Biocatalytic Process Optimization

Kuo, Chia‐Hung; Shieh, Chwen‐Jen

doi:10.3390/catal10111303

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…In the recent years, the use of enzymes has become more popular in chemical and food processing [27], being the efficiency of catalytic activity dependent on reaction parameters such as pH, solvent and temperature, as well as the type of enzyme studied. Enzymes can be used in free or immobilized form for biocatalytic process [28]. Unfortunately, immobilized enzymes still suffer from higher prices compared to the free form, despite the stability and activity improvements that can be achieved by immobilization techniques [29].…”

Section: Co-immobilization Of Enzymes 21 Co-immobilization Of Enzymes Into Solid Supportsmentioning

confidence: 99%

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

et al. 2021

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During recent decades, the use of enzymes or chemoenzymatic cascades for organic chemistry has gained much importance in fundamental and industrial research. Moreover, several enzymatic and chemoenzymatic reactions have also served in green and sustainable manufacturing processes especially in fine chemicals, pharmaceutical, and flavor/fragrance industries. Unfortunately, only a few processes have been applied at industrial scale because of the low stabilities of enzymes along with the problematic processes of their recovery and reuse. Immobilization and co-immobilization offer an ideal solution to these problems. This review gives an overview of all the pathways for enzyme immobilization and their use in integrated enzymatic and chemoenzymatic processes in cascade or in a one-pot concomitant execution. We place emphasis on the factors that must be considered to understand the process of immobilization. A better understanding of this fundamental process is an essential tool not only in the choice of the best route of immobilization but also in the understanding of their catalytic activity.

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Section: Co-immobilization Of Enzymes 21 Co-immobilization Of Enzymes Into Solid Supportsmentioning

confidence: 99%

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

et al. 2021

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“…Enzymatic catalysis is carried out under moderated reaction conditions (pH, temperature, and atmospheric pressure), and the substrate is more regulated, allowing the production of high-quality natural products [ 11 , 12 ]. The advantages of biocatalysis are that they are suitable for industrial use, i.e., in their high specificity, selectivity, low energy consumption, and high yield [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrasound Plus Vacuum-System-Assisted Biocatalytic Synthesis of Octyl Cinnamate and Response Surface Methodology Optimization

Tsai

Huang

et al. 2022

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Cinnamic acid is one of the phenolic compounds that is isolated from cinnamon, or other natural plants, and has a wide range of physiological activities. However, the application of cinnamic acid is limited due to its poor solubility and low oral bioavailability. In this study, the feasibility of producing octyl cinnamate by ultrasonic assistance, combined with a rotary evaporation under vacuum, was studied using methyl cinnamate and octanol as the starting materials. A Box–Behnken design (BBD) was employed to evaluate the effects of the operation parameters, including reaction temperature (55–75 °C), reaction time (4–12 h), and ultrasonic power (90–150 W) on the production of octyl cinnamate. Meanwhile, the synthesis process was further optimized by the modeling response surface methodology (RSM). The data indicated that octyl cinnamate was efficiently synthesized from methyl cinnamate and octanol using the ultrasound plus vacuum system; further, this system was superior to the conventional method. According to the RSM model for the actual experiments, a reaction temperature of 74.6 °C, a reaction time of 11.1 h, and an ultrasound power of 150 W were determined to be the best conditions for the maximum molar conversion of octyl cinnamate (93.8%). In conclusion, the highly efficient synthesis of octyl cinnamate by a rotary evaporator with an ultrasound plus vacuum system was achieved via RSM optimization.

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Recent Advances in Overexpression of Functional Recombinant Lipases

et al. 2023

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Biocatalytic Process Optimization

Abstract: Biocatalysis refers to the use of microorganisms and enzymes in chemical reactions, has become increasingly popular and is frequently used in industrial applications due to the high efficiency and selectivity of biocatalysts [...]

Cited by 5 publications

References 27 publications

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

Ultrasound Plus Vacuum-System-Assisted Biocatalytic Synthesis of Octyl Cinnamate and Response Surface Methodology Optimization

Recent Advances in Overexpression of Functional Recombinant Lipases

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