Biocatalysis in organic media

Brink, L.E.S.; Tramper, J.; Luyben, K. Ch. A. M.; Riet, K. van ’t

doi:10.1016/0141-0229(88)90118-4

Cited by 152 publications

(31 citation statements)

References 54 publications

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“…This reversal of their original hydrolytic activity is usually obtained by using them in organic solvents at low water activity (Chulalaksananukul et al, 1990;Khmelnitsky et al, 1988) or in solventfree media composed only of the mixture of substrates (Bourg-Garros et al, 1998;Ergan et al, 1990;Fonteyn et al, 1994;Goldberg et al, 1988;Kim and Rhee, 1991). Biphasic media have also proven to be eective but are not very convenient (Brink et al, 1988;Luisi and Laane, 1986;Tweddell et al, 1998). This domain has been extensively studied, but as recently emphasized by Otera (2001), industry is always looking for practical esteri®-cation.…”

Section: Introductionmentioning

confidence: 98%

“…) has numerous applications:¯avors, emulsi®ers, stabilizing and conditioning molecules, fatty esters of polyols as drug carriers, surfactants in the detergent and cosmetic industries, and ecient processes for enantiomerically pure products in the pharmaceutical industry (Ballesteros et al, 1995;Brink et al, 1988;Dordick, 1990;Klibanov, 1985, 1988). This reversal of their original hydrolytic activity is usually obtained by using them in organic solvents at low water activity (Chulalaksananukul et al, 1990;Khmelnitsky et al, 1988) or in solventfree media composed only of the mixture of substrates (Bourg-Garros et al, 1998;Ergan et al, 1990;Fonteyn et al, 1994;Goldberg et al, 1988;Kim and Rhee, 1991).…”

Section: Introductionmentioning

confidence: 98%

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Esterification by immobilized lipase in solvent‐free media: Kinetic and thermodynamic arguments

Sandoval

Condoret

Monsan

et al. 2002

Biotech & Bioengineering

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The aim of this study is to characterize, in solvent-free systems (SFS), the kinetic and thermodynamic performance of batch lipase-catalyzed esterification. SFS are compared to a conventional organic solvent, n-hexane. The esterification of oleic acid with ethanol was chosen as a model reaction. The TABEK (thermodynamic activity-based enzyme kinetics) approach was used to rationally analyze kinetics. Influence of the reaction medium on final conversions was also studied. Several factors, such as initial molar ratio of substrates, reactant availability, initial water content, and quantity of immobilized enzyme, were examined. Special attention was also turned to enzyme stability and reuse after reaction, this last item being a prerequisite in the development of industrial processes. SFS proved to be almost as efficient as n-hexane from a kinetic and thermodynamic point of view and offered a better volumetric production.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Esterification by immobilized lipase in solvent‐free media: Kinetic and thermodynamic arguments

Sandoval

Condoret

Monsan

et al. 2002

Biotech & Bioengineering

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“…There is also facilitated recovery of products and biocatalyst [327]. As well in the presence of organic solvents there is less risk of microbial contamination [328]. In the specific case of laccases or LMS, many of their non-natural substrate are hardly soluble in water (their Km values are far away from their solubility in aqueous media), and therefore the use of cosolvents is an indispensable requirement.…”

Section: Potential New Laccase-based Biocatalystsmentioning

confidence: 99%

Laccases and Their Applications: A Patent Review

Kunamneni

Plou²,

Ballesteros³

et al. 2008

BIOT

272

172

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Abstract:Laccases are an interesting group of multi copper enzymes, which have received much attention of researchers in last decades due to their ability to oxidize both phenolic and non-phenolic lignin related compounds as well as highly recalcitrant environmental pollutants. This makes these biocatalysts very useful for their application in several biotechnological processes. Such applications include the detoxification of industrial effluents, mostly from the paper and pulp, textile and petrochemical industries, polymer synthesis, bioremediation of contaminated soils, wine and beverage stabilization. Laccases are also used as catalysts for the manufacture of anti-cancer drugs and even as ingredients in cosmetics. Recently, the utility of laccases has also been applied to nanobiotechnology. This paper reviews recent and important patents related to the properties, heterologous production, molecular cloning, and applications of laccases within different industrial fields as well as their potential extension to the nanobiotechnology area.

