et al, 2007;Owens et al, 2008;Buer and Djordjevic, 2009). This observation explains the excess accumulation of anthocyanin in etiolated cop1BBX22-GFPox plants (Fig.…”

Section: Bbx22 Influences Genes On Light Signaling and Hormone Responsesmentioning

confidence: 99%

COP1-Mediated Degradation of BBX22/LZF1 Optimizes Seedling Development in Arabidopsis

2011

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Light regulates multiple aspects of growth and development in plants. Transcriptomic changes govern the expression of signaling molecules with the perception of light. Also, the 26S proteasome regulates the accumulation of positive and negative regulators for optimal growth of Arabidopsis (Arabidopsis thaliana) in the dark, light, or light/dark cycles. BBX22, whose induction is both light regulated and HY5 dependent, is a positive regulator of deetiolation in Arabidopsis. We found that during skotomorphogenesis, the expression of BBX22 needs to be tightly regulated at both transcriptional and posttranslational levels. During photomorphogenesis, the expression of BBX22 transiently accumulates to execute its roles as a positive regulator. BBX22 protein accumulates to a higher level under short-day conditions and functions to inhibit hypocotyl elongation. The proteasome-dependent degradation of BBX22 protein is tightly controlled even in plants overexpressing BBX22. An analysis of BBX22 degradation kinetics shows that the protein has a short half-life under both dark and light conditions. COP1 mediates the degradation of BBX22 in the dark. Although dispensable in the dark, HY5 contributes to the degradation of BBX22 in the light. The constitutive photomorphogenic development of the cop1 mutant is enhanced in cop1BBX22ox plants, which show a short hypocotyl, high anthocyanin accumulation, and expression of light-responsive genes. Exaggerated light responsiveness is also observed in cop1BBX22ox seedlings grown under short-day conditions. Therefore, the proper accumulation of BBX22 is crucial for plants to maintain optimal growth when grown in the dark as well as to respond to seasonal changes in daylength.

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Biochemical and genetic characterization of Arabidopsis flavanone 3β-hydroxylase

Cited by 89 publications

References 75 publications

A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin

A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin

Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase

COP1-Mediated Degradation of BBX22/LZF1 Optimizes Seedling Development in Arabidopsis

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