Bioassay-guided isolation, identification of compounds from
            <i>Origanum rotundifolium</i>
            and investigation of their antiproliferative and antioxidant activities

Erenler, Ram­azan; Meral, Bilal; Sen, Özkan; Elmastaş, Mahfuz; Aydın, Ali; Eminağaoğlu, Özgür; Topcu, Gülaçtı

doi:10.1080/13880209.2017.1310906

Cited by 79 publications

(37 citation statements)

References 45 publications

(34 reference statements)

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“…10 µL suspended cell was mixed with 10 µL trypan blue, then 10 µL of this mixture was pipetted to Thoma cell counting chamber to determine the cell concentration. The cell stock suspension was prepared into the 15 mL of falcon tube, then pippeted into the cell plates (5 ×10 3 cells/well) [35,36].…”

Section: Cell Cultures Preparationmentioning

confidence: 99%

Inhibition of Various Cancer Cells Proliferation of Bornyl Acetate and Essential Oil from Inula graveolens (Linnaeus) Desf.

Karan¹,

Yildiz²,

Aydın³

et al. 2018

Rec.Nat.Prod.

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Inula species are medicinal and aromatic plants used for folk medicine extensively. In this work, hydrodistilled essential oil of Inula graveolens (Linnaeus) Desf. analyzed by GC-MS which revealed that bornyl acetate was the major product (69.15%). Camphene was the second major compound (11.11%). Antiproliferative activity of the essential oil and bornyl acetate was investigated on HeLa (human cervix carcinoma), HT29 (human colon carcinoma), A549 (human lung carcinoma), MCF7 (human breast adenocarcinoma) cancer cells and FL (human amnion cells) normal cells. The cytotoxicity was executed by a Lactate Dehydrogenase (LDH) Cytotoxicity Detection Kit. Essential oil and bornyl acetate displayed the outstanding activities on HeLa (IC 50 , 64.1, 72.0 µg/mL), HT29 (IC 50 , 24.6, 60.5 µg/mL), A549 (IC 50 , 28.3,44.1 µg/mL), MCF-7 (IC 50 , 66.5, 85.6 µg/mL), and FL (IC 50 , 42.1, 50.6 µg/mL) cell lines respectively.

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Section: Cell Cultures Preparationmentioning

confidence: 99%

Inhibition of Various Cancer Cells Proliferation of Bornyl Acetate and Essential Oil from Inula graveolens (Linnaeus) Desf.

Karan¹,

Yildiz²,

Aydın³

et al. 2018

Rec.Nat.Prod.

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“…Plants play a significant role in drug discovery process. Since they reveal plenty of pharmaceutical properties, isolation of bioactive compounds from plants gains the great interest to find the most effective compounds with activity mechanism Demirtas et al, 2013;Elmastas et al, 2016;Elmastaş et al, 2015;Erenler, Meral, et al, 2017;Erenler et al, 2018;Guzel et al, 2017;Karan et al, 2017;Karan & Erenler 2017a;Karan & Erenler 2017b;Topçu et al, 1999;Yaglıoglu et al, 2013;Yildiz et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antioxidant capacity with total phenolic content of Galanthus krasnovii (Amaryllidaceae)

