2005
DOI: 10.1016/j.bbagen.2005.05.022
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Biochemical and enzymatic characterization of two basic Asp49 phospholipase A2 isoforms from Lachesis muta muta (Surucucu) venom

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Cited by 41 publications
(49 citation statements)
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“…1, Table 1) display high N-terminal sequence similarity to the hemolytic and platelet aggregation inhibitory Lm-PLA 2 -I and Lm-PLA 2 -II characterized by Fully et al [47] from L. muta venom provided by Sigma or Fundação Ezequiel Dias (FUNED, Brazil), to LMPA1 (P84651) from the same source, and to two basic neurotoxic Asp49 PLA 2 molecules (LmTX-I and LmTX-II) isolated from L. muta venom purchased from Sigma [48,49] (Table 4). In particular, the N-terminal sequence of the PLA 2 isolated in fraction Lm18 (Table 1) seems to be identical to that of LM-PLA2-II, except for the striking lack of the serine residue at position 16.…”
Section: Moleculesmentioning
confidence: 99%
“…1, Table 1) display high N-terminal sequence similarity to the hemolytic and platelet aggregation inhibitory Lm-PLA 2 -I and Lm-PLA 2 -II characterized by Fully et al [47] from L. muta venom provided by Sigma or Fundação Ezequiel Dias (FUNED, Brazil), to LMPA1 (P84651) from the same source, and to two basic neurotoxic Asp49 PLA 2 molecules (LmTX-I and LmTX-II) isolated from L. muta venom purchased from Sigma [48,49] (Table 4). In particular, the N-terminal sequence of the PLA 2 isolated in fraction Lm18 (Table 1) seems to be identical to that of LM-PLA2-II, except for the striking lack of the serine residue at position 16.…”
Section: Moleculesmentioning
confidence: 99%
“…La enzima es de carácter ácido al ser eluída en el volumen isocrático del primer paso cromatográfico. En trabajos previos realizados en veneno de L. muta de Brasil han caracterizado cuatro isoformas de PLA 2 , siendo dos ácidas y dos básicas (Fuly et al 2002, Damico et al 2005, con características bioquímicas y biológicas distintas a las encontradas en la enzima purificada en este trabajo.…”
Section: Purificación De La Enzima Y Determinación Del Peso Molecularunclassified
“…Estudos detalhados da estrutura primária de peptídeos e proteí-nas com atividade farmacológica proveniente da amostra de veneno de diversas espécies animais do Brasil, incluindo Tityus serrulatus, Lonomia obliqua e Bothrops insularis, foram desenvolvidos com o auxílio da espectrometria de massas Q-TOF ("Quadrupole-Time of Flight": quadrupolo-tempo de vôo) 16,17 . A identificação da estrutura primária das proteínas com atividade farmacológica faz parte de um trabalho mais amplo que tem como objetivo final o desenvolvimento de novos fármacos.…”
Section: Proteômicaunclassified