Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide

Blattner, Christian; Zoumpanioti, Maria; Kröner, Jürgen; Schmeer, Georg; Xenakis, Aristotelis; Kunz, Werner

doi:10.1016/j.supflu.2005.06.007

Cited by 50 publications

(30 citation statements)

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“…These gels provide the advantage of a ''double'' immobilization of the enzyme, primary into the microemulsion droplets and secondly on the ''network'' of the gelling agent. They are stable in several organic polar or non-polar solvents (Delimitsou et al 2002;Blattner et al 2006) and are successfully used for esterification of fatty acids as well as phenolic acids with aliphatic alcohols (Delimitsou et al 2002;Zoumpanioti et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Esterification reactions catalyzed by lipases immobilized in organogels: effect of temperature and substrate diffusion

et al. 2008

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Rhizomucor miehei lipase was immobilized in hydroxy(propylmethyl) cellulose or agar gels containing lecithin or AOT microemulsions. The effect of the diffusion of substrates and products to this catalyst was studied, as well as the effect of temperature on the initial rate of ester synthesis. The composition of the gel affects the reaction rate due to mass transport phenomena. The apparent activation energies were higher for the systems based on agar, independently of the microemulsion used, and lower for the systems based on AOT microemulsions, independently of the polymer used.

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Section: Introductionmentioning

confidence: 99%

Esterification reactions catalyzed by lipases immobilized in organogels: effect of temperature and substrate diffusion

et al. 2008

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“…Recently, enzymatic reactions in microemulsions in scCO 2 have been reported in literature. [106][107][108] …”

Section: Methodsmentioning

confidence: 99%

Recent trends in whole cell and isolated enzymes in enantioselective synthesis

Milner¹,

Maguire²

2012

Arkivoc

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Modern synthetic organic chemistry has experienced an enormous growth in biocatalytic methodologies; enzymatic transformations and whole cell bioconversions have become generally accepted synthetic tools for asymmetric synthesis. This review details an overview of the latest achievements in biocatalytic methodologies for the synthesis of enantiopure compounds with a particular focus on chemoenzymatic synthesis in non-aqueous media, immobilisation technology and dynamic kinetic resolution. Furthermore, recent advances in ketoreductase technology and their applications are also presented.Keywords: Biocatalysis, kinetic and dynamic kinetic resolution, Baker's yeast, ketoreductases General IntroductionAsymmetric synthesis is the preferential formation of one stereoisomer of a chiral target compound to another; when scientists at GlaxoSmithKline, AstraZeneca and Pfizer 1 examined 128 syntheses from their companies, they found as many as half of the drug compounds made by their process research and development groups are not only chiral but also contain an average of two chiral centres each. 2In 2006 just 25 % were derived from the chiral pool and over 50 % employed chiral technologies. 1In order to meet regulatory requirements enantiomeric purities of 99.5 % were deemed necessary by the FDA. 3 This is one of the biggest challenges which face chemists today, primarily due to the recognition of the fact that different enantiomers of the same compound can interact differently in biological systems. As a consequence, the production of single enantiomers instead of racemic mixtures has become an important process in the pharmaceutical and agrochemical industry. Several routes can lead to the desired enantiomer including transition metal-, [4][5][6] organo-5,7-10 and bio-catalysis [11][12][13][14][15] and these have been thoroughly reviewed in the literature. 16,17 2. Biocatalysis IntroductionBiocatalysis involves the use of enzymes or whole cells (containing the desired enzyme or enzyme system) as catalysts for chemical reactions. A timeline of historic events in biocatalysis and biotechnology is outlined in Table 1. [18][19][20] Reviews and Accounts Novel methodologies for discovering industrial enzymes based on genomic sequencing and phage display (discussed further in Section 1.3), 21,22 as well as highly effective optimisation tools based on chemical, physical and molecular biology approaches, 23 have improved the access to biocatalysts, increased their stability, and radically broadened their specificity. 24This greater availability of catalysts with superior qualities including the use of new bioengineering tools contributed significantly to the development of new industrial processes. Biocatalytic methodologies for organic synthesis were outlined by Woodley et al. in three categories: established, emerging and expanding chemistries as depicted in Figure 1. 25 Enzyme classesBy the late 1950s it had become evident that the nomenclature of enzymes without any guiding authority, in a period when the...

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“…A lots of reports can be found in the literature about the application of micreomulsions prepared in supercritical fluids for different purposes, such as, investigation of sustained release of nucleic acids from polymeric nanoparticles prepared in sc-CO 2 microemulsions (Ge et al, 2010), biocatalysis using lipase encapsulated in microemulsion-based organogels in sc-CO 2 (Blattner et al, 2006), continuous tuning of size CdS and ZnS nanoparticles in a water-in-sc-CO 2 microemulsion (Fernandez & Wai, 2007), and synthesis of nanoporous clusters of zirconia (ZrO 2 ) (Lee et al, 2010).…”

Section: Microemulsions In Non-conventional Systemsmentioning

confidence: 99%

“…Among them is the delivering of genes into the cells (Gupta et al, 2004), with the aim of treatment of disease or diagnosis (Pedersen et al, 2006, Peng et al, 2006, Xu et al, 2011, Rossi et al, 2006, Santra et al, 2005, targeting cancer cell (Tao et al, 2011, Lu et al, 2008, Reithofer et al, 2011, act as vaccine (Sun et al, 2009, Mumper & Cui, 2003, biocatalyss (Stamatis & Xenakis, 1999, Zoumpanioti et al, 2010, Blattner et al, 2006, cosmetics (Chiu & Yang, 1992, Förster et al, 1995, Valenta & Schultz, 2004, Teichmann et al, 2007 or changing the genetic to improve the performance of the targeted cells , Courrier et al, 2004.…”

Section: Applications In Biological and Health Sciencesmentioning

confidence: 99%

Microemulsions - A Brief Introduction

Najjar¹

2012

Microemulsions - An Introduction to Properties and Applications

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Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide

Cited by 50 publications

References 50 publications

Esterification reactions catalyzed by lipases immobilized in organogels: effect of temperature and substrate diffusion

Esterification reactions catalyzed by lipases immobilized in organogels: effect of temperature and substrate diffusion

Recent trends in whole cell and isolated enzymes in enantioselective synthesis

Microemulsions - A Brief Introduction

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