Bioassay for Diagnosis of Plant Viruses

Browning, Isla A.

doi:10.1007/978-1-59745-062-1_1

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…Thirty‐five of the most widely grown potato cultivars in Scotland by seed acreage were grown from pathogen‐free in vitro plantlets obtained from the SASA potato nuclear stock collection. Plants were inoculated mechanically, as previously described (Browning, ) with sap extract from PYBV isolate QV276 and PVA isolate QV282 propagated in Nicotiana benthamiana (1:5 w/v in water). Three or four potato plants per cultivar (six leaves per plant) were inoculated for each virus, and two plants per cultivar were inoculated with sap from healthy potato (cv.…”

Section: Methodsmentioning

confidence: 99%

Biological and molecular characterization of Potato yellow blotch virus, a new species of the genus Potyvirus

et al. 2018

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A new species of the genus Potyvirus infecting potatoes, with the proposed name Potato yellow blotch virus (PYBV), was discovered in a breeding line 99m‐022‐026 in Scotland. The infected plants show isolated yellow blotches on the leaves. The genome of PYBV contains a large open reading frame encoding a single polyprotein of 3054 amino acids. Sequence analysis shows that PYBV is closely related to Potato virus A (PVA), with an overall 72% identity at the nucleotide level for the whole genome. The least conserved P1 protease gene shares only 50% nucleotide identity with PVA. The host range of PYBV was comparable to PVA on solanaceaous and non‐solanaceous indicator plant species with the exception of Solanum demissum A and Y. Different symptoms were also observed for PYBV and PVA in Nicotiana benthamiana, Nicotiana hesperis and Nicotiana occidentalis P1. The susceptibility of potato (Solanum tuberosum) cultivars to PYBV and PVA was similar. In over 5 years of investigation, PYBV has not been found in commercial seed and ware potato crops in Scotland.

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Section: Methodsmentioning

confidence: 99%

Biological and molecular characterization of Potato yellow blotch virus, a new species of the genus Potyvirus

et al. 2018

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“…Monitoring and surveillance programs must be accompanied by appropriate serological or molecular diagnostic methods [ 75 ], in addition to the characterization of the genetic diversity and population structure since novel variants can emerge. The monitoring based on symptom expression is often technically challenging, whereas different viruses induce similar symptoms, some plant species may be asymptomatic, or symptoms can even be mistaken for physiological disorders or nutrient deficiencies [ 76 ]. Serological methods, such as enzyme-linked immunosorbent assay (ELISA) and its variants, have been used for decades, allowing the identification and detection of viruses in a fast and easy manner [ 77 ].…”

Section: Geographical Distribution and Occurrence Of Aphid-transmitte...mentioning

confidence: 99%

Occurrence, Distribution, and Management of Aphid-Transmitted Viruses in Cucurbits in Spain

2023

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The effectiveness of pest and disease management in crops relies on knowledge about their presence and distribution in crop-producing areas. Aphids and whiteflies are among the main threats to vegetable crops since these hemipterans feed on plants, causing severe damage, and are also able to transmit a large number of devastating plant viral diseases. In particular, the widespread occurrence of aphid-transmitted viruses in cucurbit crops, along with the lack of effective control measures, makes surveillance programs and virus epidemiology necessary for providing sound advice and further integration into the management strategies that can ensure sustainable food production. This review describes the current presence and distribution of aphid-transmitted viruses in cucurbits in Spain, providing valuable epidemiological information, including symptom expressions of virus-infected plants for further surveillance and viral detection. We also provide an overview of the current measures for virus infection prevention and control strategies in cucurbits and indicate the need for further research and innovative strategies against aphid pests and their associated viral diseases.

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“…For infection biotesting we took leaves from symptomatic plants of maize "Mosquito" and sweet maize "Brusnytsia", homogenized them with a mortar in 0.05 M potassium phosphate buffer pH 7.0 (ratio 1:10) and inoculated directly on young plant leaves (5 weeks old) with carborundum as an abrasive [42]. Three weeks after infection, the results were recorded.…”

Section: Maize Leaf Samples and Plant Inoculationmentioning

confidence: 99%

First Report of Sugarcane Mosaic Virus in Zea mays L. in Ukraine

Snihur¹,

Харина²,

Kaliuzhna³

et al. 2021

Mikrobiol. Z.

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Maize viral diseases especially maize dwarf mosaic disease (MDMD), which is caused by potyviruses, lead to significant crop losses worldwide. Aim. The aim of this work was to identify the causal agent of mosaic symptoms, observed on maize plants during 2018—2020 in Kyiv region. Methods. Enzyme-linked immunosorbent assay in the DAS-ELISA modification using commercial Loewe Biochemica test systems for Maize dwarf mosaic virus (MDMV), Sugarcane mosaic virus (SCMV), Wheat streak mosaic virus (WSMV) were applied to identify the causal agent of maize disease in collected samples. Transmission electron microscopy was used in order to direct viral particle visualisation. Aphids, which are natural vectors of plant viruses, were found on diseased plants. Results. Plants with typical mosaic symptoms were observed in corn crops of the Kyiv region in early June 2018. The pathogen was transmitted by mechanical inoculation to maize and sweet maize plants with the manifestation of mosaic symptoms. Electron microscopy of the sap from diseased plants revealed the presence of flexible filamentous virions 750 nm long and 13 nm in diameter, typical for the genus Potyvirus. In August, mosaic symptoms and aphids Rhopalosiphum padi were found on previously healthy plants in the same maize crop. In 2020, in the same sown area, maize plants were free of viral infection during inspection in June, but a re-inspection in September revealed mosaic symptoms on maize crop and the presence of aphids in the leaf axils. The presence of SCMV in maize samples collected in June and August/September 2018 and 2020, as well as in inoculated maize and sweet maize plants, was confirmed by ELISA using a commercial test system. The obtained data allow suggesting that Rhopalosiphum padi is a natural vector of SCMV in agrocenoses of Ukraine. It should be noted that co-infection with MDMV and WSMV in the affected plants was not detected. Conclusions. This study presents the first report of SCMV in maize in Ukraine.

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Bioassay for Diagnosis of Plant Viruses

Abstract: Bioassay provides a useful means of detecting and identifying plant viruses. The choice of procedure and environmental conditions for the test depends on the virus under investigation.

Cited by 6 publications

References 24 publications

Biological and molecular characterization of Potato yellow blotch virus, a new species of the genus Potyvirus

Biological and molecular characterization of Potato yellow blotch virus, a new species of the genus Potyvirus

Occurrence, Distribution, and Management of Aphid-Transmitted Viruses in Cucurbits in Spain

First Report of Sugarcane Mosaic Virus in Zea mays L. in Ukraine

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