Impact of deep eutectic solvents (DESs) and individual DES components on alcohol dehydrogenase catalysis: connecting experimental data and molecular dynamics simulations

Abstract

For a knowledge-based design of enzyme catalysis in deep eutectic solvents (DESs), the influence of the DESs properties (e.g., water activity, viscosity), and the impact of DESs and their individual...

Deep eutectic solvents as a stabilising medium for NAD coenzyme: unravelling the mechanism behind coenzyme stabilisation effect

“…By shifting water content in DES toward higher values, the activity of the enzymes usually increases and at optimal conditions reaches higher values than those in reference systems. 67–70…”

“…In such DES the enzyme was highly active and stable, giving promising productivity. 68 To be more precise, a high HLADH half-life time peak was observed for ChCl:Gly at a water content of 40 vol% (higher than the half-life time observed in the aqueous buffer), which decreased at higher water contents. Even though we have shown that Ch:Gly at water content up to 35% is one of the least suitable DES for stabilisation of NAD coenzyme, these mixtures still showed improved coenzyme stabilisation ability compared to reference phosphate buffer commonly used for dehydrogenase-catalysed reactions, implying its potential as a storage medium.…”

“…By shifting water content in DES toward higher values, the activity of the enzymes usually increases and at optimal conditions reaches higher values than those in reference systems. [67][68][69][70] Even though the influence of DES on enzymes has been closely examined, the behaviour of oxidoreductive enzymes in DES, especially those that are NAD dependent, is still not systematically explored. Ma et al 71 studied ChCl-based DES with urea and polyols as alternative solvents for peroxygenase-catalysed reactions by using choline oxidase from A. nicotianae and the recombinant peroxygenase from A. aegerita as catalysts in the bienzymatic cascade for the selective oxyfunctionalisation of ethyl benzene or cis-β-methylstyrene.…”

Enzymatic Cascade for the Synthesis of 2,5‐Furandicarboxylic Acid in Biphasic and Microaqueous Conditions: ‘Media‐Agnostic’ Biocatalysts for Biorefineries

“…[7] Recent developments showed great potential of using enzymes in low-water media, which became not limited to lipases. [8] When it comes to biorefineries, the possibility that enzymes can efficiently catalyze reactions in media containing different water proportions is clearly an asset, as it provides adaptation to varied (crude) effluent types.…”

“…Non‐conventional media encompass a wide array of potential reaction systems, ranging from ionic liquids (ILs), deep eutectic solvents (DES), neat solvents, microaqueous reaction systems (MARS), and biphasic systems consisting of water and organic media [7] . Recent developments showed great potential of using enzymes in low‐water media, which became not limited to lipases [8] . When it comes to biorefineries, the possibility that enzymes can efficiently catalyze reactions in media containing different water proportions is clearly an asset, as it provides adaptation to varied (crude) effluent types.…”