“…HEPs were cultured with a Williams'E medium containing dexamethasone (I jiM, Sigma), ascorbic acid (0.28 mM, Sigma), insulin (0.57 mg/L, Sigma), epidermal growth factor (0.02 mg/L, Sigma), gentamicin (48 mg/L, Schellingplau), and aprotinin (5000 KIU/L, Sigma). Cardiac myocytes (CMYs) were isolated by enzyme treatment of minced, heart tissues of 19-day rat embryos (Sprague Dawley rats; Japan SLC, lnc) [8]. CMYs were cultured with a Hepes-buffered Hams FI0 medium containing 0.5 % insulin-transferrin-selenium-X (Gibco) and 3 % fetal calf serum (Gibco).…”