Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase

Stracke, Ralf; Vos, Ric C. H. de; Bartelniewoehner, Lutz; Ishihara, Hirofumi; Sagasser, Martin; Martens, Stefan; Weißhaar, Bernd

doi:10.1007/s00425-008-0841-y

Cited by 118 publications

(151 citation statements)

References 64 publications

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“…5C). This is in line with previous results, suggesting an alternative route to produce flavonols in plants by takeover of DFR activity (Wilmouth et al, 2002;Wellmann et al, 2006;Stracke et al, 2009).…”

Section: Vvmybf1 Specifically Activates Select Flavonol Pathway Genessupporting

confidence: 93%

The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries

et al. 2009

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Flavonols are important ultraviolet light protectants in many plants and contribute substantially to the quality and healthpromoting effects of fruits and derived plant products. To study the regulation of flavonol synthesis in fruit, we isolated and characterized the grapevine (Vitis vinifera 'Shiraz') R2R3-MYB transcription factor VvMYBF1. Transient reporter assays established VvMYBF1 to be a specific activator of flavonol synthase1 (VvFLS1) and several other promoters of grapevine and Arabidopsis (Arabidopsis thaliana) genes involved in flavonol synthesis. Expression of VvMYBF1 in the Arabidopsis mutant myb12 resulted in complementation of its flavonol-deficient phenotype and confirmed the function of VvMYBF1 as a transcriptional regulator of flavonol synthesis. Transcript analysis of VvMYBF1 throughout grape berry development revealed its expression during flowering and in skins of ripening berries, which correlates with the accumulation of flavonols and expression of VvFLS1. In addition to its developmental regulation, VvMYBF1 expression was light inducible, implicating VvMYBF1 in the control of VvFLS1 transcription. Sequence analysis of VvMYBF1 and VvFLS1 indicated conserved putative light regulatory units in promoters of both genes from different cultivars. By analysis of the VvMYBF1 amino acid sequence, we identified the previously described SG7 domain and an additional sequence motif conserved in several plant MYB factors. The described motifs have been used to identify MYB transcription factors from other plant species putatively involved in the regulation of flavonol biosynthesis. To our knowledge, this is the first functional characterization of a light-inducible MYB transcription factor controlling flavonol synthesis in fruit.

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Section: Vvmybf1 Specifically Activates Select Flavonol Pathway Genessupporting

confidence: 93%

The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries

et al. 2009

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“…Similar staining experiments performed in Arabidopsis identified yellow and green DPBA-mediated fluorescent compounds as quercetin and kaempferol (flavonols) and blue fluorescence-emitting compounds as sinapate glycoside derivatives (Stracke et al, 2007(Stracke et al, , 2009). Application of additional staining, including Shinoda's test and Zn-HCl (Pew's test) on yellow and white F 3 methanol extracts (Supplemental Fig.…”

Section: Characterization Of Biochemical Differences Between Two Muskmentioning

confidence: 54%

A Kelch domain-containing F-box coding gene negatively regulates flavonoid accumulation in Cucumis melo L.

Feder

Burger²,

Gao

et al. 2015

Plant Physiol.

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The flavonoids are phenylpropanoid-derived metabolites that are ubiquitous in plants, playing many roles in growth and development. Recently, we observed that fruit rinds of yellow casaba muskmelons (Cucumis melo 'Inodorous Group') accumulate naringenin chalcone, a yellow flavonoid pigment. With RNA-sequencing analysis of bulked segregants representing the tails of a population segregating for naringenin chalcone accumulation followed by fine mapping and genetic transformation, we identified a Kelch domain-containing F-box protein coding (CmKFB) gene that, when expressed, negatively regulates naringenin chalcone accumulation. Additional metabolite analysis indicated that downstream flavonoids are accumulated together with naringenin chalcone, whereas CmKFB expression diverts the biochemical flux toward coumarins and general phenylpropanoids. These results show that CmKFB functions as a posttranscriptional regulator that diverts flavonoid metabolic flux.

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“…Since no FLS like sequence was obtained during our cloning studies and the expression of related Xavonol/anthocyanin pathway genes such as FHT, DFR and ANS is rather low and speciWc compared to the transcripts of Xavone biosynthesis, the absence of Xavonols is likely. However, due to the described site activity of ANS (Stracke et al 2009) the synthesis of Xavonols cannot be excluded and they might escape detection due to low, tissue speciWc, or spatial occurrence. UGT90A7 showed highest activity with eriodictyol and luteolin.…”

Section: Discussionmentioning

confidence: 99%

Recombinant expression and functional characterisation of regiospecific flavonoid glucosyltransferases from Hieracium pilosella L.

Witte

Moco

Vervoort

et al. 2009

Planta

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Five glucosyltransferases were cloned by RT-PCR ampliWcation using total RNA from Hieracium pilosella L. (Asteraceae) inXorescences as template. Expression was accomplished in Escherichia coli, and three of the HIS-tagged enzymes, UGT90A7, UGT95A1, and UGT72B11 were partially puriWed and functionally characterised as UDP-glucose:Xavonoid O-glucosyltransferases. Both UGT90A7 and UGT95A1 preferred luteolin as substrate, but possessed diVerent regiospeciWcity proWles. UGT95A1 established a new subgroup within the UGT family showing high regiospeciWcity towards the C-3Ј hydroxyl group of luteolin, while UGT90A7 primarily yielded the 4Ј-O-glucoside, but concomitantly catalysed also the formation of the 7-O-glucoside, which could account for this Xavones glucoside in H. pilosella Xower heads. Semi quantitative expression proWles revealed that UGT95A1 was expressed at all stages of inXorescence development as well as in leaf and stem tissue, whereas UGT90A7 transcript abundance was nearly limited to Xower tissue and started to develop with the pigmentation of closed buds. Other than these enzymes, UGT72B11 showed rather broad substrate acceptance, with highest activity towards Xavones and Xavonols which have not been reported from H. pilosella. As umbelliferone was also readily accepted, this enzyme could be involved in the glucosylation of coumarins and other metabolites.

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Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase

Cited by 118 publications

References 64 publications

The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries

The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries

A Kelch domain-containing F-box coding gene negatively regulates flavonoid accumulation in Cucumis melo L.

Recombinant expression and functional characterisation of regiospecific flavonoid glucosyltransferases from Hieracium pilosella L.

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