Biochemical analyses of Dendrobium Sabin Blue PLBs during cryopreservation by vitrification

Antony, Jessica Jeyanthi James; Zakaria, Shahsuzan; Zakaria, Rahmad; Ujang, Jorim Anak; Othman, Nurulhasanah; Subramaniam, Sreeramanan

doi:10.1007/s12298-019-00703-2

Cited by 11 publications

(7 citation statements)

References 58 publications

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“…This signi ed the stressful dehydration conditions resulted in an overproduction of proline in cryopreserved axillary buds. Similarly, cryopreserved Dendrobium Sabin Blue exhibited the highest proline level after dehydration with PVS2 treatment (Antony et al 2019). According to several studies, amino acid such as proline occurs typically in the cytoplasm and contributes to stress tolerance by behaving as a molecular chaperon that maintains the membrane integrity, alters cell osmotic pressure, and remove ROS (Hayat et al 2012;Soshinkova et al 2013;Dar et al 2016).…”

Section: Biochemical Analyses During Cryopreservationmentioning

confidence: 96%

“…The POX assay was evaluated following the protocol based on Sánchez-Rojo et al ( 2015) and Antony et al (2019). The protein extract was obtained by grinding 100 mg of axillary buds in an iced cold mortar and homogenized with an enzyme extraction buffer solution.…”

Section: Peroxidase (Pox) Assaymentioning

confidence: 99%

“…On the other hand, cryopreserved axillary buds enhanced stress tolerance by increasing the synthesis of enzymatic antioxidants: CAT and APX (initial stages of cryopreservation) and POX (immediately after LN exposure followed with rewarming) that breaks down and remove the excess free radicals. The increased expression of antioxidants among the critical stages of cryopreservation may be due to the high production of H 2 O 2 , which in return triggers antioxidant activities as ROS scavengers (Antony et al 2019;Vianna et al 2019). However, the reduced expressions of antioxidant enzyme activities re ected the cryopreserved explants' inability to combat the accumulation of ROS, which in return led to the poor survival of explants and was associated with tissue browning reported by Rahmah et al (2015).…”

Section: Biochemical Analyses During Cryopreservationmentioning

confidence: 99%

“…The stresses that occurred included excision of plant materials, osmotic injury and dehydration following treatment with highly concentrated cryoprotectants, and freezing injury from sudden temperature changes. Consequently, oxidative damage occurs due to the increased accumulation of reactive oxygen species (ROS) (Uchendu et al 2010;Antony et al 2019). ROS including superoxide radical (O 2 ¯), hydrogen peroxide (H 2 O 2 ), hydroxyl radical (OH ¯), and singlet oxygen ( 1 O 2 ) is produced in chloroplasts, mitochondria, and peroxisomes (Suzuki et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Cryopreservation Method Supported With Biochemical Analyses in the Axillary Bud of Jewel Orchid, Ludisia Discolor

Burkhan

Rajan

Appalasamy

et al. 2021

Preprint

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This study was conducted to investigate the potential of conserving an endangered terrestrial jewel orchid Ludisia discolor using in vitro grown axillary buds. Excised segments of axillary buds (4 - 5 mm in length) were precultured on a modified Murashige and Skoog (MS) medium supplemented with 0.2 M sucrose for 24 h and osmoprotected in a loading solution for 20 min. Then, axillary buds were dehydrated in PVS2 solution for 10 min at 0°C and incubated in liquid nitrogen for 1 h. Subsequently, axillary buds were rewarmed rapidly by dilution solution and transferred to a growth recovery medium supplemented with 0.05 µM melatonin under in vitro conditions that led to an improved survival chance (16.67%) for cryopreserved L. discolor. The abiotic stresses and the overproduction of reactive oxygen species (ROS) during cryopreservation stages may contribute to cryoinjuries and poor survival. The varied response towards stress was detected with significantly increased values recorded at certain cryopreservation stages, including proline activity at the dehydration stage (5.51 µmol/g), catalase at the preculture (85.64 U/g) and dehydration (70.87 U/g) stages, peroxidase at the rewarming stage (565.37 U/g) and ascorbate peroxidase during the loading stage (12.19 U/g). Hence, this first attempt to cryopreserved L. discolor indicates that future experimental designs could include exogenous antioxidants and different vitrification solutions to improve survival and regeneration.