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“…Problems associated with low overall productivity (Habets-Criitzen et al, 1984) of the biocatalyst may be resolved by a) screening for more efficient systems where product degradation is minimal or absent and/or by b) process engineering, e.g., immobilization in multiphase reactors (Brink et al, 1988).…”

Section: Applications Of Epoxidation Reactions In Biotechnological Prmentioning

confidence: 99%

Biocatalysts for production of chiral epoxides

Mahmoudian

Michael

1992

Appl Microbiol Biotechnol

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An extensive screen for microorganisms capable of growth on olefins was carried out. Over 100 bacterial strains (mesophilic, thermophilic) were isolated in pure culture with gaseous or liquid (internal & terminal) olefins as the sole source of carbon and energy. The organisms included Aero coccus, Aeromonas,Alcaligenes, Flavobacterium, Micrococcus, Moraxella, Nocardia, Pseudomonas, Staphylococcus, Streptomyces and Vibrio spp. and a variety of Gram-negative, Gram-positive and Gram-variable rods/coccobacilli not yet identified. Given the success of isolating many different genera of alkene-utilizing bacteria, it would suggest that the ability to utilize olefin hydrocarbons is more widespread in nature than previously recognized.Initial studies involved testing a collection of strains for i) growth rate on the substrate of isolation and ii) evidence of epoxide accumulation from lower gaseous olefins. All of the 18 ethene-and propene-utilizing bacteria tested, stereospecifically formed R-1,2-epoxypropane (ee=90-96%), R -l,2-epoxybutane (ee=90-98%) and trans-(2/?,37?)-epoxybutane (ee=64-88%), as analyzed by chiral complexation glc. A particularly promising strain of Micrococcus sp. M90C was selected to demonstrate: a) Induction of epoxidation activity in carbohydrate-grown cells. b) Optimization of a biotransformation process for production of epoxyethane. c) Involvement of a specific monooxygenase in the formation of chiral epoxides. d) Stereoselectivity of epoxyalkane degradation. e) Substrate specificity toward a range of olefins.Micrococcus sp. M90C stereospecifically formed S-(+)-phenyl glycidyl ether (ee=93%) and /?-(+)-phenyl methyl sulphoxide (ee>98%), as analyzed by chiral-shift NMR and HPLC. The ability to catalyze heteroatom oxygenation as well as epoxidation of a wide range of olefins in high optical and chemical yields, demonstrates its potential as a 'general-purpose' biocatalyst for the production of optically pure oxygenated compounds.Two other promising ethene-utilizing bacteria (M26, M93 A) each catalyzed the stereospecific formation of S-(+)-phenyl glycidyl ether (ee=93%). This has attracted considerable industrial interest and is currently under investigation at ICI for commercial production of optically active./1-blockers. In addition to Micrococcus sp. M90C, the substrate specificities of 6 other organisms were studied in order to probe the active site of the enzymes involved. The pattern of reactivity for the group of 4 ethene-(M26, M90C, M93A, M186) and 2 propene-(M142, M l 56) utilizers differed from that expected with peracids, suggesting a considerable degree of enzymatic control.The nature of the substrate, however, modulated enzymatic epoxidations in Staphylcoccus sp. M97B. CONTENTS

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Biocatalysis in organic media

Cited by 152 publications

References 54 publications

Esterification by immobilized lipase in solvent‐free media: Kinetic and thermodynamic arguments

Esterification by immobilized lipase in solvent‐free media: Kinetic and thermodynamic arguments

Laccases and Their Applications: A Patent Review

Biocatalysts for production of chiral epoxides

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