Biocatalytic strategies in the production of galacto-oligosaccharides and its global status

Panesar, Parmjit S.; Kaur, Rupinder; Singh, Ram Sarup; Kennedy, John F.

doi:10.1016/j.ijbiomac.2018.01.062

Cited by 55 publications

(20 citation statements)

References 115 publications

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“…Therefore, it is evident that the most efficient immobilized preparations, in terms of all examined parameters were the primary amino functionalized carriers. Examples of amino functionalized resin usage for β‐galactosidase from A. oryzae are often present in the literature (Panesar et al ., ), even though the physical adsorption of the enzyme, that presumably occurs via electrostatic interactions between support amino groups and carboxylic groups in side chains of enzyme amino acids (in this case), represents unfavorable type of immobilization protocol in terms of the immobilized preparation stability (Carević et al ., ; Panesar et al ., ). However, a simple protocol, mild immobilization conditions, minimal damage of catalytic activity, and potentially easy carrier recovery coupled with high expressed activity seemed as good choice for future β‐galactosidase immobilization.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, with the genuine progress of biotechnology whey is becoming a substrate of choice for diverse food and pharmaceutical industries (Kareb & A€ ıder, 2018;Lata et al, 2018). With the increased interest in functional foods and prebiotics, one of the promising approaches for whey valorization through conversion of present lactose by virtue of transgalactosylation activity of b-galactosidase (Torres et al, 2010;Vera et al, 2016;Panesar et al, 2018). Namely, wide range of physiologically active compounds collectively named galacto-oligosaccharides (GOS) could be produced by b-galactosidase mediated transfer of galactosyl moiety onto other saccharide molecules, resulting in the formation of a complex mixture of carbohydrates notably varying in polymerization degree, type of linkages, and their properties (Gosling et al, 2010;Carevi c et al, 2016a;Chen & G€ anzle, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Whey valorization using transgalactosylation activity of immobilized β‐galactosidase

Simović

Milivojević

Ćorović

et al. 2019

Int J of Food Sci Tech

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Summary Whey represents a significant dairy industry by‐product that has recently received due attention based on the rich nutrient composition and significant transformation potential. Hereby, we investigated a possibility of whey lactose bioconversion into prebiotic compounds, galacto‐oligosaccharides (GOS) using β‐galactosidase transgalactosylation activity. The results showed that whey could be successfully used for GOS synthesis, since the highest GOS concentration (around 62 g L−1) was obtained batchwise using 40% (w/w) sweet whey powder solution under optimum conditions (50°C, pH 4.5). Nevertheless, an efficient immobilized preparation using methacrylic Lifetech ECR8409 immobilization carrier was developed, enabling additional process improvement and ensuring at least 4 reaction cycles with unchanged yields and 2.5‐ fold enhanced productivity in comparison to the soluble enzyme. Therefore, this study provides a valuable contribution to the efficient and economical valorization of whey, which can be further on utilized as functional food and feed constituent.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Whey valorization using transgalactosylation activity of immobilized β‐galactosidase

Simović

Milivojević

Ćorović

et al. 2019

Int J of Food Sci Tech

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“…1,9 The yield and the composition of the synthesized GOS are influenced by the source of the enzyme and several reaction parameters including lactose concentration, pH, temperature, and water activity. [19][20][21] The percentage of GOS in the mixtures may vary in a wide range between 15-55 % (w/w) depending on such conditions. [22][23][24][25] The health promoting properties of GOS depend on their chemical composition, structure and degree of polymerization, which are highly influenced by the origin of the β-galactosidase.…”

Section: Introductionmentioning

confidence: 99%

Selective Synthesis of Galactooligosaccharides Containing β(1→3) Linkages with β-Galactosidase from Bifidobacterium bifidum (Saphera)