Erenler

Genç

Elmastaş

et al. 2019

Turkish Journal of Biodiversity

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Natural products have gained the great interest due to their broad spectrum of biological activities. Galanthus krasnovii was dried at shade then extracted with hexane, dichloromethane, and ethyl acetate successively. After removing of solvent by reduced pressure, crude extracts of each solvent were yielded. Antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation and reducing power assays were carried out for each extract. Moreover, total phenolic content was determined. Total phenolic content of hexane-, dichloromethane-, and ethyl acetate extracts were found as 60.95 mg GAE/g extract, 71.90 GAE/g extract and 58.90 GAE/g extract respectively. Dichloromethane and ethyl acetate extract revealed the moderate antioxidant activity. ÖZDoğal ürünler geniş spektrumlu biyolojik aktivite göstermelerinden dolayı yoğun ilgi görmektedirler. Galanthus krasnovii gölgede kurutulduktan sonra sırasıyla hekzan, diklorometan ve etil asetat ile ekstrakte edildi. Düşük basınçta çözücü uzaklaştırıldıktan sonra her bir çözücünün ekstraktı elde edildi. Her bir ekstraktın, 1,1-difenil-2-pikralhidrazil(DPPH) radikal, 2,2'-azino-bis(3-etilbenzotiazolin-6sülfirik asit) (ABTS) radikal katyon ve indirgeme gücü antioksidan çalışmaları gerçekleştirildi. Ayrıca, ekstraktların toplam fenolik içerikleri belirlendi. Hekzan-, diklorometan-ve etil asetat ekstraktlarının toplam fenolik içerikleri sırasıyla 60.95 mg GAE/g ekstrakt, 71.90 GAE/g ekstrakt ve 58.90 GAE/g ekstrakt olarak belirlendi. Diklorometan ve etil asetat ekstraktları orta derece aktivite gösterdi. Citation:To cite this article: Erenler R, Genç N, Elmastaş M, Eminağaoğlu Ö (2019). Evaluation of antioxidant capacity with total phenolic content of Galanthus krasnovii (Amaryllidaceae). Turk J Biod 2(1): 13-17.Evaluation of antioxidant capacity with total phenolic content of Galanthus krasnovii 14 | Erenler R, Genç N, Elmastaş M, Eminağaoğlu Ö (2019). Turkish Journal of Biodiversity 2(1): 13-17

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“…Due to the including of bioactive compounds, isolation and identification of corresponding compounds have gained the great interest lately. Since the natural compounds have a little or lack of side effect as a drug, they inspired to synthetic chemists to synthesize natural compounds for pharmaceutical industry (Erenler, Meral, et al, 2017;.…”

Section: Introductionmentioning

confidence: 99%

Galanthus woronowii (Amaryllidaceae)'nin antioksidan aktivitesi ve toplam fenolik içeriği.

Genç

Yildiz

Karan

et al. 2019

Turkish Journal of Biodiversity

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Plants have been used for medicinal purpose since ancient times. Due to the including bioactive secondary metabolites, plants have gained the great interest for drug discovery and development process. In this work, Galanthus woronowii was extracted with hexane, dichloromethane and ethyl acetate sequentially. After removing of the solvent by rotary evaporator, crude extracts were yielded. Antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3ethylbenzothiazoline-6-sulphonic acid) (ABTS) and reducing power assays were executed on corresponding extracts. In addition, total phenolic content was presented. Ethyl acetate extract included the most phenolic compounds and also it revealed the most antioxidant activity. Hence, this plant could be considered as a promising antioxidant agent. ÖZ Bitkiler eski çağlardan beri tıbbi amaçlarla kullanılmaktadır. İçerdikleri biyoaktif sekonder metabolitlerden dolayı, bitkiler ilaç keşfi ve gelişimi için oldukça fazla ilgi görmektedir. Bu çalışmada, Galanthus woronowii türüne ait bitki materyalleri sırasıyla hekzan, diklorometan ve etil asetat ile ekstrakte edilmiştir. Çözücü dönerli buharlaştırıcı ile uzaklaştırıldıktan sonra ham ekstraktlar elde edilmiştir. Ekstraktların DPPH serbest radikal giderme, ABTS radikal katyon giderme ve indirgeme gücü aktiviteleri değerlendirilmiştir. Ayrıca toplan fenolik içerik belirlenmiştir. Etil asetat ekstraktının en çok fenolik içerdiği ve en yüksek antioksidan aktivite gösterdiği belirlenmiştir. Bu nedenlerle, bu bitki ümit verici antioksidan ajan olarak kabul edilebilir.

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Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities

Abstract: Globoidnan A (6) has the most antioxidant effects on all assays. This has to do with the chemical structure of the compound bearing the acidic protons. Vitexin (3) could be a promising anticancer agent.

Cited by 79 publications

References 45 publications

Inhibition of Various Cancer Cells Proliferation of Bornyl Acetate and Essential Oil from Inula graveolens (Linnaeus) Desf.

Inhibition of Various Cancer Cells Proliferation of Bornyl Acetate and Essential Oil from Inula graveolens (Linnaeus) Desf.

Evaluation of antioxidant capacity with total phenolic content of Galanthus krasnovii (Amaryllidaceae)

Galanthus woronowii (Amaryllidaceae)'nin antioksidan aktivitesi ve toplam fenolik içeriği.

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