Bioavailability: Is this a key event in regulating the actions of peptide growth factors?

Logan, A.; Hill, D. J.

doi:10.1677/joe.0.1340157

Cited by 37 publications

(13 citation statements)

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“…The ECM proteins may serve as storage sites for many growth factors [35], and bFGF has been detected in uterine ECM of rodents [12]. Matrix proteins in the uterus turn over cyclically and during pregnancy, thus making them particularly good candidates for local control of the uterine environment in response to matrix-released growth factors [12].…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization of Acidic and Basic Fibroblast Growth Factors in Porcine Uterine and Conceptus Tissues1

Gupta

Bazer

Jaeger

1997

Biology of Reproduction

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Immunohistochemical techniques were used to localize acidic and basic fibroblast growth factor (FGF) polypeptides in porcine uterine and conceptus tissues collected on Days 10 through 14 of gestation, which is the peri-implantation period, and in uterine tissues collected on the same days of the estrous cycle. Our results demonstrate differential expression of acidic and basic FGF (aFGF, bFGF) in porcine uterine and conceptus tissues. Localization of these peptides in the uterus of cycling and pregnant pigs was different from that reported for other species, suggesting species-specific roles for FGFs in early pregnancy. Increases in both cytoplasmic and nuclear bFGF immunostaining were detected in uterine luminal and glandular epithelial cells from Days 10 to 14 of gestation but not in the uterine epithelium of cycling pigs. Acidic FGF immunostaining was not detected in luminal or glandular epithelium of either cycling or pregnant uterine tissue; however, differential stromal staining was observed. Uterine tissues collected from pigs on Days 10-14 of the estrous cycle had diffuse aFGF immunostaining throughout the stroma. During early pregnancy, however, intense aFGF immunostaining was concentrated around the glandular epithelial and below the luminal epithelial cells as gestation progressed. Basic FGF, but not aFGF, was detected in porcine conceptuses collected during the peri-implantation period (Days 10-14 of gestation). Although both acidic and basic FGF belong to the same family of proteins, results of the present study indicate that they likely play different roles in uterine function and conceptus development in pigs.

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Section: Discussionmentioning

confidence: 99%

Immunolocalization of Acidic and Basic Fibroblast Growth Factors in Porcine Uterine and Conceptus Tissues1

Gupta

Bazer

Jaeger

1997

Biology of Reproduction

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“…In mice and rhesus monkeys, MMPs are up-regulated during the transformation of follicular granulosa cells into functional luteal tissue, and again during luteal regression (Young and Stouffer, 2003;Liu et al, 1999). In addition to physical remodeling, the cleavage action of ovarian MMPs can release growth factors in vitro encouraging follicular development, and in turn, ovulation (Levi et al, 1996;Fowlkes et al, 1994;Gearing et al, 1995;Logan and Hill, 1992;Massova et al, 1998;Stouffer et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Differential expression of matrix metalloproteinases during stimulated ovarian recrudescence in Siberian hamsters (Phodopus sungorus)

Salverson

McMichael

Sury

et al. 2008

General and Comparative Endocrinology

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The matrix metalloproteinases (MMPs) are a family of extracellular matrix-cleaving enzymes involved in ovarian remodeling. In many non-tropical species, including Siberian hamsters, ovarian remodeling is necessary for the functional changes associated with seasonal reproduction. We evaluated MMPs and their endogenous inhibitors (TIMPs), during photoperiod-induced ovarian recrudescence in Siberian hamsters. Hamsters were transferred from long-day (LD;16:8) to shortday (SD;8:16) photoperiods for 14wks, and then returned to LD for 0,1,2,4, or 8wks for collection of ovaries and plasma. Post-transfer (PT) LD exposure increased body and ovarian mass. Numbers of corpora lutea and antral, but not preantral follicles increased in PT groups. Plasma estradiol concentrations were lower in PT wks0−4, and returned to LD levels at PTwk8. No change was observed in relative MMP/TIMP mRNA levels at PTwk0 (SDwk14) as compared to LD. Photostimulation increased MMP-2 mRNA at PTwk8 as compared to PT wks 0−1. MMP-14 mRNA expression peaked at PTwks1−2 as compared to LD levels, while MMP-13 expression was low during this time. TIMP-1 mRNA peaked at PT wk8 as compared to PTwks0−4. No changes were noted in MMP-9 and TIMP-2 mRNA expression. In general, MMP/TIMP protein immunodetection followed the same patterns with most staining occurring in granulosa cells of follicles and corpora lutea. Our data suggest that mRNA and protein for several members of the MMP/TIMP families are expressed in Siberian hamster ovaries during recrudescence. Because of the variation observed in expression patterns, MMPs and TIMPs may be differentially involved with photo-stimulated return to ovarian function.

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“…Many growth factors bind to this substrate, and it has been postulated that the matrix serves as a sequestration site from which growth factor stores can be concentrated for enhanced local action or released for heightened overall effect (1)(2)(3)(4)(5)(6)(7). Basic fibroblast growth factor (bFGF) 1 is a model compound in this regard.…”

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confidence: 99%

Cellular Response to Transforming Growth Factor-β1 and Basic Fibroblast Growth Factor Depends on Release Kinetics and Extracellular Matrix Interactions

Dinbergs

Brown

Edelman

1996

Journal of Biological Chemistry

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The extracellular matrix plays an important role in growth factor biology, serving as a potential platform for rapid growth factor mobilization or a sink for concentrated sequestration. We now demonstrate that when a growth factor binds reversibly to the matrix, its effects are augmented by this interaction, and when the factor is absorbed irreversibly to the extracellular matrix, it becomes sequestered. These findings call into question the notion that all growth factors are best presented to cells and tissues in a sustained and controlled fashion. In our studies, we examined basic fibroblast growth factor (bFGF) and transforming growth factor-␤1 (TGF-␤1) release kinetics from synthetically fabricated microsphere devices and naturally synthesized extracellular matrix. While the sustained release of bFGF was up to 3.0-fold more potent at increasing vascular endothelial and smooth muscle cell proliferation than bolus administration, the reverse was true for TGF-␤1. A bolus of TGF-␤1 inhibited vascular cells up to 3.8-fold more efficiently than the same amount of TGF-␤1 if control-released. Both growth factors bound to the extracellular matrix, but only bFGF was released in a controlled fashion (2.8%/day). Contact with the extracellular matrix and subsequent release enhanced bFGF activity such that it was 86% more effective at increasing smooth muscle cell numbers than equal amounts of growth factor diluted from frozen stock. TGF-␤1 remained tightly adherent. The small amount of TGF-␤1 released from the extracellular matrix was ϳ30% less effective than bolus administration at inhibiting vascular endothelial and smooth muscle cell growth. Sustained growth factor release may be the preferable mode of administration, but only when a similar mode of metabolism is utilized endogenously.

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Bioavailability: Is this a key event in regulating the actions of peptide growth factors?

Cited by 37 publications

References 0 publications

Immunolocalization of Acidic and Basic Fibroblast Growth Factors in Porcine Uterine and Conceptus Tissues1

Immunolocalization of Acidic and Basic Fibroblast Growth Factors in Porcine Uterine and Conceptus Tissues1

Differential expression of matrix metalloproteinases during stimulated ovarian recrudescence in Siberian hamsters (Phodopus sungorus)

Cellular Response to Transforming Growth Factor-β1 and Basic Fibroblast Growth Factor Depends on Release Kinetics and Extracellular Matrix Interactions

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