Füreder

Rodríguez-Colinas

Cervantes

et al. 2020

J. Agric. Food Chem.

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The transglycosylation activity of a novel commercial β-galactosidase from Bifidobacterium bifidum (Saphera) was evaluated. The optimal conditions of operation of this enzyme, measured with o-nitrophenyl-β-D-galactopyranoside, were 40 °C and pH around 6.0. Although at low lactose concentrations the character of this enzyme was basically hydrolytic, an increase of lactose concentration to 400 g/L resulted in a significant formation (107.2 g/L, 27% yield) of prebiotic galactooligosaccharides (GOS).The maximum amount of GOS was obtained at a lactose conversion of approximately 90%, which contrasts with other β-galactosidases, for which the highest GOS yield is achieved at 40-50% lactose conversion. Using HPAEC-PAD, semipreparative HPLC-HILIC, MS, 1D and 2D NMR, we determined the structure of most of the GOS synthesized by this enzyme.The main identified products were Gal-β(13)-Gal-β(14)-Glc (3´-O-Beta-galactosyllactose), Gal-β(1→6)-Glc (allolactose), Gal-β(13)-Glc (3-galactosyl-glucose), Galβ(1→3)-Gal (3-galactobiose) and the tetrasaccharide Gal-β(13)-Gal-β(13)-Galβ(14)-Glc. In general, the B. bifidum β-galactosidase showed a tendency to form β(13) linkages followed by β(16), and more scarcely β(14).

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“…At industrial scale, purification is done by simulated moving bed (SMB) chromatography, an expensive process regarding both capital expenditures and operating costs. For this reason, there is a continuous interest in developing cost‐effective separation methods for removing unwanted monomers and/or dimers from the crude GOS products (Panesar, Kaur, Singh, & Kennedy, 2018; Wisniewski, Pereira, Polakovic, & Rodrigues, 2014).…”

Section: Introductionmentioning

confidence: 99%

Production of high‐purity galacto‐oligosaccharides by depleting glucose and lactose from galacto‐oligosaccharide syrup with yeasts

Pázmándi

Kovács

Balga

et al. 2020

Yeast

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Galacto-oligosaccharides (GOS) are prebiotic compounds, widely used as ingredients in various food, nutraceutical and pharmaceutical products. Enzymatic synthesis of GOS results in low-purity products that contain high amounts of glucose and lactose beside the valuable GOS. In this study, a systematic approach was used to develop yeast-based fermentation strategies to purify crude GOS. Potentially applicable yeast strains were identified based on an extensive search in literature databases followed by a series of laboratory-scale fermentation tests. Single-and two-step fermentation processes were designed for the removal of glucose alone or together with lactose from crude GOS syrup. Single-step fermentation trials with two strains of previously unreported species, Cyberlindnera jadinii NCAIM Y.00499 and Kluyveromyces nonfermentans NCAIM Y.01443, resulted in purified products free of both glucose and ethanol from a crude GOS syrup diluted to 15 and 10 w/v%, respectively. Simultaneous removal of glucose and lactose was achieved by Kluyveromyces marxianus DMB Km-RK in a single-step fermentation process with a yield of 97.5% and final purity of 100%. A two-step fermentation approach was designed to allow conversion of a glucose-free product into a high-purity GOS by removing glucose with C. jadinii Y.00499 in the first step, and lactose by Kluyveromyces lactis DMB Kl-RK in the second step, resulting in a final product with a yield of 100% and a final purity of 92.1%. These results indicate that the selected nonconventional yeasts are promising candidates for the removal of non-GOS components from commercial crude GOS products by selective fermentation.

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Biocatalytic strategies in the production of galacto-oligosaccharides and its global status

Cited by 55 publications

References 115 publications

Whey valorization using transgalactosylation activity of immobilized β‐galactosidase

Whey valorization using transgalactosylation activity of immobilized β‐galactosidase

Selective Synthesis of Galactooligosaccharides Containing β(1→3) Linkages with β-Galactosidase from Bifidobacterium bifidum (Saphera)

Production of high‐purity galacto‐oligosaccharides by depleting glucose and lactose from galacto‐oligosaccharide syrup with yeasts

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