Bioassay-guided purification, GC-MS characterization and quantification of phyto-components in an antibacterial extract of Searsia lancea leaves

Abstract

Phytocompounds in an aqueous methanol (70% MeOH) leaf extract of Searsia lancea were separated using liquid-liquid partitioning techniques and gravity-assisted column chromatography. The resultant fractions were screened for antibacterial properties (minimum inhibitory concentration, MIC) against four bacterial strains (Enterococcus faecalis, Klebsiella pneumoniae, Neisseria gonorrhoeae and Staphylococcus aureus). Bioactive fractions were purified using preparative thin layer chromatography (TLC) and subjected to further antibacterial screening. Phytocompounds in antibacterial sub-fractions were characterized and quantified using Gas Chromatography-Mass Spectrometry (GC-MS). An ethyl acetate sub-fraction purified from the aqueous methanol extracts of the leaves demonstrated potent antibacterial properties (MIC range: 31-61 µg/ml against E. faecalis and S. aureus). Based on GC-MS analysis, 81.5% of the sub-fraction consisted of broad-spectrum antibacterial compounds namely tetracosanol (43.98%) and nonadecanol (37.5%). Current research findings support the traditional use of S. lancea leaves to manage gastro-intestinal disorders and gonorrhoea.

Physicochemical Characterization and Antimicrobial Activity against Erwinia amylovora, Erwinia vitivora, and Diplodia seriata of a Light Purple Hibiscus syriacus L. Cultivar

“…As regards other constituents that were not assayed in vitro, hexadecanoic acid and its esters are considered antifungals and antioxidants [ 47 ]. The same applies to 1-tetracosanol [ 48 , 49 ], and to the unsaturated linolenic and linoleic fatty acids [ 50 , 51 ]. Moreover, according to Čechovská et al [ 40 ], part of the antioxidant activity of H. syriacus flowers can be ascribed to 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one or DDMP-4-one, and such antioxidant activity is generally associated with antibacterial, antifungal, and antimycotoxigenic biological activities [ 52 ].…”

Proliferative Effect of Aqueous Extract of Sea Cucumber (Holothuria parva) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells

“…The 3D plot of the binding sites and the 2D plot of interactions of Cyclin E with (C) 1, 2-benzene dicarboxylic acid, diisooctyl ester, and (D) 3,4-Dihydro-1h-isoquinoline-2-carboxamidine hydrochloride, Figure S6: The 3D plot of the binding sites and the 2D plot of interactions of HIF-1α with (A) 6,10-Dimethyldodeca-5,9-dien-2-one, and (B) 1, 2-benzene dicarboxylic acid, diisooctyl ester. The 3D plot of the binding sites and the 2D plot of interactions of TERT with (C) 1, 2-benzene dicarboxylic acid, diisooctyl ester, and (D) 3,4-Dihydro-1h-isoquinoline-2-carboxamidine hydrochloride, Figure S7: The 3D plot of the binding sites and the 2D plot of interactions of p21 with (A) 3,4-Dihydro-1h-isoquinoline-2-carboxamidine hydrochloride, (B) Azulene, (C) Benzene 1-ethyl-3-methyl-, (D) D-glucose, cyclic ethylene mercaptan, pentaacetate, and E) Phenol,2,4-bis(1,1dimethylethyl)-, Figure S8: The 3D plot of the binding sites and the 2D plot of interactions of PCNA with (A) (5E,9E)-6,10-Dimethyldodeca-5,9-dien-2-one, (B) 3,4-Dihydro-1h-isoquinoline-2-carboxamidine hydrochloride, (C) Benzene 1-ethyl-3-methyl-, (D) Cyclooctaneacetic acid, 2-oxo-, and (E) Cycloundecene,1-methyl-, Figure S9: The original gels of western blot analysis, Table S1: GC-MS compounds in aqueous extract of sea cucumber ( Holothuria parva ) based on PubChem database, Table S2: The values of binding affinity (Kcal/mol) of ligands to receptors [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ,…”