Bioassay of Industrial Waste Pollutants

Selivanovskaya, Svetlana; Hung, Yung‐Tse; Stepanova, Nadezda Yu.; Латыпова, В. З.

doi:10.1201/9780849375750.ch2

Cited by 2 publications

(4 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This analytical approach does not allow for mixture toxicity, nor does it take into account the bioavailability of the pollutants present. In this respect, bioassays provide an alternative because they constitute a measure for environmentally relevant toxicity, that is, the effects of a bioavailable fraction of an interacting set of pollutants in a complex environmental matrix [4,6] In this study, an attempt was made to determine effects of aromatic hydrocarbons and marine sediments from Niger Delta on the βgalactosidase activity of mutant E. coli and the result in Table 1 showed that the detectable hydrocarbons values were above the WHO standard for PAHs (50 ng /L) in water. The reason may be due to the hydrophobic and insoluble nature of aromatic hydrocarbons to water molecules making them to adsorb more on the surface of sediments than water samples.…”

Section: Discussionmentioning

confidence: 99%

“…Following the methods of Aruoja et al [15] and Selivanovskaya et al [4] with slight modifications, the sediment-water suspensions (10:100 w/v) were prepared by shaking the dry sediments with sterile distilled water for 24 hrs at room temperature (25 ± 2°C).…”

Section: Sample Preparation and Extractionmentioning

confidence: 99%

“…Ecological risk assessment (ERA) is a tool to estimate adverse effects on the environment from chemical or physical stressors. Toxicity bioassays are the important line of evidence in an ERA [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…The variables measured in toxicity tests may be lethality, growth rate, change in species diversity, decrease in degradation activity, and energy metabolism or activity of specific enzymes. The results are generally expressed as the doseresponse concentration and the EC 50 or EC 10 value [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of Aromatic Hydrocarbons and Marine Water from Niger Delta on the β-Galactosidase Activity of Mutant Escherichia coli

Uba

2019

ACRI

View full text Add to dashboard Cite

Aims:To determine effects of aromatic hydrocarbons and marine water from Niger Delta on the β-Galactosidase activity mutant Escherichia coli. Study Design: Fifteen treatments and the control designs were set up in triplicates in microtitre plates containing 200 µL of the 100% concentration of samples (three marine waters and distilled water spiked each with xylene, anthracene and pyrene). The fifteen treatments and control (HgCl 2 ) set ups designated as A, B, C, D, E, F and G were used to determine their median effective concentration (EC 50 ) for the inhibition of β-Galactosidase activity of mutant Escherichia coli. Place and Duration of Study: Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli Nigeria between February, 2017 to July, 2017. Methodology: A laboratory scale study was carried on the water samples from the three studied areas using physicochemical analyses and bacterial Toxi-chromo test. Results: The findings revealed that the three sampling sites contain higher quantities of aromatic hydrocarbons, heavy metals and other physico-chemical parameters in the sediment samples than water samples. Xylene distilled water had the highest EC 50 of 3.417 ± 0.094 mg/l while pyrene Onne water had the least EC 50 of 0.015 ± 0.002 mg /L. Also, pyrene and anthracene are significantly (P = .05) highly toxic aromatic hydrocarbons (EC 50 < 1 mg /L) while xylene is a Original Research Article ArticleUba; ACRI, 16(3): 1-16, 2019; Article no.ACRI.46679 2 significantly (P = .05) toxic aromatic hydrocarbon (1 mg /L < EC 50 ≤ 10 mg /L) compare to the positive control (HgCl 2 ) (EC 50 < 1 mg /L) indicating that enzyme inhibition among test samples were much different from the positive control. Conclusion: Thus, the toxicity results (< 0.1 mg /L < EC 50 ≤ 10 mg /L) in this study indicate that the potential eco-toxicity and environmental health effects of these toxicants should be given attention in order to get rid of their dangerous outcomes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Sample Preparation and Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%