Biocatalyst electrodes and their use in electrochemical syntheses.

Senda, Mitsugi; Ikeda, Tokuji; Hiasa, Hiroshi; Katasho, Isao

doi:10.1246/nikkashi.1987.358

Cited by 8 publications

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“…The Is value measured at -0.2 V increased linearly with increasing cI,, up to 0.5 mM, at which concentration a downward deviation was appreciable, as in the case of Figure 6. In the NOD electrode with a large amount of NOD, the effect of the diffusional process of the substrate in the enzyme layer and the membrane layer is expected to be significant, which leads to an extended linear range [15][16][17]. The response time of the electrode was 30 s, which was somewhat longer than that of the NOD electrode used in the previous section ( Figure 4).…”

Section: A Film-nod-cp(q)e As An Amperometricmentioning

confidence: 84%

“…Thus, theories of bioelectrocatalysis [15][16][17] and polarographic catalytic current [18][19][20] can be used to elucidate the experimental data, which predict a saturation tendency of the catalytic current with increasing concentration of the mediator as is observed in Figure 3. When the reaction between BQH, and ES, is the rate-determining step of reaction 2 , that is, when cw.…”

Section: Results and Discusson Eleclrocbemical Redm Reaction Of Bq Inmentioning

confidence: 99%

“…Curve (C) shows the difference between curves (B) and (A).the bioelectrocatalysis at an enzyme-modified electrode [ 1,[15][16][17]. According to a theory of bioelectrocatalysis[15][16][17],1, is given by 4' = nFAv,l( 6 ) where 1 is the thickness of the immobilized enzyme layer on the electrode surface, (BQH,) and (ES,) in u,(Equation 3) are the concentrations of BQH, and ES, in the enzyme layer, respectively. The former is in large excess (( BQH2) %-KL,J at the NOD electrode as stated above, and the latter can be expressed by [ 141:(7) Here KL,N is the apparent Michaelis constant for inosine inEquation 1and is a function of the concentration of 02.…”

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Nucleoside oxidase‐modified carbon paste electrode containing quinone: Cathodic current response to nucleosides

et al. 1991

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The cathodic current for the reduction of benzoquinone at a glassy carbon electrode was increased in the presence of nucleoside oxidase and inosine. This was attributed to the regeneration of benzoquinone from hydroquinone by the laccaselike reaction of the enzyme, the rate of which depended on the concentration of inosine in the solution. Accordingly, an enzyme electrode for nucleosides was constructed by immobilizing nucleoside oxidase behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone or hydroquinone. The current response of the electrode to nucleosides was studied, and the results were discussed on the basis of the laccaselike activity of the enzyme. The nucleoside oxidase-modified electrode was tested as an amperometric enzyme electrode for nucleosides and could be used at least for three weeks.k7W WORDS: Biosensors, nucleosides, nucleoside oxidase. A"TR0DUCTIONConsiderable attention has been given to the coupling of electrode reaction with an enzymatic reaction, in which the substrate of the enzyme can be oxidized or reduced electroenzymatically, i.e., by bioelectrocatalysis [ 1-31. Molecules serving as redox mediators are usually used to achieve the electron transfer coupling, and enzyme electrodes relying on this principle of current response, mediated amperometric enzyme electrodes, have been constructed (4, 51. A variety of redox molecules including redox polymers have been studied for the mediated electron transfer coupling based on glucose oxidase and other oxidoreductases [ 1-31. Similar coupling without mediators has also been reported between ordinary electrodes (such as carbon and metal surfaces), and several kinds of oxidoreductases [ 6-91.This article describes a new type of electroenzymatic coupling different from the above two coupling schemes, which rely on the unique properties of the enzyme used: nucleoside oxidase [NOD] from Pseudomom maltophiria LB-86 (10-131. NOD has a molecular weight of 'To whom correspondence should be addressed. 8 1992 VCH Publishers, Inc. 1040-0397/92/$3.50 + .25 891 892 Ikeda et al.

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Section: A Film-nod-cp(q)e As An Amperometricmentioning

confidence: 84%

Section: Results and Discusson Eleclrocbemical Redm Reaction Of Bq Inmentioning

confidence: 99%

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confidence: 99%

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Nucleoside oxidase‐modified carbon paste electrode containing quinone: Cathodic current response to nucleosides

et al. 1991

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“…is readily incorporated by using eq. (14), which relates 'cs to the concentration of S in the solution immediately adjacent to the s.m., *cS, I/ nFA = Ps.m(*cs_'lcs/ fls) (14) where Ps.m is the permeability of the s.m. to S and f3 S is the distribution coefficient of S between the e.l. and the solution.…”

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Theory of Catalytic Current at the Biocatalyst Electrode with Entrapped Mediator

1988

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A mathematical model of a biocatalyst electrode with entrapped mediator is presented. Differential equations describing the diffusion of the substrate and mediator coupled with the enzyme reaction in the immobilized enzyme layer adjacent to the electrode surface are numerically solved to simulate the dependence of the steady-state current, I, on the concentration of the substrate at the surface of the enzyme layer, ics, and on the concentration of the mediator entrapped in the enzyme layer, CM. The numerical solutions are expressed by Michaelis-Menten type equations with three parameters which characterize the I vs. acs and I'vs. CM curves. The diffusional resistance of a semipermeable membrane covering the enzyme layer on the electrode surface is also taken into consideration. The dependence of the current on the potential applied to the electrode, E, is also derived and the parameters characterizing the I vs. E curve are discussed.

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Bioelectrocatalytical Method for Glucose Determination in Foodstuffs

Stoytcheva¹

1992

Bulletin des Soc Chimique

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Biocatalyst electrodes and their use in electrochemical syntheses.

Cited by 8 publications

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Nucleoside oxidase‐modified carbon paste electrode containing quinone: Cathodic current response to nucleosides

Nucleoside oxidase‐modified carbon paste electrode containing quinone: Cathodic current response to nucleosides

Theory of Catalytic Current at the Biocatalyst Electrode with Entrapped Mediator

Bioelectrocatalytical Method for Glucose Determination in Foodstuffs

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