Bioassay-guided fractionation and identification of wound healing active compound from Pistacia vera L. hull extract.

Sarkhail, Parisa; Navidpour, Latifeh; Rahimifard, Mahban; Hosseini, Negar Mohammad; Souri, Effat

doi:10.1016/j.jep.2019.112335

Cited by 28 publications

(20 citation statements)

References 42 publications

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“…Cells were incubated with the tested extracts ( JM1 – JM6 ) and compounds ( 9 , 12 , and 22 ) at concentrations of 10, 25, 50, 100, 200 and 300 μg/mL for 24 h at 37°C. Allantoin, a plant-derived commercialized drug, was used as the positive control at a concentration of 50 μg/mL to judge the rate of cell migration ( Sarkhail et al, 2020 ). After incubation, the migrated cells were washed twice with PBS and observed under a phase contrast microscope.…”

Section: Methodsmentioning

confidence: 99%

Wound Healing Properties of Jasione montana Extracts and Their Main Secondary Metabolites

et al. 2022

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The effects of different extracts obtained from Jasione montana L. (JM1–JM6) and their main metabolites on biological processes during wound healing were evaluated. The effect on wound closure in the scratch test was established, and collagen type I synthesis and anti-inflammatory effects were assessed by flow cytometry in a human dermal fibroblast model (PCS-201-012). Additionally, the antioxidant activity (DPPH and FRAP) and degree of inhibition of elastase participating in the proliferation processes of skin fibroblasts were determined in an in vitro model. The extracts and fractions were analyzed using high-performance liquid chromatography–photodiode array detection (HPLC–PDA) to quantitatively characterize their main polyphenolic compounds. The high antioxidant activity of the JM4–JM5 fractions correlated with the content of luteolin and its derivative 7-O-glucoside. Luteolin also showed the highest anti-elastase activity with an IC50 value of 39.93 ± 1.06 μg/mL, and its substantial content in the JM4 fraction presumably determines its activity (359.03 ± 1.65 μg/mL). At lower concentrations (<50 μg/mL) of all extracts, cell proliferation and migration were significantly stimulated after 24 h of treatment. The stimulation of cell migration was comparable with that of allantoin, which was used as a positive control. However, most of the tested extracts showed limited capacity to affect collagen type I biosynthesis. Moreover, the tested samples exhibited a complex effect on cytokine secretion, and the strongest anti-inflammatory activity through the moderation of IL-1β, IL-6 and IL-8 was observed for JM4 and luteolin. Based on the obtained results of the quantitative analysis, the anti-inflammatory activity of JM4 may be due to the high content of luteolin. In summary, extracts from J. montana, which is flavonoid-rich, promote the viability and accelerate the migration of fibroblasts as well as moderate oxidant and inflammatory processes and elastase activity. Hence, they may be potentially useful for topical therapeutic applications to stimulate the wound healing process.

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Section: Methodsmentioning

confidence: 99%

Wound Healing Properties of Jasione montana Extracts and Their Main Secondary Metabolites

et al. 2022

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“…Other compounds which exhibited antiplasmodial action include 3,4,3 -tri-O-methylellagic acid, triterpenes: arjunolic acid, arjungenin, and arjunic acid, and phenolic glycoside calophymembranside-B [68]. In addition to the above-mentioned reports, a number of investigations were performed by the natural chemists; where a bioactivity-guided fractionation approach was used for successful isolation of new or previously identified phytoconstituents [69][70][71][72][73][74].…”

Section: Some Recent Experiments Using Bio-activity-guided Fractionation Techniquementioning

confidence: 99%

Modern Approaches in the Discovery and Development of Plant-Based Natural Products and Their Analogues as Potential Therapeutic Agents

et al. 2022

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Natural products represents an important source of new lead compounds in drug discovery research. Several drugs currently used as therapeutic agents have been developed from natural sources; plant sources are specifically important. In the past few decades, pharmaceutical companies demonstrated insignificant attention towards natural product drug discovery, mainly due to its intrinsic complexity. Recently, technological advancements greatly helped to address the challenges and resulted in the revived scientific interest in drug discovery from natural sources. This review provides a comprehensive overview of various approaches used in the selection, authentication, extraction/isolation, biological screening, and analogue development through the application of modern drug-development principles of plant-based natural products. Main focus is given to the bioactivity-guided fractionation approach along with associated challenges and major advancements. A brief outline of historical development in natural product drug discovery and a snapshot of the prominent natural drugs developed in the last few decades are also presented. The researcher’s opinions indicated that an integrated interdisciplinary approach utilizing technological advances is necessary for the successful development of natural products. These involve the application of efficient selection method, well-designed extraction/isolation procedure, advanced structure elucidation techniques, and bioassays with a high-throughput capacity to establish druggability and patentability of phyto-compounds. A number of modern approaches including molecular modeling, virtual screening, natural product library, and database mining are being used for improving natural product drug discovery research. Renewed scientific interest and recent research trends in natural product drug discovery clearly indicated that natural products will play important role in the future development of new therapeutic drugs and it is also anticipated that efficient application of new approaches will further improve the drug discovery campaign.

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“…The mastic extract, seed oil and resin oil of P. lenticus have been tested as significant wound healing agent in different experimental models [69][70][71]. Bioassay -guided isolation and identification of various chemical constitutes of P. vera has been reported [72]. The topical wound healing gel has been formulated with significant effect of P. atlantica [73].…”

Section: Wound Healingmentioning

confidence: 99%

Pharmacological Investigation of Genus Pistacia

Rauf¹,

Al-Awthan²,

Muhammad³

et al. 2022

Natural Medicinal Plants

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Several plants in the genus Pistacia are used in the treatment of various pathogenic and non-pathogenic disorders. Especially important are the major species belonging to this genus such as Pistacia lentiscus, Pistacia atlantica, Pistacia vera, Pistacia terebinthus, and Pistacia khinjuk, among others; these have been reported for their potential benefits both in medical and commercial purposes. In addition, members of this genus exhibit numerous ethnomedicinal uses, such as analgesic, anti-inflammatory, anticancer, antimicrobial, antihypertension, antihyperlipidemic, antiviral, and antiasthma. In light of these potential uses, the present chapter aimed to collect and summarize the literature about all of this medicinal information. Accordingly, this chapter focuses on the pharmacological uses and benefits of the genus Pistacia, especially those related to health issues.

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Bioassay-guided fractionation and identification of wound healing active compound from Pistacia vera L. hull extract.

Cited by 28 publications

References 42 publications

Wound Healing Properties of Jasione montana Extracts and Their Main Secondary Metabolites

Wound Healing Properties of Jasione montana Extracts and Their Main Secondary Metabolites

Modern Approaches in the Discovery and Development of Plant-Based Natural Products and Their Analogues as Potential Therapeutic Agents

Pharmacological Investigation of Genus Pistacia

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