Biocatalyzed Sulfoxidation in Presence of Deep Eutectic Solvents

Abstract

The flavin-containing monooxygenase from Methylophaga sp. strain SK1 (mFMO) is a valuable biocatalyst for the preparation of optically active sulfoxides, among other valuable compounds. In this study, we explored to benefits of using Natural Deep Eutectic Solvents (NADESs) when doing oxidation with this biocatalyst, fused to phosphite dehydrogenase for cofactor regeneration (PTDH-mFMO). It was found that optically active sulfoxides could be obtained with slightly higher conversions in 10% v/v NADES when working at substrate concentrations of 50–200 mM, whereas there was no loss in the enantioselectivity. With these results, it is demonstrated for the first time that flavin-containing monooxygenases can be employed as biocatalysts in presence of NADESs.

Can Deep Eutectic Solvents Sustain Oxygen-Dependent Bioprocesses?—Measurements of Oxygen Transfer Rates

“…To the best our knowledge, the transfer rates of dioxygen into DESs are not reported yet, and thus, comprehensive data are not available until now. Having highlighted that, oxygen-dependent enzymes such as laccases, 52 oxidases, 53 and monooxygenases 54 have been recently used either in DESs as predominant reaction media or in the presence of DESs as cosolvents. The study presented here reports on the first measurements of oxygen transfer from air into DESs and DES−water mixtures.…”

Impact of deep eutectic solvents (DESs) and individual DES components on alcohol dehydrogenase catalysis: connecting experimental data and molecular dynamics simulations

“…46 The enzymatic activity of ADHs could be maintained in DES-water mixtures (>10 vol% water) and outperformed pure DESs, illustrating the benefits of adding water. 40,47 The effects of individual DES components on enzyme catalysis, together with the determination of the optimal water concentration, have to be addressed for a practical application of DESs. Previously, we reported an analysis of the bioreduction catalyzed by horse-liver alcohol dehydrogenase (HLADH) in a choline chloride and glycerol DES (ChCl-Gly, molar ratio 1 : 2) using up to 20 vol% of water.…”