Bioavailable flavonoids to suppress the formation of 8-OHdG in HepG2 cells

Kanazawa, Kazuki; Uehara, Mari; Yanagitani, Hiroaki; Hashimoto, Takashi

doi:10.1016/j.abb.2006.09.003

Cited by 84 publications

(47 citation statements)

References 46 publications

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“…Such lesions are thought to be strongly implicated in cancer initiation [66]. Quercetin and luteolin at 10 mM were able to significantly suppress the formation of the DNA oxidative damage marker, 8-oxo-7,8-dihydrodeoxyguanosine, by ROS in cell cultures, suggesting an ability to limit genetic damage [67]. Attempts to reproduce these indirect antioxidant effects in vivo have produced relatively unconvincing results.…”

Section: Indirect Antioxidant Capacity -Activation Of Endogenous Antimentioning

confidence: 99%

Polyphenolic phytochemicals – just antioxidants or much more?

Stevenson

Hurst

2007

Cell. Mol. Life Sci.

478

323

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Polyphenolic phytochemicals are ubiquitous in plants, in which they function in various protective roles. A 'recommended' human diet contains significant quantities of polyphenolics, as they have long been assumed to be 'antioxidants' that scavenge excessive, damaging, free radicals arising from normal metabolic processes. There is recent evidence that polyphenolics also have 'indirect' antioxidant effects through induction of endogenous protective enzymes. There is also increasing evidence for many potential benefits through polyphenolic-mediated regulation of cellular processes such as inflammation. Inductive or signalling effects may occur at concentrations much lower than required for effective radical scavenging. Over the last 2-3 years, there have been many exciting new developments in the elucidation of the in vivo mechanisms of the health benefits of polyphenolics. We summarise the current knowledge of the intake, bio-availability and metabolism of polyphenolics, their antioxidant effects, regulatory effects on signalling pathways, neuro-protective effects and regulatory effects on energy metabolism and gut health.

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Section: Indirect Antioxidant Capacity -Activation Of Endogenous Antimentioning

confidence: 99%

Polyphenolic phytochemicals – just antioxidants or much more?

Stevenson

Hurst

2007

Cell. Mol. Life Sci.

478

323

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“…Human liver HepG2 is widely used for biochemical and nutritional studies as a cell culture model in human hepatocytes since they retain their morphology and most of their function in culture (Brandon et al, 2006;Goya, Martín, Ramos, Mateos, & Bravo, 2009). Previous cell culture studies have demonstrated that several flavonoids (Kanazawa, Uehara, Yanahitani, & Hashimoto, 2006) and hydroxycinnamic acids are absorbed and metabolized by HepG2 cells. These cells are able to express main antioxidant defense enzymes such as catalase, glutathione peroxidase (GPx), glutathione reductase (GR) on par with or better than that of primary human hepatocytes (Lee et al, 2002;Mersch-Sundermann, Knasmuller, Wu, Darroudi, & Kassie, 2004).…”

Section: Introductionmentioning

confidence: 99%

Chemical characterization and chemo-protective activity of cranberry phenolic powders in a model cell culture. Response of the antioxidant defenses and regulation of signaling pathways

Martín

Ramos

Mateos

et al. 2015

Food Research International

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“…Different cell culture studies have demonstrated that diverse flavonoids (18), the major olive oil phenols, hydroxytyrosol (HTy) and hydroxytyrosyl acetate (19), and several hydroxycinammic acids (20) are absorbed and metabolized by HepG2 cells in culture.…”

Section: Introductionmentioning

confidence: 99%

Protection of Human HepG2 Cells against Oxidative Stress by Cocoa Phenolic Extract

Martín

Ramos

Mateos

et al. 2008

J. Agric. Food Chem.

107

110

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Cocoa is a rich source of flavanols and procyanidin oligomers with antioxidative properties, providing protection against oxidation and nitration. The present study investigated the potential protective effect of a polyphenolic extract from cocoa on cell viability and antioxidant defenses of cultured human HepG2 cells submitted to oxidative stress induced by tert-butylhydroperoxide (t-BOOH). Pretreatment of cells with 0.05-50 µg/mL of cocoa polyphenolic extract (CPE) for 2 or 20 h completely prevented cell damage and enhanced activity of antioxidant enzymes induced by a treatment with t-BOOH. Moreover, lower levels of GSH caused by t-BOOH in HepG2 cells were partly recovered by a pretreatment with CPE. Increased reactive oxygen species (ROS) induced by t-BOOH was dosedependently prevented when cells were pretreated for 2 or 20 h with CPE. These results show that treatment of HepG2 in culture with CPE (within the physiological range of concentrations) confers a significant protection against oxidation to the cells.

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Bioavailable flavonoids to suppress the formation of 8-OHdG in HepG2 cells

Cited by 84 publications

References 46 publications

Polyphenolic phytochemicals – just antioxidants or much more?

Polyphenolic phytochemicals – just antioxidants or much more?

Chemical characterization and chemo-protective activity of cranberry phenolic powders in a model cell culture. Response of the antioxidant defenses and regulation of signaling pathways

Protection of Human HepG2 Cells against Oxidative Stress by Cocoa Phenolic Extract

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