Bioassay-guided isolation of anti-inflammatory, antinociceptive and wound healer glycosides from the flowers of Verbascum mucronatum Lam.

Akdemir, Zeliha Ş.; Kahraman, Cigdem; Tatlı, I. İrem; Akkol, Esra Küpeli; Süntar, İpek; Keleş, Hikmet

doi:10.1016/j.jep.2010.05.059

Cited by 70 publications

(59 citation statements)

References 17 publications

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“…Our results supported the previous findings that verbascoside reduces the production of superoxide radicals (Speranza et al, 2010), exerts exquisite corticosteroid-like inhibition of proinflammatory chemokines (Georgiev et al, 2012) and is a specific inhibitor of paw edema (Akdemir et al, 2011) which explain its beneficial effect in the treatment of inflammatory diseases. The absence of this compound in the mullein extract is a possible hypothesis for the failure in exhibiting a certain anti-inflammatory effect.…”

Section: Discussionsupporting

confidence: 92%

Correlation between polyphenol content and anti-inflammatory activity ofVerbascum phlomoides(mullein)

Grigore

Colceru-Mihul

Liţescu

et al. 2013

Pharmaceutical Biology

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Context: Verbascum phlomoides L. (Scrophulariaceae) (mullein) used in the European folk medicine due to its anti-inflammatory and soothing action on the respiratory tract is thoroughly documented in handbooks and scientific literature. Nevertheless, information regarding the influence of the polyphenol content on pharmacological activity is scarce. Objective: This study explored the antioxidant and anti-inflammatory potential of V. phlomoides polyphenol-rich extract. Materials and methods: Dried mullein flowers (200 g) were subjected to water extraction (60 C, 2 h, herb/solvent ratio ¼ 1/10 m/v) and further to n-butanol partition. Total phenolics were spectrophotometrically determined and specific compounds were evaluated by HPLC. The antioxidant activity was assessed by the 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) assay. The anti-inflammatory potential of the extract (50-200 mg/mL) was evaluated in vitro by ELISA measurement of ICAM-1 expression in TNF-a-stimulated endothelial cells and in vivo by the rat paw edema assay. Results: The mullein extract contained 4.18% total polyphenols expressed as gallic acid. The main components identified by HPLC were: rosmarinic acid (14.93 mg/g), caffeic acid (39.96 mg/g), ferulic acid (29.61 mg/g) and quercetin (17.29 mg/g). Acteoside was not detected; aucubin was detected in traces (0.028 mg/g). Depending on concentration, the extract exerted scavenging activity on DPPH radical (EC 50 7.09 mg/mL), significantly inhibited TNF-a-induced ICAM-1 expression by 55-58.8% on human umbilical vein endothelial cells at 100 and 200 mg/mL, but failed to reduce egg-white-induced rat paw edema. Discussion and conclusion: Mullein polyphenols play an important role in exerting the antioxidant effect but have a weak influence on anti-inflammatory activity that is correlated, probably, to a higher content of iridoids and phenylethanoids.

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Section: Discussionsupporting

confidence: 92%

Correlation between polyphenol content and anti-inflammatory activity ofVerbascum phlomoides(mullein)

Grigore

Colceru-Mihul

Liţescu

et al. 2013

Pharmaceutical Biology

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“…In vitro, compound 121 not only inhibited the release of HMGB1, the production of inducible nitric oxide synthase and NO, but also induced HO-1 expression in a concentration-dependent manner; in vivo, it increased survival and decreased the HMGB1 levels of serum and lung in CLP-induced sepsis [80]. In another study, the anti-inflammatory activity, the anti-nociceptive activity, and the wound healing activity of 121 were studied using a carrageenan-induced hind paw edema model in vivo, a p-benzoquinone-induced abdominal constriction test, and incision and excision models in vivo, respectively [81]. It was previously reported that 121 was more active than ibuprofen in the writhing test (67.6% and 50.0% at equimolar doses) and showed similar effects in the tail flick (topic and oral) at equivalent dose to ibuprofen [82].…”

Section: Anti-inflammatory Effectmentioning

confidence: 99%

Phenylethanoid Glycosides: Research Advances in Their Phytochemistry, Pharmacological Activity and Pharmacokinetics

2016

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Phenylethanoid glycosides (PhGs) are widely distributed in traditional Chinese medicines as well as in other medicinal plants, and they were characterized by a phenethyl alcohol (C 6 -C 2 ) moiety attached to a β-glucopyranose/β-allopyranose via a glycosidic bond. The outstanding activity of PhGs in diverse diseases proves their importance in medicinal chemistry research. This review summarizes new findings on PhGs over the past 10 years, concerning the new structures, their bioactivities, including neuroprotective, anti-inflammatory, antioxidant, antibacterial and antivirus, cytotoxic, immunomodulatory, and enzyme inhibitory effects, and pharmacokinetic properties.

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“…Various biological compounds such as phenolic acids, iridoids, phenylpropanoids, flavonoids, saponins, and terpenoids have been isolated from this genus (5-10). These compounds possess anti-inflammatory, antibacterial, immunomodulatory, cardiovascular, diuretic, molluscicidal, cytotoxic, and antitumor properties (11)(12)(13)(14)(15). In traditional Chinese medicine, extract of S. ningpoensis Hemsl has been used in the treatment of fever, constipation, pharyngitis, and neuritis (16).…”

Section: Introductionmentioning

confidence: 99%

The Seasonal Variations of the Chemical Composition of Essential Oil Obtained From Scrophularia frigida

Asgharian

Afshar

Asnaashari

et al. 2016

Jundishapur J Nat Pharm Prod

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Background: In general, seasonal growing of plants influences chemical composition and biological activities of essential oils. Objectives: Therefore, the aerial parts of Scrophularia frigida (S. frigida) were used in the current study to find seasonal variations in the content and composition of essential oil. Materials and Methods:The oil was extracted by hydro-distillation from two samples collected in different seasons, and analyzed by GC-FID and GC-MS. Results: In total, 18 and 20 components were identified and quantified in the oil of summer and autumn samples representing 97.42% and 94.77% of the oil components, respectively. The essential oil of autumn sample was mainly composed of fatty acid derivatives (42.69%). The major components of the autumn oil were palmitic acid (30.49%), phytol (12.99%), L-linalool (11.41%), and hexahydrofarnesyl acetone (6.65%). The essential oil of summer sample was dominated by oxygenated monoterpenes (68.91%), L-linalool (38.69%), geraniol (11.20%), α-terpineol (9.99%), and palmitic acid (7.32%). Conclusions: Based on these observations, wide variation is detected in the chemical composition of the oil obtained from the same plant in different seasons.

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Bioassay-guided isolation of anti-inflammatory, antinociceptive and wound healer glycosides from the flowers of Verbascum mucronatum Lam.

Cited by 70 publications

References 17 publications

Correlation between polyphenol content and anti-inflammatory activity ofVerbascum phlomoides(mullein)

Correlation between polyphenol content and anti-inflammatory activity ofVerbascum phlomoides(mullein)

Phenylethanoid Glycosides: Research Advances in Their Phytochemistry, Pharmacological Activity and Pharmacokinetics

The Seasonal Variations of the Chemical Composition of Essential Oil Obtained From Scrophularia frigida

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