Bioassay guided fractionation-an emerging technique influence the isolation, identification and characterization of lead phytomolecules

Malviya, Neelesh; Malviya, Sapna

doi:10.28933/ijhp-2017-07-0901

Cited by 28 publications

(25 citation statements)

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“…Natural products have received great prominence in recent years due to the promising anti‐inflammatory and immunomodulating activity of extracts from plants and marine organisms and their isolated compounds . Some of the leading pharmaceutical and research institutions have demonstrated growing interest in investigating plants to discover medically useful compounds . This study is part of an ongoing investigation on the chemical composition and immunomodulating properties of Brazilian green propolis (BGP) and its main botanical source, Baccharis dracunculifolia D.C. (Asteraceae) …”

Section: Introductionmentioning

confidence: 99%

“…The selection of suitable extracts for the production of commercial products for a given therapeutic application requires not only the knowledge of the extracts’ chemical composition and chemical markers, but also the availability of bioassays to monitor their biological activity . In this sense, to develop BdE‐based anti‐inflammatory medicines, it is important to continue investigating how it modulates the effector functions of immune cells through different experimental approaches.…”

Section: Introductionmentioning

confidence: 99%

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Baccharis dracunculifoliaDC (Asteraceae) selectively modulates the effector functions of human neutrophils

Figueiredo-Rinhel

Melo

Bortot

et al. 2017

Journal of Pharmacy and Pharmacology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Baccharis dracunculifoliaDC (Asteraceae) selectively modulates the effector functions of human neutrophils

Figueiredo-Rinhel

Melo

Bortot

et al. 2017

Journal of Pharmacy and Pharmacology

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“…Importantly, the in vitro antifungal phenotypes observed were consistent; observed in independent isolates, across duplicate assays and under two different culture environment conditions. Thus, while beyond the scope of this study, the bioactive compounds should be discoverable using bioassay guided fractionation and isolation [ 70 , 80 , 81 ].…”

Section: Discussionmentioning

confidence: 99%

Novel Antifungal Activity of Lolium-Associated Epichloë Endophytes

et al. 2020

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Asexual Epichloë spp. fungal endophytes have been extensively studied for their functional secondary metabolite production. Historically, research mostly focused on understanding toxicity of endophyte-derived compounds on grazing livestock. However, endophyte-derived compounds also provide protection against invertebrate pests, disease, and other environmental stresses, which is important for ensuring yield and persistence of pastures. A preliminary screen of 30 strains using an in vitro dual culture bioassay identified 18 endophyte strains with antifungal activity. The novel strains NEA12, NEA21, and NEA23 were selected for further investigation as they are also known to produce alkaloids associated with protection against insect pests. Antifungal activity of selected endophyte strains was confirmed against three grass pathogens, Ceratobasidium sp., Dreschlera sp., and Fusarium sp., using independent isolates in an in vitro bioassay. NEA21 and NEA23 showed potent activity against Ceratobasidium sp. and NEA12 showed moderate inhibition against all three pathogens. Crude extracts from liquid cultures of NEA12 and NEA23 also inhibited growth of the phytopathogens Ceratobasidium sp. and Fusarium sp. and provided evidence that the compounds of interest are stable, constitutively expressed, and secreted. Comparative analysis of the in vitro and in planta metabolome of NEA12 and NEA23 using LCMS profile data revealed individual metabolites unique to each strain that are present in vitro and in planta. These compounds are the best candidates for the differential bioactivity observed for each strain. Novel endophyte strains show promise for endophyte-mediated control of phytopathogens impacting Lolium spp. pasture production and animal welfare.

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“…This is to achieve a specific class of bioactive metabolites or, preferentially, a unique and well-defined bioactive molecule. This process is called bioassay-guided fractionation and is emerging in the field of drug discovery from natural sources [201]. This approach usually starts with different extracts obtained by using solvents with different polarities, in order to have a set of extracts enriched in different types of metabolites.…”

Section: Lipidomics For the Analysis Of Bioactive Lipids In Plants Anmentioning

confidence: 99%

“…The bioassay-fractionation-bioassay sequence can go around until a promising candidate molecule or family of molecules are considered and then it is characterized. The first step of fractionation is typically a liquid–liquid extraction (solvent–solvent partition), and normally uses chromatography techniques, as TLC and column chromatography [ 201 , 202 , 203 ]. Isolated fractions are assayed afterwards.…”

Section: Lipidomics For the Analysis Of Bioactive Lipids In Plantsmentioning

confidence: 99%

Antimicrobial Lipids from Plants and Marine Organisms: An Overview of the Current State-of-the-Art and Future Prospects

et al. 2020

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In the actual post-antibiotic era, novel ways of rethinking antimicrobial research approaches are more urgent than ever. Natural compounds with antimicrobial activity such as fatty acids and monoacylglycerols have been investigated for decades. Additionally, the interest in other lipid classes as antimicrobial agents is rising. This review provides an overview on the research about plant and marine lipids with potential antimicrobial activity, the methods for obtaining and analyzing these compounds, with emphasis on lipidomics, and future perspectives for bioprospection and applications for antimicrobial lipids. Lipid extracts or lipids isolated from higher plants, algae or marine invertebrates are promising molecules to inactivate a wide spectrum of microorganisms. These lipids include a variety of chemical structures. Present and future challenges in the research of antimicrobial lipids from natural origin are related to the investment and optimization of the analytical workflow based on lipidomics tools, complementary to the bioassay-guided fractionation, to identify the active compound(s). Also, further work is needed regarding the study of their mechanism of action, the structure–activity relationship, the synergistic effect with conventional antibiotics, and the eventual development of resistance to lipids, which, as far as is known, is unlikely.

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Bioassay guided fractionation-an emerging technique influence the isolation, identification and characterization of lead phytomolecules

Abstract: N e e l e s h M a l v i y a a n d S a p n a Malviya. Bioassay guided fractionation-an emerging technique influence the isolation, identification and characterization of lead phytomolecules.

Cited by 28 publications

References 4 publications

Baccharis dracunculifoliaDC (Asteraceae) selectively modulates the effector functions of human neutrophils

Baccharis dracunculifoliaDC (Asteraceae) selectively modulates the effector functions of human neutrophils

Novel Antifungal Activity of Lolium-Associated Epichloë Endophytes

Antimicrobial Lipids from Plants and Marine Organisms: An Overview of the Current State-of-the-Art and Future Prospects

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