Bioavailability of cellobiose by tolerance test and breath hydrogen excretion in humans

Nakamura, Shin; Oku, Tsuneyuki; Ichinose, Makoto

doi:10.1016/j.nut.2004.08.005

Cited by 48 publications

(40 citation statements)

References 13 publications

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“…Most of these bacteria harbor several loci in common with the cellobiose-specific PTS, implying the ability to metabolize ␤-glucoside sugars (42). digestible oligosaccharides escape from digestion in the small intestine, enter the colon, and are catalyzed by the microbes that reside there (33). Presumably, celB could facilitate the utilization of cellobiose by K. pneumoniae in this environment, providing this species with a potential competitive advantage.…”

Section: Discussionmentioning

confidence: 99%

Cellobiose-Specific Phosphotransferase System of Klebsiella pneumoniae and Its Importance in Biofilm Formation and Virulence

Chen

Lin

et al. 2012

Infect Immun

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ABSTRACTKlebsiella pneumoniaeis a Gram-negative bacillus belonging to the familyEnterobacteriaceae. In the past 20 years,K. pneumoniaehas become the predominant pathogen causing community-acquired pyogenic liver abscess (PLA). The formation of biofilm facilitates bacterial colonization and has been implicated in reduced susceptibility to the host immune response. To investigate genes related to biofilm formation in a PLA-associatedK. pneumoniaestrain, a transposon mutant library was screened by microtiter plate assay to identify isolates impaired for biofilm formation. One of the mutants was disrupted incelB, encoding the putative cellobiose-specific subunit IIC of enzyme II (EIIC) of a carbohydrate phosphotransferase system (PTS). This transmembrane protein is responsible for recognizing and binding specific sugars and transporting them across the cell membrane into the cytoplasm. Deletion and chromosomal complementation ofcelBconfirmed, by microtiter plate and slide culture assays, thatcelBwas indeed responsible for biofilm formation. Cellobiose-specific PTS activities of deletion mutants grown in LB broth and 0.005% cellobiose minimal medium were markedly lower than that of the wild-type strain grown under the same conditions, thereby confirming the involvement ofcelBin cellobiose transport. In 0.005% cellobiose minimal medium, thecelBmutant showed a delay in growth compared to the wild-type strain. In a mouse model of intragastric infection, deletion of thecelBgene increased the survival rate from 12.5% to 87.5%, which suggests that thecelBdeletion mutant also exhibited reduced virulence. Thus, thecelBlocus ofK. pneumoniae may contribute to biofilm formation and virulence through the metabolism of cellobiose.

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Section: Discussionmentioning

confidence: 99%

Cellobiose-Specific Phosphotransferase System of Klebsiella pneumoniae and Its Importance in Biofilm Formation and Virulence

Chen

Lin

et al. 2012

Infect Immun

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“…Enzymes capable of breaking down cellobiose are absent in the human small intestine. Nakamura et al [4] confirmed that cellobiose may be hydrolyzed slowly by intestinal lactase in an in vitro study with rat small intestinal brush border membrane vesicles. There are some reports of new classes of sugars, containing cellobiose as a component, produced by transglycosylation reactions [5,6].…”

Section: Introductionmentioning

confidence: 92%

Optimization of Oligosaccharide Synthesis from Cellobiose by Dextransucrase

Kim

Day

2007

Appl Biochem Biotechnol

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There is a growing market for oligosaccharides as sweeteners, prebiotics, anticariogenic compounds, and immunostimulating agents in both food and pharmaceutical industries. Interest in novel carbohydrate-based products has grown because of their reduced toxicity and low immune response. Cellobiose is potentially valuable as a nondigestible sugar. The reaction of cellobiose, as an acceptor with a sucrose as a donor, catalyzed by a dextransucrase from Leuconostoc mesenteroides B-512FMCM, produced a series of cellobio-oligosaccharides. This production system was optimized using a Box-Behnken experimental design for 289 mM of sucrose and 250 mM of cellobiose and 54 U of the enzyme at pH 5.2 and 30 degrees C, to produce maximum yields of oligosaccharide.

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“…This study was designed as a singleblinded, placebo-controlled study, and was carried out as described in our previous study [8,29,30], under the direction of a medical doctor.…”

Section: Experimental Protocolmentioning

confidence: 99%

Hypoglycemic Effects of Morus alba Leaf Extract on Postprandial Glucose and Insulin Levels in Patients with Type 2 Diabetes Treated with Sulfonylurea Hypoglycemic Agents

Nakamura¹,

Hashiguchi²,

Yamaguchi³

et al. 2011

J Diabetes Metab

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Bioavailability of cellobiose by tolerance test and breath hydrogen excretion in humans

Cited by 48 publications

References 13 publications

Cellobiose-Specific Phosphotransferase System of Klebsiella pneumoniae and Its Importance in Biofilm Formation and Virulence

Cellobiose-Specific Phosphotransferase System of Klebsiella pneumoniae and Its Importance in Biofilm Formation and Virulence

Optimization of Oligosaccharide Synthesis from Cellobiose by Dextransucrase

Hypoglycemic Effects of Morus alba Leaf Extract on Postprandial Glucose and Insulin Levels in Patients with Type 2 Diabetes Treated with Sulfonylurea Hypoglycemic Agents

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