2020
DOI: 10.1021/acs.analchem.0c00415
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Heme Determination and Quantification Methods and Their Suitability for Practical Applications and Everyday Use

Abstract: Many research institutions, clinical diagnostic laboratories, and blood banks are desperately searching for a possibility to identify and quantify heme in different physiological and pathological settings as well as various research applications. The reasons for this are the toxicity of the heme and the fact that it acts as a hemolytic and pro-inflammatory molecule. Heme only exerts these severe and undesired effects when it is not incorporated in hemoproteins. Upon release from the hemoproteins, it enters a b… Show more

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Cited by 35 publications
(25 citation statements)
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References 116 publications
(296 reference statements)
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“…In order to selectively measure concentrations of exchangeable heme, it is necessary to use a method that is compatible with in vivo fluorescence imaging or spectroscopy of live cells. 174 Such an approach is possible by the design and application of genetically encoded heme sensors which can be expressed recombinantly in different cell lines.…”
Section: Quantifying Heme Concentrations In Cellsmentioning
confidence: 99%
“…In order to selectively measure concentrations of exchangeable heme, it is necessary to use a method that is compatible with in vivo fluorescence imaging or spectroscopy of live cells. 174 Such an approach is possible by the design and application of genetically encoded heme sensors which can be expressed recombinantly in different cell lines.…”
Section: Quantifying Heme Concentrations In Cellsmentioning
confidence: 99%
“…Heme-STING1 complexes were separated by gel electrophoresis in non-denaturing running buffer. Peroxidase-like activity of heme (31) bound to STING1 was detected on the gel after incubation with TMB Substrate Reagent Set (BD Biosciences). After a blue-colored band developing, the gel were washed with 0.5 M sodium acetate (pH=5) and isopropanol (30%).…”
Section: Methodsmentioning
confidence: 99%
“…Total cellular heme in yeast and various non-erythroid human cell lines is on the order of 1-20 µM (62)(63)(64)(65)(66)(67). All heme in the cell partitions between exchange inert high affinity In the present report, using the model human cell line, HEK293 cells, we sought to determine if endogenous LH is sufficiently accessible and abundant for changes in HO-2 expression to impact heme degradation and establish the oxidation state of LH.…”
Section: Introductionmentioning
confidence: 99%
“…Total cellular heme in yeast and various nonerythroid human cell lines is on the order of 1 to 20 μM ( 62 , 63 , 64 , 65 , 66 , 67 ). All heme in the cell partitions between exchange inert high affinity hemoproteins, such as cytochromes and other heme enzymes, and certain exchange labile heme (LH) complexes that buffer free heme down to nanomolar concentrations ( 8 , 12 , 13 , 14 , 63 , 68 , 69 ).…”
mentioning
confidence: 99%