Biochar addition reinforces microbial interspecies cooperation in methanation of sugar beet waste (pulp)

Pytlak, Anna; Kasprzycka, Agnieszka; Szafranek-Nakonieczna, Anna; Grządziel, Jarosław; Kubaczyński, Adam; Proc, Kinga; Onopiuk, Paulina; Walkiewicz, Anna; Polakowski, Cezary; Gałązka, Anna; Lalak-Kańczugowska, Justyna; Stępniewska, Zofia; Bieganowski, Andrzej

doi:10.1016/j.scitotenv.2020.138921

Cited by 27 publications

(8 citation statements)

References 42 publications

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“…Ruminofilibacter and Bacillus also had a relative high abundance. Solibacillus can secrete different enzymes for substrate degradation, and Clostridium was an important microorganism in degrading proteins and polysaccharides in previous studies [41,42]. Therefore, they were widely present in CK and TG.…”

Section: Bacterial Community Characteristicsmentioning

confidence: 99%

Enhanced Biogas Production by Ligninolytic Strain Enterobacter hormaechei KA3 for Anaerobic Digestion of Corn Straw

Zhang

Zhao

et al. 2021

Energies

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Lignin-feeding insect gut is a natural ligninolytic microbial bank for the sustainable conversion of crop straw to biogas. However, limited studies have been done on highly efficient microbes. Here, an efficient ligninolytic strain Enterobacter hormaechei KA3 was isolated from the gut microbiomes of lignin-feeding Hypomeces squamosus Fabricius, and its effects on lignin degradation and anaerobic digestion were investigated. No research has been reported. Results showed that strain KA3 had better lignin-degrading ability for corn straw with a higher lignin-degrading rate (32.05%) and lignin peroxidase activity (585.2 U/L). Furthermore, the highest cumulative biogas yield (59.19 L/kg-VS) and methane yield (14.76 L/kg-VS) were obtained for KA3 inoculation, which increased by 20% and 31%, respectively, compared to CK. Higher removal rates of COD, TS, and vs. of 41.6%, 43.11%, and 66.59% were also found. Moreover, microbial community diversity increased as digestion time prolonged in TG, and bacteria were more diverse than archaea. The dominant genus taxon, for methanogens, was Methanosate in TG, while in CK was Methanosarcina. For bacteria, dominant taxa were similar for all groups, which were Solibacillus and Clostridium. Therefore, strain KA3 improved the methane conversion of the substrate. This study could provide a new microbial resource and practical application base for lignin degradation.

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Section: Bacterial Community Characteristicsmentioning

confidence: 99%

Enhanced Biogas Production by Ligninolytic Strain Enterobacter hormaechei KA3 for Anaerobic Digestion of Corn Straw

Zhang

Zhao

et al. 2021

Energies

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“…Biochar is known as a highly porous conductive material, and several studies reported that microorganisms, such as methanogenic archaea and hydrophobic microbiota, could colonize the biochar microporosities − as those shown with 1000 and 250 magnifications (Figure S9). SEM images of biochar samples demonstrated the presence of coccus and bacillus with 0.5 and 6.5 μm lengths on the outer and inner ( e.g.…”

Section: Resultsmentioning

confidence: 89%

Conversion of Pyrolysis Products into Volatile Fatty Acids with a Biochar-Packed Anaerobic Bioreactor

Küçükağa

Facchin

Kara

et al. 2022

Ind. Eng. Chem. Res.

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The coupling of pyrolysis and acidogenic fermentation was here proposed as a new hybrid thermochemical-biological method to circumvent the hydrolysis bottleneck within lignocellulose valorization schemes. Pyrolysis products of fir sawdust, that is, the water-soluble (WS) fraction together with CO-rich syngas, were tested as feedstock for volatile fatty acid (VFA) production. WS/syngas conversion to VFA was particularly challenging due to the combined effect of the substrate (WS/syngas) and product (VFA) inhibition. To solve such an issue, a new type of bioreactor, based on packed biochar and a new acclimatization/bioaugmentation procedure consisting of co-feeding WS/syngas and glucose were developed and tested. The gradual switch from glucose to WS was monitored through various analytical techniques, observing the transition toward a “pyrotrophic” microbial mixed culture able to convert WS/syngas into VFA. Even without selective inhibition of methanogens, the main fermentation products were VFA (mainly acetic, butyric, and caproic acid), whose profile was a function of the WS/glucose ratio. Although the achieved volumetric productivity was lower (<0.6 gCOD L–1 d–1) than that observed in sugar fermentation, bioaugmented pyrotrophs could convert headspace CO, most of GC–MS detectable compounds (e.g., anhydrosugars), and a significant portion of non-GC–MS detectable compounds of WS (e.g., oligomers with MW < 1.45 kDa).

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“…Pytlak et al (2020) observed an increase in CH4 content from 54 to 62% due to biochar addition at 1.25 g/L. Carbon based material addition also helped to reduce H2S concentration in biogas(Wang et al 2019c).…”

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confidence: 87%

Promotion of direct interspecies electron transfer and potential impact of conductive materials in anaerobic digestion and its downstream processing - a critical review

Nguyen

Johir

et al. 2021

Bioresource Technology

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Biochar addition reinforces microbial interspecies cooperation in methanation of sugar beet waste (pulp)

Cited by 27 publications

References 42 publications

Enhanced Biogas Production by Ligninolytic Strain Enterobacter hormaechei KA3 for Anaerobic Digestion of Corn Straw

Enhanced Biogas Production by Ligninolytic Strain Enterobacter hormaechei KA3 for Anaerobic Digestion of Corn Straw

Conversion of Pyrolysis Products into Volatile Fatty Acids with a Biochar-Packed Anaerobic Bioreactor

Promotion of direct interspecies electron transfer and potential impact of conductive materials in anaerobic digestion and its downstream processing - a critical review

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