Biocatalytic Growth of Au Nanoparticles:  From Mechanistic Aspects to Biosensors Design

Abstract

The H(2)O(2)-mediated enlargement of Au nanoparticles (NPs) and the growth mechanism are described. In addition to the deposition of gold on the NP faces, the formation of nanocrystalline clusters at the intersection of the faces is observed. The detachment of the latter nanoclusters provides additional seeds for the deposition of gold. The biocatalyzed generation of H(2)O(2) in the presence of O(2)/glucose and glucose oxidase enabled the development of an optical biosensor for glucose.

Nanomotor-based biocatalytic patterning of helical metal microstructures

“…As expected, no such microstructure was observed in the absence of the enzyme (Fig. 1B) 16 The formation of the helical Au patterning was also conrmed using the energy-dispersive X-ray (EDX) elemental mapping measurements shown in Fig. 2.…”

“…However, patterning of three-dimensional structures with high topological complexity of a helix represents a major fabrication challenge even with advanced lithographic techniques such as DPN. [16][17][18][19] Recent advances in nanomotors have facilitated the realization of the new nanomotor-based direct biocatalytic patterning method.These include the ability to navigate the motors along predetermined complex paths, to control and regulate their speed, to functionalize them with different biological or chemical entities, and to move them rapidly over large areas. 20,21 Previous studies reported that HRP-functionalized catalytic nanowire motors can be used for writing localized polymeric lines.…”

“…However, patterning of three-dimensional structures with high topological complexity of a helix represents a major fabrication challenge even with advanced lithographic techniques such as DPN. [16][17][18][19] Recent advances in nanomotors have facilitated the realization of the new nanomotor-based direct biocatalytic patterning method.…”

Highly sensitive on-site detection of glucose in human urine with naked eye based on enzymatic-like reaction mediated etching of gold nanorods

“…2D). The detection limit was calculated to be 0.1 μM (3σ rule), which is two orders of magnitude lower than other GNRs-based glucose sensors (Liu et al, 2013;Saa et al, 2014) and is almost the lowest among nanoparticle-based glucose sensors (Table S1, Supplementary Information) (Bi et al, 2015;Bo et al, 2013;Jiang et al, 2010;Kong et al, 2014;Luo et al, 2015;Radhakumary and Sreenivasan, 2011;Shen and Xia, 2014;Shiang et al, 2009;Su et al, 2012Su et al, , 2015bXia et al, 2013;Yi et al, 2013;Zayats et al, 2005;Zhao et al, 2015;Zheng et al, 2015). In addition, the small error bar of the data indicates the method has very high stability (Fig.…”

Microfluidic Paper‐Based Analytical Device for Sensitive Detection of Peptides Based on Specific Recognition of Aptamer and Amplification Strategy of Hybridization Chain Reaction

“…With the growth reaction went on wheels, the AuNP seeds were quickly grown after dropping the growth solution onto the working zone, which was driven by the selfcatalytic reduction mechanism of AuNP growth. [19] After 10 min of growth, a thick rod-like Au NPs conductive layer was prepared successfully. And each single fiber of the paper in the working zone owns full covering ofrod-like Au NPs ( Figure 1B).…”