Bioaugmentation of DDT-contaminated soil by dissemination of the catabolic plasmid pDOD

Gao, Chunming; Jin, Xiangxiang; Ren, Jie; Fang, Hua; Yu, Yunlong

doi:10.1016/j.jes.2014.05.045

Cited by 20 publications

(6 citation statements)

References 28 publications

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“…In a contaminated field site located in Cixi, Zhejiang (China), Gao et al (2015) achieved effective plasmid-mediated bioaugmentation for the degradation of dichlorodiphenyltrichloroethane (DDT) in soil with E. coli TG I (pDOD-gfp) as donor strain. In this study, the catabolic plasmid pDOD from Sphingobacterium sp.…”

Section: Genetic (Plasmid-mediated) Bioaugmentationmentioning

confidence: 99%

“…D-6 was conjugally transferred to soil bacteria, such as members of Cellulomonas , and accelerated DDT degradation. Different studies have reported the use of the GFP (green fluorescence protein) detection system to monitor plasmid transfer from donor cells to indigenous soil bacteria in soil slurries ( Ikuma et al, 2012 ) and field contaminated soil ( Gao et al, 2015 ).…”

Section: Genetic (Plasmid-mediated) Bioaugmentationmentioning

confidence: 99%

“…Soil moisture can have an effect on plasmid transfer during plasmid-mediated bioaugmentation by affecting the contact between donor and recipient bacteria ( Miller et al, 2004 ; Aminov, 2011 ). In this respect, Gao et al (2015) evaluated the effectiveness of plasmid-mediated bioaugmentation for p,p′ -DDT degradation at three different soil moisture conditions (40, 60, and 80%), and concluded that 60% moisture content was optimal for maximum plasmid transfer efficiency.…”

Section: Influence Of Abiotic and Biotic Factors On Bioaugmentationmentioning

confidence: 99%

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Plasmid-Mediated Bioaugmentation for the Bioremediation of Contaminated Soils

et al. 2017

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Bioaugmentation, or the inoculation of microorganisms (e.g., bacteria harboring the required catabolic genes) into soil to enhance the rate of contaminant degradation, has great potential for the bioremediation of soils contaminated with organic compounds. Regrettably, cell bioaugmentation frequently turns into an unsuccessful initiative, owing to the rapid decrease of bacterial viability and abundance after inoculation, as well as the limited dispersal of the inoculated bacteria in the soil matrix. Genes that encode the degradation of organic compounds are often located on plasmids and, consequently, they can be spread by horizontal gene transfer into well-established, ecologically competitive, indigenous bacterial populations. Plasmid-mediated bioaugmentation aims to stimulate the spread of contaminant degradation genes among indigenous soil bacteria by the introduction of plasmids, located in donor cells, harboring such genes. But the acquisition of plasmids by recipient cells can affect the host’s fitness, a crucial aspect for the success of plasmid-mediated bioaugmentation. Besides, environmental factors (e.g., soil moisture, temperature, organic matter content) can play important roles for the transfer efficiency of catabolic plasmids, the expression of horizontally acquired genes and, finally, the contaminant degradation activity. For plasmid-mediated bioaugmentation to be reproducible, much more research is needed for a better selection of donor bacterial strains and accompanying plasmids, together with an in-depth understanding of indigenous soil bacterial populations and the environmental conditions that affect plasmid acquisition and the expression and functioning of the catabolic genes of interest.

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Section: Genetic (Plasmid-mediated) Bioaugmentationmentioning

confidence: 99%

Section: Genetic (Plasmid-mediated) Bioaugmentationmentioning

confidence: 99%

Section: Influence Of Abiotic and Biotic Factors On Bioaugmentationmentioning

confidence: 99%

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Plasmid-Mediated Bioaugmentation for the Bioremediation of Contaminated Soils

et al. 2017

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“…Biodegradation has been considered as a cost-effective, safe, and promising method for the removal or detoxification of DDT residues in the environment 8 9 . The degradation characteristics of DDT by microorganisms have been well documented, and some DDT-degrading microorganisms have been isolated, such as Alcaligenes eutrophus A5, Alcaligenes sp.…”

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confidence: 99%

Biodegradation of DDT by Stenotrophomonas sp. DDT-1: Characterization and genome functional analysis

Pan

Lin

Zheng

et al. 2016

Sci Rep

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A novel bacterium capable of utilizing 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) as the sole carbon and energy source was isolated from a contaminated soil which was identified as Stenotrophomonas sp. DDT-1 based on morphological characteristics, BIOLOG GN2 microplate profile, and 16S rDNA phylogeny. Genome sequencing and functional annotation of the isolate DDT-1 showed a 4,514,569 bp genome size, 66.92% GC content, 4,033 protein-coding genes, and 76 RNA genes including 8 rRNA genes. Totally, 2,807 protein-coding genes were assigned to Clusters of Orthologous Groups (COGs), and 1,601 protein-coding genes were mapped to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. The degradation half-lives of DDT increased with substrate concentration from 0.1 to 10.0 mg/l, whereas decreased with temperature from 15 °C to 35 °C. Neutral condition was the most favorable for DDT biodegradation. Based on genome annotation of DDT degradation genes and the metabolites detected by GC-MS, a mineralization pathway was proposed for DDT biodegradation in which it was orderly converted into DDE/DDD, DDMU, DDOH, and DDA via dechlorination, hydroxylation, and carboxylation, and ultimately mineralized to carbon dioxide. The results indicate that the isolate DDT-1 is a promising bacterial resource for the removal or detoxification of DDT residues in the environment.

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“…Bioaugmentation. A plasmid transfermediated bioaugmentation method for the enhancement of dichloro diphenyl trichloroethane (DDT) degradation in soil was developed byGao et al (2015) using the catabolic plasmid pDOD from Sphingobacterium sp. D-6.…”

mentioning

confidence: 99%