Bioelectrocatalytical Method for Glucose Determination in Foodstuffs

Stoytcheva, Margarita

doi:10.1002/bscb.19921011209

Cited by 1 publication

(3 citation statements)

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“…On the base of the presented results, advantageous electrochemical methods for the determination of enzyme substrates and inhibitors were developed, namely: -a method for glucose quantification in human blood serum [5] and in foodstuffs [7,8] in a large concentration range (10 -6 -1.6x10 -2 mol/L). It possesses the same exactitude as the conventional spectrophotometric ones (relative error 0.23-3.70 %), but it is more rapid; -a simple and rapid method for quinine quantification in soft drinks [16] with a low detection limit (10 -10 mol/L); -a method for AsO 3 3-quantification in waste waters [21].…”

Section: Discussionmentioning

confidence: 99%

“…(3) Mono-enzyme sensor of second generation with physically adsorbed glucose oxidase, for glucose quantification [4][5][6][7]. The enzyme immobilization was performed in static conditions, applying an optimized standard procedure.…”

Section: Biosensors Configurationsmentioning

confidence: 99%

“…Pt was used as auxiliary electrode and a saturated calomel electrode as a reference. The working electrode from spectrally pure graphite (Ringsdorf Werke, Germany) or from glassy carbon (Tokay GC 20 or Tacussel CTV 101 T), after a preliminary treatment consisting in polishing, degreasing with alcohol and ultrasonic cleaning, was modified applying different protocols for enzyme fixation, described in details in our previous works [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analytical Characteristics of Electrochemical Biosensors

Stoytcheva¹,

Zlatev²,

Velkova³

et al. 2009

Port. Electrochim. Acta

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The goal of this work is the evaluation of the analytical characteristics of the determinations performed using glucose oxidase and acetylcholinesterase based electrochemical sensors, developed applying original or optimized conventional methods of enzyme immobilization. It was found that the sensitivity of glucose determination, for example, varies from 0.048 to 3.36 mA L mol -1 cm -2 and the response time of the glucose oxidase based sensors -from 5 to 30 s, according to the method of the bioreceptor immobilization. The sensitivity of the analysis is affected from the activity of the immobilized biocomponent, from the composition of the solution (concentration of the substrate, of the mediator and of the inhibitor), and from the experimental conditions (pH, temperature, agitation), as well as from the kinetic parameters of the studied process. It was found that the immobilized glucose oxidase conserves its substrate specificity in the presence of a number of glucides (galactose, maltose, fructose, and saccharose) in 100 fold higher concentrations. The selectivity of glucose analysis is ensured applying a suitable potential. Interferences free glucose amperometric determination was performed at 0.00 V/SCE, in the presence of ascorbates and urates. The electrochemical quantification of enzyme inhibitors allows reaching particularly low limits of detection (10 -9 -10 -14 mol L -1 ).

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