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Section: Biochemical Analyses During Cryopreservationmentioning

confidence: 96%

Section: Peroxidase (Pox) Assaymentioning

confidence: 99%

Section: Biochemical Analyses During Cryopreservationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Cryopreservation Method Supported With Biochemical Analyses in the Axillary Bud of Jewel Orchid, Ludisia Discolor

Burkhan

Rajan

Appalasamy

et al. 2021

Preprint

Self Cite

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“…PLBs of D. officinale have the activities of treating skin problems and strengthening the immune system, and they have antioxidant, anti-tumor, anti-cancer, and anti-inflammatory activities [ 7 , 8 , 9 ]. At present, the research of PLBs of D. officinale has mainly focused on cultivation [ 10 , 11 ], long-term preservation [ 12 ], gene expression [ 13 , 14 ], etc., and there are few reports on the active components in PLBs [ 15 , 16 ], among which the separation of glycosides from PLBs of D. officinale has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Combined Ultrahigh Pressure Extraction and High-Speed Counter-Current Chromatography for Separation and Purification of Three Glycoside Compounds from Dendrobium officinale Protocorm

Zhang

Wang

et al. 2021

Molecules

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As an alternative to Dendrobium candidum, protocorm-like bodies (PLBs) of Dendrobium candidum are of great value due to their high yield and low cost. In this work, three glycoside compounds, β-D-glucopyranose 1-[(E)-3-(4-hydroxyphenyl)-2-propenoat] (I), β-D-glucopyranose 1-[(E)-3-(3, 4-dihydroxyphenyl)-2-propenoat] (II), and 1-O-sinapoyl glucopyranoside (III), were extracted and isolated by ultrahigh pressure extraction (UPE) coupled with high-speed counter-current chromatography (HSCCC) from PLBs of D. officinale. First, the target compounds were optimized and prepared with 50% ethanol solution at a 1:30 (g/mL) solid/liquid ratio in 2 min under 300 MPa by UPE. Then, the crude extract was chromatographed with a silica gel column, and primary separation products were obtained. In addition, the products (150 mg) were separated by HSCCC under the solvent system of MTBE-n-butyl alcohol-acetonitrile-water (5:1:2:6, v/v/v/v), yielding 31.43 mg of compound I, 10.21 mg of compound II, and 24.75 mg of compound III. Their structures were further identified by ESI-MS, 1H NMR, and 13C NMR. The antioxidant results showed that the three compounds expressed moderate effects on the DPPH· scavenging effect. Compound II had the best antioxidant capacity and its IC50 value was 0.0497 mg/mL.

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ROS-induced oxidative stress in plant cryopreservation: occurrence and alleviation

Ren

Wang

2021

Planta

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Main conclusion Reactive oxygen species (ROS)-induced oxidative stress results in low success or even total failure of cryopreservation. Better understanding of how the plant establishes resistance/tolerance to ROS-induced oxidative stress facilitates developments of robust cryopreservation procedures. Abstract Cryopreservation provides a safe and efficient strategy for long-term preservation of plant genetic resources. ROS-induced oxidative stress caused damage to cells and reduced the ability of the plant to survive following cryopreservation, eventually resulting in low success or even total failure. This paper provides updated and comprehensive information obtained in the past decade, including the following: (1) ROS generations and adaptive responses of antioxidant systems during cryopreservation; (2) expressions of oxidative stress-associated genes and proteins during cryopreservation; (3) ROS-triggered programmed cell death (PCD) during cryopreservation; and (4) exogenous applications of enzymatic and non-enzymatic antioxidants in improving success of cryopreservation. Prospects for further studies are proposed. The goal of the present study was to facilitate better understanding of the mechanisms by which the plant establishes resistance/tolerance to oxidative stress during cryopreservation and promote further studies toward the developments of robust cryopreservation procedures and wider application of plant cryobiotechnology.

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Biochemical analyses of Dendrobium Sabin Blue PLBs during cryopreservation by vitrification

Cited by 11 publications

References 58 publications

Effect of Cryopreservation Method Supported With Biochemical Analyses in the Axillary Bud of Jewel Orchid, Ludisia Discolor

Effect of Cryopreservation Method Supported With Biochemical Analyses in the Axillary Bud of Jewel Orchid, Ludisia Discolor

Combined Ultrahigh Pressure Extraction and High-Speed Counter-Current Chromatography for Separation and Purification of Three Glycoside Compounds from Dendrobium officinale Protocorm

ROS-induced oxidative stress in plant cryopreservation: occurrence and alleviation

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