Bioanalytical Studies of Porphyric Disorders Using HPLC with Fluorescence Detection

Cypriani, Michele G. S. C.; Alves, Atecla Nunciata Lopes; Bechara, Etelvino J.H.; Assunção, Nilson Antônio

doi:10.5935/0103-5053.20140230

Cited by 3 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serine biosynthesis disorders are associated with neurological disorders (Koning et al, ) such as schizophrenia (Devor & Waziri, ), seizures and severe psychomotor retardation (Jaeken et al, ). Threonine is an important amino acid for the metabolism of porphyrin (Cypriani, Alves, Bechara, & Assunção, ), synthesized from Asp and catabolized by the following pathway: conversion to alpha‐ketobutyric acid threonine dehydratase, cleavage to Gly and acetaldehyde by threonine aldolase and hydrogenation and decarboxylation to produce aminoacetone (House, Hall, & Brosnan, ). One study showed an increase in Thr in the serum and urine of 5p− patients, suggesting enzymatic failures which consequently cause disturbances in amino acid metabolism (Kühner et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…However, another study showed (Furtado et al, 2017) Serine biosynthesis disorders are associated with neurological disorders (Koning et al, 2003) such as schizophrenia (Devor & Waziri, 1993), seizures and severe psychomotor retardation (Jaeken et al, 1996). Threonine is an important amino acid for the metabolism of porphyrin (Cypriani, Alves, Bechara, & Assunção, 2014), synthesized from Asp and catabolized by the following pathway:…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Targeted analysis reveals alteration in pathway in 5p minus individuals

Furtado

Leite

Jedlicka

et al. 2020

Biomedical Chromatography

View full text Add to dashboard Cite

Cri du Chat or 5p minus (5p−) syndrome is characterized by a deletion located on the chromosome 5 short (−p) arm and has an incidence rate of 1 in 50,000 individuals worldwide. This disease manifests in disturbances across a range of systems biochemicals. Therefore, a targeted metabolomics analysis was evaluated in patients with 5p− syndrome to help unravel the biochemical changes that occur in this disease. Urine samples were collected from people of both sexes aged 1–38 years old and analyzed by ultra‐performance liquid chromatography coupled to mass spectrometry. Student' statistical test, metabolomic pathway analysis and metabolite set enrichment analysis were applied to the data. Alterations of some amino acids and amine biogenics levels were found in Cri du Chat Syndrome individuals. The alteration of most of these metabolites is associated with energy recuperation and glycolysis. In general, we found the catabolism of some metabolic pathways to be affected in 5p− patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Targeted analysis reveals alteration in pathway in 5p minus individuals

Furtado

Leite

Jedlicka

et al. 2020

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…In this study we report the validation of a simple, relatively low‐cost method, using a nonacid liquid–liquid extraction procedure, followed by the quantification of zinc‐protoporphyrin using HPLC with a fluorescence detector. With good recovery and accuracy, the method is sensitive enough to evaluate this biomarker of Pb effect, as well as applicable in the diagnosis of porphyria (Cypriani, Alves, Bechara, & Assunção, 2014). Furthermore, here we report the application of the analytical method in the preliminary assessment of artisanal potters from a village in Brazil famous for its arts and craft.…”

Section: Discussionmentioning

confidence: 99%

Zinc‐protoporphyrin determination by HPLC with fluorescence detection as a biomarker of lead effect in artisanal pottery workers

Santos

Bandeira

Bah

et al. 2020

Biomedical Chromatography

View full text Add to dashboard Cite

Lead (Pb) exposure compromises heme synthesis by inhibiting ferrochelatase, forming zinc‐protoporphyrin (ZnPP). This study aims to validate a method for the determination of ZnPP by HPLC with fluorescence detection and apply this method to evaluate the extent of Pb exposure of artisanal pottery workers. Extraction procedures were tested using both nonacid and acid liquid–liquid extraction. The former presented a better chromatogram and recovery results. The validated method yielded a good resolution of ZnPP and its free form peaks with acceptable precision and accuracy. Total run time was 15 min and ZnPP peak retention time was 5.6 min. We applied this method to evaluate 39 potters (90% male), mean age 40 years (9–80). The medians (ranges) of blood lead, ZnPP and hemoglobin were 16.0 μg/dl (2.2–71.5), 12.6 μg/dl (4.6–279.8) and 15.1 g/dl (11.0–17.8), respectively. Significant differences were observed for blood lead according to gender, age range (>40 years), direct handling of lead oxide and years of occupation. Significantly higher levels of ZnPP were observed in male potters involved with lead glazing activity. The validated method was shown to be simple with one‐step nonacid extraction, good sensitivity, reproducibility and accuracy. Our data shows that these laborers are dangerously exposed to Pb, reflecting the effect on the heme synthesis.

show abstract

“…Specific monitoring of the concentration of porphyrins was intended to be used for characterization of induced changes in porphyrin metabolism upon treatment. Thus, the quantification of porphyrins, including heme, by chromatographic methods was already proposed in the 1970s. − Analysis of porphyrin extracts from biological media by comparison of the elution profiles with an internal porphyrin standard by HPLC allows for the identification. In principle, two different techniques employing varying porphyrin preparation procedures need to be mentioned: normal-phase-HPLC (NP-HPLC) and reversed-phase-HPLC (RP-HPLC).…”

Section: Chromatographic Analytical Techniques and Capillary Electrop...mentioning

confidence: 99%

Heme Determination and Quantification Methods and Their Suitability for Practical Applications and Everyday Use

et al. 2020

View full text Add to dashboard Cite

Many research institutions, clinical diagnostic laboratories, and blood banks are desperately searching for a possibility to identify and quantify heme in different physiological and pathological settings as well as various research applications. The reasons for this are the toxicity of the heme and the fact that it acts as a hemolytic and pro-inflammatory molecule. Heme only exerts these severe and undesired effects when it is not incorporated in hemoproteins. Upon release from the hemoproteins, it enters a biologically available state (labile heme), in which it is loosely associated with proteins, lipids, nucleic acids, or other molecules. While the current methods and procedures for quantitative determination of heme have been used for many years in different settings, their value is limited by the challenging chemical properties of heme. A major cause of inadequate quantification is the separation of labile and permanently bound heme and its high aggregation potential. Thus, none of the current methods are utilized as a generally applicable, standardized approach. The aim of this Feature is to describe and summarize the most common and frequently used chemical, analytical, and biochemical methods for the quantitative determination of heme. Based on this overview, the most promising approaches for future solutions to heme quantification are highlighted.

show abstract

Bioanalytical Studies of Porphyric Disorders Using HPLC with Fluorescence Detection

Cited by 3 publications

References 0 publications

Targeted analysis reveals alteration in pathway in 5p minus individuals

Targeted analysis reveals alteration in pathway in 5p minus individuals

Zinc‐protoporphyrin determination by HPLC with fluorescence detection as a biomarker of lead effect in artisanal pottery workers

Heme Determination and Quantification Methods and Their Suitability for Practical Applications and Everyday Use

Contact Info

Product

Resources

About